Sheep 5-hydroxytryptamine/serotonin(5HT/ST) ELISA kit

Code CSB-E14929Sh
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
5-hydroxytryptamine/serotonin(5HT/ST)
Abbreviation
5HT/ST
Species
Ovis aries (Sheep)
Sample Types
serum, plasma
Detection Range
0.625 ng/mL-10 ng/mL
Sensitivity
0.312 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Biochemicals
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep 5HT/ST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 84  
Range % 80-92  
1:40 Average % 97  
Range % 91-105  
1:80 Average % 96  
Range % 90-101  
1:160 Average % 93  
Range % 86-98  
Recovery
The recovery of sheep 5HT/ST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 97 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
10 0.122 0.115 0.119    
5 0.234 0.216 0.225    
2.5 0.404 0.407 0.406    
1.25 0.743 0.739 0.741    
0.625 1.221 1.194 1.208    
0 2.051 1.959 2.005    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Sheep 5HT/ST ELISA Kit was designed for the quantitative measurement of Sheep 5HT/ST protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.625 ng/mL-10 ng/mL and the sensitivity is 0.312 ng/mL .

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