Recombinant Rat Sex hormone-binding globulin (Shbg)

Code CSB-YP021252RA
MSDS
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Source Yeast
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Code CSB-EP021252RA
MSDS
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Source E.coli
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Code CSB-EP021252RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021252RA
MSDS
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Source Baculovirus
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Code CSB-MP021252RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Shbg
Uniprot No.
Alternative Names
Shbg; Sex hormone-binding globulin; SHBG; Sex steroid-binding protein; SBP; Testis-specific androgen-binding protein; ABP
Species
Rattus norvegicus (Rat)
Expression Region
31-403
Target Protein Sequence
LRHIDPIQSA QDSPAKYLSN GPGQEPVTVL TIDLTKISKP SSSFEFRTWD PEGVIFYGDT NTEDDWFMLG LRDGQLEIQL HNLWARLTVG FGPRLNDGRW HPVELKMNGD SLLLWVDGKE MLCLRQVSAS LADHPQLSMR IALGGLLLPT SKLRFPLVPA LDGCIRRDIW LGHQAQLSTS ARTSLGNCDV DLQPGLFFPP GTHAEFSLQD IPQPHTDPWT FSLELGFKLV DGAGRLLTLG TGTNSSWLTL HLQDQTVVLS SEAEPKLALP LAVGLPLQLK LDVFKVALSQ GPKMEVLSTS LLRLASLWRL WSHPQGHLSL GALPGEDSSA SFCLSDLWVQ GQRLDIDKAL SRSQDIWTHS CPQSPSNDTH TSH
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
Gene References into Functions
  1. SHBG may be of functional importance for rapid effects of olfactory steroids on limbic functions including the control of reproductive behaviours through pheromones PMID: 24681170
  2. Fluorine had no effect on the expression of androgen binding protein and inhibin B mRNA in testis sertoli cells of Sprague Dawley rats. PMID: 21033444
  3. Upstream stimulatory factors (USF1 and USF2) induce Nr5a1 and Shbg gene expression during the onset of rat Sertoli cell differentiation. PMID: 21734262
  4. Data show that the use of alternative SHBG first exons 1A and 1B differentially inhibits translation from the ATG situated in exon 2, which codes for methionine 30 of transcripts that begin with the exon 1 sequence. PMID: 21079794
  5. Results suggest that Sertoli cell-derived androgen-binding protein acts on spermatids by modifying the transition protein-1 mRNA level. PMID: 12573823
  6. A wave of apoptosis during the pre-pubertal period is required for normal spermatogenesis to develop, and our data indicate that this apoptotic wave may be regulated by ABP and/or androgens. PMID: 12831397
  7. ABP was found in neurons of the magnocellular hypothalamic nuclei, the preoptic region and the lateral hypothalamus. ABP varicosities occurred throughout the hypothalamus, the median eminence and the posterior pituitary. PMID: 14625054
  8. ABP expression is regulated in differentiated Sertoli cells by the bHLH and CREB family of transcription factors. PMID: 15112319
  9. Estrogen receptor beta was strongly immunolabeled in pachytene spermatocytes of rat androgen-binding protein transgenic mice and localized in tubular stages in which TUNEL labeling was maximal. PMID: 15215204
  10. There is a significantly higher level of the Shbg transcript in hypodactylous rats compared to SHR controls. PMID: 15222128
  11. Intrahypothalamic expression of ABP may be among the mechanisms necessary for rapid actions of steroids on hypothalamic neuroendocrine systems. PMID: 15927785

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Subcellular Location
Secreted. Note=In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis.
Tissue Specificity
Isoform 2 is only expressed in the liver.
Database Links
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