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Sheep Interleukin 10,IL-10 ELISA Kit

Citations (10) Protocol
Code CSB-E12817Sh
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 10
Alternative Names
IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
Abbreviation
IL10
Uniprot No.
Q29408
Species
Ovis aries (Sheep)
Sample Types
serum, plasma, tissue homogenates
Detection Range
5 pg/mL-80 pg/mL
Sensitivity
5 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 92  
Range % 87-98  
1:2 Average % 98  
Range % 93-104  
1:4 Average % 84  
Range % 82-88  
1:8 Average % 90  
Range % 85-97  
Recovery
The recovery of sheep IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 91-99  
EDTA plasma (n=4) 92 87-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
80 0.202 0.196 0.199    
40 0.365 0.345 0.355    
20 0.623 0.641 0.632    
10 1.147 1.168 1.158    
5 2.344 2.189 2.267    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

Interleukin 10 (IL-10) is a potent anti-inflammatory cytokine that plays an important role in immune regulation and homeostasis. Regulatory T cells, macrophages, and B cells produce IL-10 primarily. IL-10 works to suppress inflammatory responses by blocking pro-inflammatory cytokine production and reducing antigen presentation. This immunosuppressive activity makes IL-10 necessary for preventing excessive immune activation. It also helps maintain tissue integrity during inflammatory processes. Research into IL-10 levels offers valuable insights into immune system function, inflammatory disease progression, and therapeutic monitoring in various pathological conditions.

The Sheep Interleukin 10 ELISA Kit (CSB-E12817Sh) measures IL-10 quantitatively in sheep samples using a competitive assay principle. This kit works with serum, plasma, and tissue homogenates. The detection range spans 5 pg/mL to 80 pg/mL with a sensitivity of 5 pg/mL. The assay needs 50-100 μL sample volume and takes 1-5 hours to complete. Detection occurs at 450 nm wavelength, giving researchers a reliable tool for IL-10 quantification in Ovis aries specimens.

Application Examples

Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.

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Citations

Related Products

IL10 Proteins

Customer Reviews and Q&A

■ Customer Reviews
Average Rating:
5.0 - 1 reviews

Submit a Review here

Sample type: Biological fluids(synovial fluid)

Sample species: sheep

Sample dilution: No dilution

Review: I used the CSB ELISA IL10 kit to detect IL-10 in synovial fluid of sheep. I tried it on normal samples, and osteoarthritis samples. I used a multiskan, with automatic conversion of absorbance, after automatic analysis (four parameter logistic curve-fit). I did not have a multichannel pipet so my values are not perfectly repeatable. The kit is easy to use, and protocol is clear. Just keep in mind that you have to read the plates at 450nm and correct with a 620-nm read. It is actually mentioned in the “other supplies required” section, but it is not remind in the “assay procedure” section, “assay procedure summary” section or in the “calculation of result” section (so during the assay there is a risk to forget the 2nd read).

By Anonymous

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Target Background

Function
(From Uniprot)
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
Gene References into Functions
  1. The aim of the study was to assess the prognostic significance of Tumor Necrosis Factor-alpha, Interleukin-12(p40), Interleukin-6 and Interleukin 10 levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. PMID: 25817420
  2. Significant differences in mitogen-specific lymphocyte proliferation in vitro and in expression of TGF-beta1 and IL-10 genes in hepatic lymph nodes were observed at acute and chronic phases of infection, respectively. PMID: 19751473
Subcellular Location
Secreted.
Protein Families
IL-10 family
Database Links

KEGG: oas:443342

UniGene: Oar.528

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Related pathways
Jak-STAT signaling pathway T cell receptor signaling pathway FoxO signaling pathway
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