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Sheep Albumin (Alb)ELISA Kit

Sheep Albumin (Alb)ELISA Kit

Specification
Target

Code

CSB-E15839Sh

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Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P14639

Lead Time

3-5 working days

Abbreviation

ALB

Protein Biological Process 1

Metabolism

Target Name

albumin

Alias

DKFZp779N1935, PRO0883, PRO0903, PRO1341, cell growth inhibiting protein 42|growth-inhibiting protein 20|serum albumin

Species

Ovis aries (Sheep)

Sample Types

serum, plasma, cell culture supernates,Urine, Tissue Homogenates

Detection Range

350 μg/mL-21.88 μg/mL

Sensitivity

5.5 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Target Details

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

HGNC

316

RGD

628614

MGI

2429409

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep albumin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	97-101
1:200	Average %	95
	Range %	88-105
1:400	Average %	95
	Range %	90-100
1:800	Average %	95
	Range %	92-97

Recovery

The recovery of sheep albumin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-102
EDTA plasma (n=4)	97	93-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
350	0.162	0.166	0.164
175	0.281	0.274	0.278
87.5	0.436	0.445	0.441
43.75	0.655	0.667	0.661
21.88	0.923	0.907	0.915
0	2.052	1.996	2.024

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Function	Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Subcellular Location	Secreted
Protein Families	ALB/AFP/VDB family
Tissue Specificity	Plasma.
Database Links	KEGG: oas:443393 UniGene: Oar.449
Pathway	Th17 cell differentiation Thyroid hormone synthesis

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ALB Antibody

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ALB Antibodies for Ovis aries (Sheep)

Code	Product Name	Species Reactivity	Application
CSB-PA00050E1Rb	ALB Antibody	Sheep	ELISA
CSB-PA00050F1Rb	ALB Antibody, HRP conjugated	Sheep	ELISA
CSB-PA00050G1Rb	ALB Antibody, FITC conjugated	Sheep	ELISA
CSB-PA00050H1Rb	ALB Antibody, Biotin conjugated	Sheep	ELISA

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ALB Proteins for Ovis aries (Sheep)

Code	Product Name	Source
CSB-YP001561SH CSB-EP001561SH CSB-BP001561SH CSB-MP001561SH	Recombinant Sheep Serum albumin(ALB) ,partial	Yeast E.coli Baculovirus Mammalian cell

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Sheep Albumin (Alb)ELISA Kit

Specification

Target

Related Products

ALB Antibodies

ALB Proteins

Most popular with customers

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