Name: Recombinant Rat Collagenase 3 (Mmp13)
Brand: CUSABIO
SKU: 014660RA

Product Details

Purity

>85% (SDS-PAGE)

Target Names

Mmp13

Uniprot No.

P23097

Alternative Names

Mmp13; Collagenase 3; EC 3.4.24.-; Matrix metalloproteinase-13; MMP-13; UMRCASE; Fragment

Species

Rattus norvegicus (Rat)

Expression Region

99-466

Target Protein Sequence

YN VFPRTLKWSQ TNLTYRIVNY TPDISHSEVE KAFRKAFKVW SDVTPLNFTR IHDGTADIMI SFGTKEHGDF YPFDGPSGLL AHAFPPGPNL GGDAHFDDDE TWTSSSKGYN LFIVAAHELG HSLGLDHSKD PGALMFPIYT YTGKSHFMLP DDDVQGIQSL YGPGDEDPNP KHPKTPEKCD PALSLDAITS LRGETMIFKD RFFWRLHPQQ VEPELFLTKS FWPELPNHVD AAYEHPSRDL MFIFRGRKFW ALNGYDIMEG YPRKISDLGF PKEVKRLSAA VHFEDTGKTL FFSGNHVWSY DDANQTMDKD YPRLIEEEFP GIGDKVDAVY EKNGYIYFFN GPIQFEYSIW SNRIVRVMPT NSLLWC

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

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Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

Bacterial collagenase might be causing inflammation in rat. we are interested to inject collagease of rat origin in rat to avoid any inflammation.
Any suggestions?

Very nice to receive your inquiry. CSB-MP014666RA/CSB-MP014660RA On how to avoid to be stimulated heterologous protein expression of animal produce inflammation, we haven't done related research generally exogenous substances stimulation animal, because of the mammalian immune system more perfect, generally stimulate mammals to produce the corresponding reaction to eliminate the effect of foreign matter but the protein itself is the source of rats, the expression systems is the mammalian cells, to the most close to maintain its natural protein structure, and rats of protein expression differences will not very big, may cause the rejection of rats is much smaller.

Target Background

Function

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.

Subcellular Location

Secreted, extracellular space, extracellular matrix. Secreted.

Protein Families

Peptidase M10A family

Database Links

STRING: 10116.ENSRNOP00000011507

UniGene: Rn.10997

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Recombinant Rat Collagenase 3 (Mmp13)

Product Details

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Customer Reviews and Q&A

Target Background

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