Rat sex hormone-binding globulin (SHBG) ELISA kit

Instructions
Code CSB-E12118r
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name sex hormone-binding globulin
Alternative Names Shbg ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit
Abbreviation SHBG
Uniprot No. P08689
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 375 ng/mL-6000 ng/mL
Sensitivity 375 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 88-96  
1:2 Average % 88  
Range % 85-90  
1:4 Average % 100  
Range % 97-103  
1:8 Average % 83  
Range % 80-86  
Recovery
The recovery of rat SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 87 82-91  
EDTA plasma (n=4) 95 89-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
6000 0.204 0.201 0.203    
3000 0.336 0.341 0.339    
1500 0.789 0.772 0.781    
750 1.800 1.806 1.803    
375 2.312 2.353 2.333    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration (By similarity).
Gene References into Functions
  1. SHBG may be of functional importance for rapid effects of olfactory steroids on limbic functions including the control of reproductive behaviours through pheromones PMID: 24681170
  2. Fluorine had no effect on the expression of androgen binding protein and inhibin B mRNA in testis sertoli cells of Sprague Dawley rats. PMID: 21033444
  3. Upstream stimulatory factors (USF1 and USF2) induce Nr5a1 and Shbg gene expression during the onset of rat Sertoli cell differentiation. PMID: 21734262
  4. Data show that the use of alternative SHBG first exons 1A and 1B differentially inhibits translation from the ATG situated in exon 2, which codes for methionine 30 of transcripts that begin with the exon 1 sequence. PMID: 21079794
  5. Results suggest that Sertoli cell-derived androgen-binding protein acts on spermatids by modifying the transition protein-1 mRNA level. PMID: 12573823
  6. A wave of apoptosis during the pre-pubertal period is required for normal spermatogenesis to develop, and our data indicate that this apoptotic wave may be regulated by ABP and/or androgens. PMID: 12831397
  7. ABP was found in neurons of the magnocellular hypothalamic nuclei, the preoptic region and the lateral hypothalamus. ABP varicosities occurred throughout the hypothalamus, the median eminence and the posterior pituitary. PMID: 14625054
  8. ABP expression is regulated in differentiated Sertoli cells by the bHLH and CREB family of transcription factors. PMID: 15112319
  9. Estrogen receptor beta was strongly immunolabeled in pachytene spermatocytes of rat androgen-binding protein transgenic mice and localized in tubular stages in which TUNEL labeling was maximal. PMID: 15215204
  10. There is a significantly higher level of the Shbg transcript in hypodactylous rats compared to SHR controls. PMID: 15222128
  11. Intrahypothalamic expression of ABP may be among the mechanisms necessary for rapid actions of steroids on hypothalamic neuroendocrine systems. PMID: 15927785

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Subcellular Location Secreted
Tissue Specificity Isoform 2 is only expressed in the liver.
Database Links

KEGG: rno:24775

STRING: 10116.ENSRNOP00000015248

UniGene: Rn.37473

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