Product Details

Target Name

albumin

Alternative Names

ALBSerum albumin ELISA Kit

Abbreviation

ALB

Uniprot No.

P14639

Species

Ovis aries (Sheep)

Sample Types

serum, plasma, cell culture supernates,Urine, Tissue Homogenates

Detection Range

350 μg/mL-21.88 μg/mL

Sensitivity

5.5 μg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep albumin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	97-101
1:200	Average %	95
	Range %	88-105
1:400	Average %	95
	Range %	90-100
1:800	Average %	95
	Range %	92-97

Recovery

The recovery of sheep albumin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-102
EDTA plasma (n=4)	97	93-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average
350	0.162	0.166	0.164
175	0.281	0.274	0.278
87.5	0.436	0.445	0.441
43.75	0.655	0.667	0.661
21.88	0.923	0.907	0.915
0	2.052	1.996	2.024

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Data

Function	Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Gene References into Functions	We have purified SSA from sheep kidneys by a simple and efficient two-step purification procedure. Further, we have studied urea-induced denaturation of SSA by monitoring changes in the difference absorption coefficient at 287nm intrinsic fluorescence emission intensity and mean residue ellipticity at 222nm at pH 7.4 and 25 degrees C PMID: 27180298
Subcellular Location	Secreted
Protein Families	ALB/AFP/VDB family
Tissue Specificity	Plasma.
Database Links	KEGG: oas:443393 UniGene: Oar.449

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Check Code：

Code	CSB-E15839Sh
Size	96T,5×96T,10×96T
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Sheep Albumin (Alb)ELISA Kit

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