Code | CSB-E12118r |
Size | 96T,5×96T,10×96T |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
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Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<15% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 92 | ||||
Range % | 88-96 | |||||
1:2 | Average % | 88 | ||||
Range % | 85-90 | |||||
1:4 | Average % | 100 | ||||
Range % | 97-103 | |||||
1:8 | Average % | 83 | ||||
Range % | 80-86 |
The recovery of rat SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 87 | 82-91 | ||||
EDTA plasma (n=4) | 95 | 89-100 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||
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The Rat sex hormone-binding globulin (SHBG) ELISA kit is a high-quality research tool for measuring SHBG levels in Rattus norvegicus (rat) samples. This ELISA kit is designed to accurately quantify SHBG levels in a range of sample types including serum, plasma, cell culture supernates, tissue homogenates, and cell lysates.
The Rat SHBG ELISA kit has a detection range of 375 ng/mL-6000 ng/mL and a sensitivity of 375 ng/mL, making it a highly precise tool for researchers studying sex function,thyroid/liver function and signal transduction pathways. With an assay time of 1-5 hours and a sample volume requirement of 50-100 ul, this kit is both efficient and cost-effective.
The assay principle of the Rat SHBG ELISA kit is quantitative and based on a competitive measurement. The kit utilizes a high-quality antibody specific to SHBG, ensuring accurate and reliable results. The detection wavelength is 450 nm, providing a clear and precise readout of results.
This ELISA kit has been widely cited in research publications, with over 5 citations to date. Researchers across the globe trust the accuracy and precision of the Rat SHBG ELISA kit, making it a must-have tool for any lab studying sex function,thyroid/liver function and signal transduction pathways.
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