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| Code | CSB-E12817Sh |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:4 | Average % | 84 | ||||
| Range % | 82-88 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-97 | |||||
| The recovery of sheep IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 92 | 87-97 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||
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This Sheep IL10 ELISA Kit was designed for the quantitative measurement of Sheep IL10 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 5 pg/mL-80 pg/mL and the sensitivity is 5 pg/mL.
Sample type: Biological fluids(synovial fluid)
Sample species: sheep
Sample dilution: No dilution
Review: I used the CSB ELISA IL10 kit to detect IL-10 in synovial fluid of sheep. I tried it on normal samples, and osteoarthritis samples. I used a multiskan, with automatic conversion of absorbance, after automatic analysis (four parameter logistic curve-fit). I did not have a multichannel pipet so my values are not perfectly repeatable. The kit is easy to use, and protocol is clear. Just keep in mind that you have to read the plates at 450nm and correct with a 620-nm read. It is actually mentioned in the “other supplies required” section, but it is not remind in the “assay procedure” section, “assay procedure summary” section or in the “calculation of result” section (so during the assay there is a risk to forget the 2nd read).
By Anonymous
KEGG: oas:443342
UniGene: Oar.528
