VIM

Vimentin (VIM), a type III intermediate filament protein, is a key structural component maintaining cellular integrity and regulating cytoskeletal dynamics. Also known as fibroblastic filament protein, VIM facilitates cell migration, adhesion, and organelle positioning through interactions with actin, microtubules, and integrins. It modulates signaling pathways, including TGF-β/Smad, Wnt/β-catenin, and epithelial-mesenchymal transition (EMT), driving processes like wound healing and cancer metastasis. Dysregulation of VIM is implicated in fibrosis, autoimmune disorders (e.g., rheumatoid arthritis), and tumor progression, particularly in aggressive carcinomas with mesenchymal features.
Targeting VIM has emerged as a therapeutic strategy. Small-molecule inhibitors (e.g., withaferin A) disrupt VIM polymerization, while monoclonal antibodies block extracellular VIM involved in tumor microenvironment crosstalk. Recent trials explore VIM-centric therapies in metastatic cancers and fibrotic diseases, often combined with immune checkpoint inhibitors. Challenges remain in specificity, but CRISPR screening and proteolysis-targeting chimeras (PROTACs) offer precision tools to mitigate off-target effects.

Validated Data

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

VIM Recombinant Monoclonal Antibody (CSB-RA025857MA1HU)

Validated Data

IHC image of CSB-RA025857MA1HU diluted at 1:40 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Rabbit IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA025857MA1HU diluted at 1:40 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Rabbit IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of NIH/3T3 cell with CSB-RA025857MA1HU at 1:40, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Human vimentin,VIM ELISA Kit (CSB-E08982h)

Validated Data

Code: CSB-E08982h

Size: 96T,5×96T,10×96T

Sensitivity: 7.8 pg/mL

Detection Range: 31.25 pg/mL-2000 pg/mL

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following VIM reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

VIM Antibodies

VIM Proteins

VIM ELISA Kits

VIM Antibodies

VIM Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA025857GA01HU	VIM Antibody	Human,Mouse,Rat	ELISA,WB,IHC,IF
CSB-PA573495	VIM Antibody	Human,Mouse,Rat,Monkey	ELISA,WB,IF
CSB-PA084867	Phospho-VIM (Ser56) Antibody	Human	ELISA,WB
CSB-PA233606	VIM Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA000431	VIM Antibody	Human,Mouse,Rat	WB
CSB-PA004448	VIM Antibody	Human,Mouse,Rat	WB, IHC, IF, ELISA
CSB-PA004449	VIM Antibody	Human	WB, IHC, IF, ELISA
CSB-PA004450	VIM Antibody	Human,Mouse,Rat	WB, IHC, IF, ELISA
CSB-PA004451	VIM Antibody	Human	WB, ELISA
CSB-PA060073	Phospho-VIM (Y61) Antibody	Human	WB, ELISA
CSB-PA060074	Phospho-VIM (S83) Antibody	Human,Mouse,Rat	WB, IHC, ELISA
CSB-PA060075	Phospho-VIM (S56) Antibody	Human	WB, IHC, ELISA
CSB-PA224594	VIM Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA200577	VIM Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-MA000319	VIM Monoclonal Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA025857LA01HU	VIM Antibody	Human	ELISA, WB, IHC, IF
CSB-PA025857LB01HU	VIM Antibody, HRP conjugated	Human	ELISA
CSB-PA025857LC01HU	VIM Antibody, FITC conjugated	Human
CSB-PA025857LD01HU	VIM Antibody, Biotin conjugated	Human	ELISA
CSB-RA025857MA1HU	VIM Recombinant Monoclonal Antibody	Human	ELISA, IHC, IF

VIM Antibodies for Mus musculus (Mouse)

Code	Product Name	Species Reactivity	Application
CSB-PA025857ZA01MO	Vim Antibody	Mus musculus	ELISA, WB (ensure identification of antigen)

VIM Proteins

VIM Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP025857MO CSB-BP025857MO CSB-EP025857MO-B	Recombinant Mouse Vimentin (Vim)	Yeast Baculovirus In Vivo Biotinylation in E.coli
CSB-EP025857MO	Recombinant Mouse Vimentin (Vim)	E.coli

VIM Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP025857RA CSB-EP025857RA CSB-BP025857RA CSB-MP025857RA CSB-EP025857RA-B	Recombinant Rat Vimentin (Vim)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

VIM Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP025857BO CSB-EP025857BO CSB-BP025857BO CSB-MP025857BO CSB-EP025857BO-B	Recombinant Bovine Vimentin (VIM)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

VIM Proteins for Gallus gallus (Chicken)

Code	Product Name	Source
CSB-YP025857CH CSB-EP025857CH CSB-BP025857CH CSB-MP025857CH CSB-EP025857CH-B	Recombinant Chicken Vimentin (VIM)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

VIM Proteins for Sus scrofa (Pig)

Code	Product Name	Source
CSB-YP025857PI CSB-EP025857PI CSB-BP025857PI CSB-MP025857PI CSB-EP025857PI-B	Recombinant Pig Vimentin (VIM)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

VIM Proteins for Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops)

Code	Product Name	Source
CSB-YP025857DSU CSB-EP025857DSU CSB-BP025857DSU CSB-MP025857DSU CSB-EP025857DSU-B	Recombinant Chlorocebus aethiops Vimentin (VIM)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

VIM Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

Code	Product Name	Source
CSB-YP025857DXU CSB-EP025857DXU CSB-BP025857DXU CSB-MP025857DXU CSB-EP025857DXU-B	Recombinant Cricetulus griseus Vimentin (VIM)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

VIM Proteins for Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)

Code	Product Name	Source
CSB-YP025857OEI CSB-EP025857OEI CSB-BP025857OEI CSB-MP025857OEI CSB-EP025857OEI-B	Recombinant Oncorhynchus mykiss Vimentin (vim)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

VIM Proteins for Mesocricetus auratus (Golden hamster)

Code	Product Name	Source
CSB-YP025857MRG CSB-EP025857MRG CSB-BP025857MRG CSB-MP025857MRG CSB-EP025857MRG-B	Recombinant Mesocricetus auratus Vimentin (VIM)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

VIM Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Code	Product Name	Source
CSB-YP025857MOV CSB-EP025857MOV CSB-BP025857MOV CSB-MP025857MOV CSB-EP025857MOV-B	Recombinant Macaca fascicularis Vimentin (VIM)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

VIM Proteins for Pan troglodytes (Chimpanzee)

Code	Product Name	Source
CSB-YP025857EQV CSB-EP025857EQV CSB-BP025857EQV CSB-MP025857EQV CSB-EP025857EQV-B	Recombinant Pan troglodytes Vimentin (VIM)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

VIM Proteins for Cyprinus carpio (Common carp)

Code	Product Name	Source
CSB-YP025857EQE CSB-EP025857EQE CSB-BP025857EQE CSB-MP025857EQE CSB-EP025857EQE-B	Recombinant Cyprinus carpio Vimentin (vim)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

VIM Proteins for Ovis aries (Sheep)

Code	Product Name	Source
CSB-YP025857SH CSB-EP025857SH CSB-BP025857SH CSB-MP025857SH CSB-EP025857SH-B	Recombinant Sheep Vimentin (VIM)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

VIM Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-BP025857HU CSB-MP025857HU CSB-EP025857HU-B	Recombinant Human Vimentin (VIM)	Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP025857HU	Recombinant Human Vimentin (VIM)	E.coli
CSB-YP025857HU	Recombinant Human Vimentin (VIM)	Yeast
CSB-EP025857HUc7	Recombinant Human Vimentin (VIM)	E.coli