Rat visfatin ELISA Kit

Instructions
Code CSB-E08941r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name nicotinamide phosphoribosyltransferase
Alternative Names Nampt ELISA Kit; Pbef1 ELISA Kit; Nicotinamide phosphoribosyltransferase ELISA Kit; NAmPRTase ELISA Kit; Nampt ELISA Kit; EC 2.4.2.12 ELISA Kit; Pre-B-cell colony-enhancing factor 1 homolog ELISA Kit; PBEF ELISA Kit; Visfatin ELISA Kit
Abbreviation NAMPT
Uniprot No. Q80Z29
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat visfatin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %87
Range %82-97
1:2Average %96
Range %92-103
1:4Average %102
Range %96-108
1:8Average %94
Range %89-98
Recovery
The recovery of rat visfatin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9590-100
EDTA plasma (n=4)9894-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2001.743 1.867 1.805 1.707
1001.304 1.199 1.252 1.154
500.835 0.838 0.837 0.739
250.534 0.547 0.541 0.443
12.50.348 0.336 0.342 0.244
6.250.277 0.268 0.273 0.175
3.120.171 0.177 0.174 0.076
00.098 0.097 0.098  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component
Gene References into Functions
  1. A Strong association was observed among fasting blood glucose, serum visfatin and trace elements levels of high fat diet and type 2 diabetes mellitus PMID: 29772226
  2. Our findings suggest that NAMPT inhibition may be a novel therapeutic approach for I/R-induced lung injury. The protective effects involve the suppression of multiple signal pathways. PMID: 28438162
  3. Thus, it can be suggested that visfatin may play an important role in testicular aging by regulating testicular steroidogenesis and spermatogenesis. PMID: 28163205
  4. visfatin plays a dual role in the apoptosis in rat Mesenteric lymph nodes, which is mediated by both the mitochondrial apoptotic pathway and the death-receptor apoptotic pathway. PMID: 26857566
  5. this study shows that serum visfatin levels, and white adipose tissue and hepatic visfatin mRNA levels of both male and female rats were increased by LPS injection PMID: 27083000
  6. Visfatin was expressed in the rats' retinas, mainly in the retinal nerve fibre layer, inner plexiform layer and outer plexiform layer. As the development of diabetes mellitus course, its expression was gradually increased. PMID: 26694625
  7. NAMPT protects against 6-hydroxydopamine-induced neurotoxicity in PC12 cells through modulating SIRT1 activity PMID: 27035562
  8. Report retinal toxicity of NAMPT inhibitors. PMID: 25505128
  9. visfatin could participate in the inflammatory process in rat spleen by modulating the macrophages and inflammatory cytokines. PMID: 25358398
  10. Visfatin induced the expression of alphaSMA in cultureactivated rat HSC, which was accompanied by a significant increase in collagen types I and III. PMID: 25351242
  11. These data are the first to show that Nampt inhibits myometrial contractility and does so more potently than leptin. PMID: 25645057
  12. Central visfatin improved glucose homeostasis by increasing insulin secretion and insulin sensitivity at euglycemia through the hypothalamus in diabetic rats. PMID: 24332931
  13. role in myocardial inflammation during endotoxemia PMID: 23831038
  14. Data indicate that nicotinamide phosphoribosyltransferase (NAMPT) mRNA and protein were increased in the kidneys with streptozotocin-induced diabetes. PMID: 24287278
  15. CR-induced beneficial effects in oxidative stress, mitochondrial biogenesis, and metabolic adaptation require NAMPT. PMID: 23946338
  16. visfatin plays a critical role in cardioprotection by suppressing myocardial apoptosis via AMPK activation PMID: 23065734
  17. Nampt promotes neuronal survival through inducing autophagy. PMID: 22113203
  18. Visfatin expression was increased mainly through the AT1-R-JAK/STAT pathway in the process of Ang II-induced cardiomyocyte hypertrophy. PMID: 21841463
  19. The expression of visfatin is closely associated with the expression of proinflammatory cytokines in FFA-induced inflammation and is significantly decreased by NF-kappaB inhibition in HepG2 cells. PMID: 21664630
  20. Our findings reveal that Nampt protects against ischemic stroke through rescuing neurons from death via the SIRT1-dependent AMPK pathway and indicate that Nampt is a new therapeutic target for stroke. PMID: 21246601
  21. results of the present study demonstrate that serum visfatin levels in Lyon hypertensive rats are elevated and are associated with lipid metabolic abnormalities PMID: 20456420
  22. No differences of serum visfatin levels were observed between spontaneously hypertensive rats and Wistar-Kyoto rats. Serum visfatin levels were significantly correlated to weights of skeletal muscle. PMID: 20658215
  23. Visfatin exhibits important autocrine effects on sensitivity of liver cells to insulin action possibly through its effects on NAD biosynthesis. PMID: 19929131
  24. Exercise training induced SIRT1 activity due to the positive effect of exercise on the activity of nicotinamide phosphoribosyltransferase (NAMPT) and thereby the production of sirtuin-fueling NAD(+). PMID: 19913571
  25. PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle PMID: 12782293
  26. relative visfatin gene expression is not associated with the metabolic syndrome in WOKW rats [Pbef, also called visfatin] PMID: 15922301
  27. Results describe the crystal structure of visfatin in three different forms: apo and in complex with either nicotinamide mononucleotide (NMN) or the nicotinamide phosphoribosyltransferase inhibitor FK-866. PMID: 16901503
  28. During fasting, increased Nampt provides protection against cell death and requires an intact mitochondrial NAD(+) salvage pathway as well as the mitochondrial NAD(+)-dependent deacetylases SIRT3 and SIRT4. PMID: 17889652
  29. Visfatin is produced in mesangial cells, which are a novel target for visfatin, and play an important role in the pathogenesis of diabetic nephropathy. PMID: 18768589
  30. improves insulin sensitivity and exerts its hypocholesterolemic effects at least partially through upregulation of the tyrosine phosphorylation of IRS-1 protein and the mRNA levels of PPARgamma and SREBP-2 PMID: 19263259
  31. The crystal structure of rat visfatin in complex with IS001, is desribed. PMID: 19533035
  32. Nicotinamide phosphoribosyltransferase regulates cell survival through NAD+ synthesis in cardiac myocytes. PMID: 19661458

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Subcellular Location Nucleus, Cytoplasm, Secreted
Protein Families NAPRTase family
Tissue Specificity Expressed in various tissues. At the highest level in liver and at the second highest in heart. The amount is higher in heart than in lung.
Database Links

KEGG: rno:297508

STRING: 10116.ENSRNOP00000013043

UniGene: Rn.203508

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