Sheep Albumin (Alb)ELISA Kit

Code CSB-E15839Sh
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P14639
Lead Time 3-5 working days
Abbreviation ALB
Protein Biological Process 1 Metabolism
Target Name albumin
Alias DKFZp779N1935, PRO0883, PRO0903, PRO1341, cell growth inhibiting protein 42|growth-inhibiting protein 20|serum albumin
Species Ovis aries (Sheep)
Sample Types serum, plasma, cell culture supernates,Urine, Tissue Homogenates
Detection Range 350 μg/mL-21.88 μg/mL
Sensitivity 5.5 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
HGNC 316
RGD 628614
MGI 2429409
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep albumin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 92
Range % 97-101
1:200 Average % 95
Range % 88-105
1:400 Average % 95
Range % 90-100
1:800 Average % 95
Range % 92-97
Recovery
The recovery of sheep albumin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 87-102
EDTA plasma (n=4) 97 93-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average
350 0.162 0.166 0.164
175 0.281 0.274 0.278
87.5 0.436 0.445 0.441
43.75 0.655 0.667 0.661
21.88 0.923 0.907 0.915
0 2.052 1.996 2.024
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Function Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Subcellular Location Secreted
Protein Families ALB/AFP/VDB family
Tissue Specificity Plasma.
Database Links

KEGG: oas:443393

UniGene: Oar.449

Pathway Th17 cell differentiation
Thyroid hormone synthesis

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