Recombinant Rat C-C motif chemokine 4 protein (Ccl4) (Active)

CCL4 (MIP-1β) is a key CC chemokine that drives monocyte and lymphocyte recruitment to sites of inflammation, making it an essential tool for dissecting leukocyte trafficking mechanisms. This tag-free recombinant rat CCL4 encompasses the full-length mature sequence (residues 24–92) and demonstrates confirmed biological activity in a monocyte chemotaxis bioassay at concentrations of 10–1000 ng/ml, providing a suitable basis for migration assays, CCR5 signaling pathway studies involving MAPK and NF-κB activation, and in vivo rat models of inflammation or infection. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound immune cell functional readouts, satisfying the stringent criteria typical for primary monocyte and PBMC-based experiments. The protein's >95% purity by SDS-PAGE further supports its use as a reliable reference standard in cytokine detection assays or as a positive control for antibody validation by ELISA and western blot.