Matrix metallopeptidase 13 (MMP13), also known as collagenase 3, is a zinc-dependent endopeptidase that plays an important role in extracellular matrix remodeling and tissue homeostasis. This enzyme shows high specificity for type II collagen and is particularly important in cartilage degradation processes. MMP13 is widely studied in research related to arthritis, osteoarthritis, and other degenerative joint diseases, where its dysregulation contributes to pathological cartilage breakdown. The enzyme is also involved in normal physiological processes including bone development, wound healing, and tissue repair mechanisms.
The Rat matrix metalloproteinase 13,MMP-13 ELISA kit (CSB-E07412r) is designed for quantitative measurement of MMP13 in Rattus norvegicus samples. This sandwich ELISA works with serum, plasma, and tissue homogenates with a detection range of 93.75 pg/mL to 6000 pg/mL and sensitivity of 23.44 pg/mL. The assay requires 50-100ul sample volume and can be completed within 1-5 hours. Detection occurs at 450 nm wavelength, providing researchers with a reliable tool for MMP13 quantification in rat samples.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research examining inflammatory processes and cartilage degradation, particularly in the context of osteoarthritis studies. The kit allows quantification of matrix metalloproteinase 13 in plasma samples, serving as a specific indicator of cartilage damage in inflammatory conditions.
• Osteoarthritis research: Measurement of cartilage damage biomarkers in plasma samples from animal models of joint inflammation and degeneration
• Inflammatory biomarker profiling: Quantification of matrix metalloproteinase levels alongside other inflammatory markers in studies examining disease-associated inflammation
• Cartilage metabolism studies: Analysis of collagenase activity and collagen degradation processes in experimental models of joint disease
