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| Code | CSB-E08941r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of rat visfatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 82-97 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 96-108 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| The recovery of rat visfatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 98 | 94-108 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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This rat visfatin ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat visfatin in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of visfatin bound in the initial step.
Visfatin (NAMPT) is an adipocytokine that exerts many functions including enhancement of cell proliferation, biosynthesis of nicotinamide mono- and dinucleotide, and hypoglycaemic effect through an endocrine, autocrine, and paracrine manner. Visfatin binds to the insulin receptor at a site different from that of insulin, leading to hypoglycemia by reducing glucose release from liver cells and stimulating glucose utilization in adipocytes and myocytes. Visfatin is upregulated by hypoxia, inflammation, and hyperglycemia but downregulated by insulin, somatostatin, and statins. It is involved in many pathological processes, including obesity, diabetes, and cardiovascular diseases.
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