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Sheep Albumin (Alb)ELISA Kit

Instructions
Code CSB-E15839Sh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name albumin
Alternative Names ALBSerum albumin ELISA Kit
Abbreviation ALB
Uniprot No. P14639
Species Ovis aries (Sheep)
Sample Types serum, plasma, cell culture supernates,Urine, Tissue Homogenates
Detection Range 350 μg/mL-21.88 μg/mL
Sensitivity 5.5 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep albumin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)
1:100 Average % 92
Range % 97-101
1:200 Average % 95
Range % 88-105
1:400 Average % 95
Range % 90-100
1:800 Average % 95
Range % 92-97
Recovery
The recovery of sheep albumin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 87-102
EDTA plasma (n=4) 97 93-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average
350 0.162 0.166 0.164
175 0.281 0.274 0.278
87.5 0.436 0.445 0.441
43.75 0.655 0.667 0.661
21.88 0.923 0.907 0.915
0 2.052 1.996 2.024
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Gene References into Functions
  1. We have purified SSA from sheep kidneys by a simple and efficient two-step purification procedure. Further, we have studied urea-induced denaturation of SSA by monitoring changes in the difference absorption coefficient at 287nm intrinsic fluorescence emission intensity and mean residue ellipticity at 222nm at pH 7.4 and 25 degrees C PMID: 27180298
Subcellular Location Secreted
Protein Families ALB/AFP/VDB family
Tissue Specificity Plasma.
Database Links

KEGG: oas:443393

UniGene: Oar.449
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