Sheep Albumin (Alb)ELISA Kit

Code CSB-E15839Sh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Sheep ALB ELISA Kit was designed for the quantitative measurement of Sheep ALB protein in serum, plasma, cell culture supernates,Urine, Tissue Homogenates. It is a Competitive ELISA kit, its detection range is 350 μg/mL-21.88 μg/mL and the sensitivity is 5.5 μg/mL.

Target Name albumin
Alternative Names ALBSerum albumin ELISA Kit
Abbreviation ALB
Uniprot No. P14639
Species Ovis aries (Sheep)
Sample Types serum, plasma, cell culture supernates,Urine, Tissue Homogenates
Detection Range 350 μg/mL-21.88 μg/mL
Sensitivity 5.5 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep albumin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 92
Range % 97-101
1:200 Average % 95
Range % 88-105
1:400 Average % 95
Range % 90-100
1:800 Average % 95
Range % 92-97
Recovery
The recovery of sheep albumin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 87-102
EDTA plasma (n=4) 97 93-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average
350 0.162 0.166 0.164
175 0.281 0.274 0.278
87.5 0.436 0.445 0.441
43.75 0.655 0.667 0.661
21.88 0.923 0.907 0.915
0 2.052 1.996 2.024
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood. The rank order of affinity is zinc > calcium > magnesium. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.
Gene References into Functions
  1. We have purified SSA from sheep kidneys by a simple and efficient two-step purification procedure. Further, we have studied urea-induced denaturation of SSA by monitoring changes in the difference absorption coefficient at 287nm intrinsic fluorescence emission intensity and mean residue ellipticity at 222nm at pH 7.4 and 25 degrees C PMID: 27180298
Subcellular Location Secreted.
Protein Families ALB/AFP/VDB family
Tissue Specificity Plasma.
Database Links

KEGG: oas:443393

UniGene: Oar.449

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