Name: Rat vimentin (VIM)ELISA Kit
Brand: CUSABIO
SKU: CSB-E14029r

Product Details

Alternative Names

Vim ELISA Kit; Vimentin ELISA Kit

Abbreviation

VIM

Uniprot No.

P31000

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

23.5 ng/mL-1500 ng/mL

Sensitivity

5.8 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat VIM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-95
1:2	Average %	93
	Range %	88-98
1:4	Average %	97
	Range %	93-102
1:8	Average %	100
	Range %	95-104

Recovery

The recovery of rat VIM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	95-107
EDTA plasma (n=4)	90	85-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	3.066	2.888	2.977	2.880
5	2.598	2.621	2.610	2.513
2.5	1.958	1.845	1.902	1.805
1.25	0.943	0.978	0.961	0.864
0.625	0.623	0.612	0.618	0.521
0.312	0.366	0.356	0.361	0.264
0.156	0.228	0.229	0.229	0.132
0	0.093	0.101	0.097

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Rat VIM ELISA Kit was designed for the quantitative measurement of Rat VIM protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 ng/mL-1500 ng/mL and the sensitivity is 5.8 ng/mL.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

■ Q&A

Please provide the following information regarding Rat vimentin, VIM ELISA Kit (CSB-E14029r):
a. What is the Uniprot/Accession number that this kit is designed for? Is the kit designed for XYZ species or is it designed for a different species such as Human and cross reacts with XYZ species?
b. Is the capture antibody a monoclonal or polyclonal?
c. What is the host of the capture antibody?
d. Is the detection antibody a monoclonal or polyclonal?
e. What is the host of the detection antibody?
f. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
g. If recombinant protein, is it full length or partial?
h. What is the species of the standard– human or something else?
i. What sample type was tested with this kit (serum? plasma? Something else?) and what were the reference values.

Thanks for your inquiry.
CSB-E14029r
This kit is designed against full length. Pls refer to: https://www.uniprot.org/uniprot/P31000.
Capture antibody is mouse monoclonal antibody.
Detection antibody is sheep polyclonal antibody.
Standard is NS0-derived recombinant protein.
This kit is designed for rat. Although it has homology with the protein from other species, we don't suggest you use this kit to test other species because there is matrix interference.
We tested serum and plasma samples before and the test value is 20--100ng/ml.
Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.; Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Gene References into Functions

We labeled brain with ZO1, vimentin, pan-cadherin and angiotensin II type 1 receptors markers which showed a morphologically distinct population of cells at the region of the sulcus medianus organum similar to tanycytes present in the median eminence, a known circumventricular organ. PMID: 29803674
In transected spinal cord rats, vimentin knockdown in the scar tissues showed a significant improvement on locomotor function in hindlimbs. PMID: 24874717
The increased expression of LP-vimentin-IR cells suggests that changes in cell-cell interactions could play a role in ischemia-induced changes in bladder activity. PMID: 24728265
Double-immunostaining experiments with antibodies against Stk33 and vimentin showed a striking colocalization of Stk33 and vimentin in the hypothalamus. PMID: 24057876
We have identified three protein biomarkers (GSN, MYL2, and VIM) that have potential utility in diagnosing the severity of Chagas disease PMID: 22199233
vimentin is a regulator of NRG1 type III function and peripheral nerve myelination PMID: 22357929
Estradiol treatment leads to an effect on vimentin phosphorylation. PMID: 21915674
These data suggest that the inability of YY1 to access the hypermethylated promoter may be one of the mechanisms that mediate vimentin downregulation. PMID: 22005459
Data show that vimentin intermediate filaments play a crucial role in mechanical behavior not only at large deformations but also in the nanomechanical response of individual cells. PMID: 21426942
Data show that astrocyte structural proteins GFAP and vimentin are induced and by chronic leptin administration, suggesting astrocytes participating in leptin modulated synaptic inputs. PMID: 21343257
GR-mediated reduction in vimentin may be involved in the formation of steroid-induced cataract PMID: 21245955
Increases in post-traumatic vimentin mRNA in the cortex and in the hippocampus appear together with vimentin immunoreactivity in astrocytes starting at one day after severe trauma. PMID: 20479526
Endothelial cell hypoxia causes redistribution of vimentin to a more insoluble and extensive filamentous network that could play a role in endothelial barrier stabilization. PMID: 20427712
Vimentin mRNA and protein expression levels increased significantly throughout hepatotumorigenesis. Vimentin is an important node in underlying gene regulatory networks. PMID: 20015863
Fetal hepatocytes at the epithelial mesenchymal transition have a higher level of this protein than in fetal hepatocytes. PMID: 12496370
induction in glial cells of hippocampus following hyperthermia PMID: 12834255
vimentin may play a key role in the formation of glial scars in the central nervous system PMID: 15684657
demonstrate the alterations of alpha-smooth muscle actin and vimentin localization patterns in the bovine cryptorchid testis as compared to the contralateral scrotal testis during postnatal development PMID: 16493181
Taken together, these results suggest that vimentin is a novel member of binding partner of PP1c in astrocytes, and vimentin-Ser82 may act as a memory phosphorylation site. PMID: 16629905
Vimentin phosphorylation was increased, indicating an accumulation of phosphorylated vimentin associated to the cytoskeleton in hyperthyroidism induced rats. PMID: 17306450
Rats fed the homocysteine diet had an increase in Vim. PMID: 17485853
vimentin-immunopositive cells localize (in both intact and lesioned brain) in the zone of barrier structures' formation. PMID: 18198667
the phosphorylation and the immunoreactivity of cytoskeletal-associated vimentin were increased without altering vimentin expression, suggesting an accumulation of insoluble and phosphorylated vimentin. PMID: 18316471
Single impact load also causes changes in the pattern of vimentin immunoreactivity, indicating vimentin dissassembley in cartilage injury model PMID: 18577232
Vimentin was expressed in cells of the outer and inner capsules of all muscle spindles and in newly formed myoblasts and myotubes of regenerating spindles 7 days after grafting PMID: 18941770
Vimentin was observed within cells located in the primitive ducts of rat pancreas newborn animals. It was also spotted in alpha cells starting from E12.5 but disappeared soon after birth, likely identifying immature or recently differentiated alpha cells. PMID: 19223297

Hide All

Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Nucleus matrix. Cell membrane.

Protein Families

Intermediate filament family

Database Links

KEGG: rno:81818

STRING: 10116.ENSRNOP00000024430

UniGene: Rn.2710

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-E14029r
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Rat vimentin (VIM)ELISA Kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background