Sheep Interleukin 10,IL-10 ELISA Kit

Instructions
Code CSB-E12817Sh
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name interleukin 10
Alternative Names IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
Abbreviation IL10
Uniprot No. Q29408
Species Ovis aries (Sheep)
Sample Types serum, plasma, tissue homogenates
Detection Range 5 pg/mL-80 pg/mL
Sensitivity 5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 87-98  
1:2 Average % 98  
Range % 93-104  
1:4 Average % 84  
Range % 82-88  
1:8 Average % 90  
Range % 85-97  
Recovery
The recovery of sheep IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 91-99  
EDTA plasma (n=4) 92 87-97  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
80 0.202 0.196 0.199    
40 0.365 0.345 0.355    
20 0.623 0.641 0.632    
10 1.147 1.168 1.158    
5 2.344 2.189 2.267    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

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Sample type: Biological fluids(synovial fluid)

Sample species: sheep

Sample dilution: No dilution

Review: I used the CSB ELISA IL10 kit to detect IL-10 in synovial fluid of sheep. I tried it on normal samples, and osteoarthritis samples. I used a multiskan, with automatic conversion of absorbance, after automatic analysis (four parameter logistic curve-fit). I did not have a multichannel pipet so my values are not perfectly repeatable. The kit is easy to use, and protocol is clear. Just keep in mind that you have to read the plates at 450nm and correct with a 620-nm read. It is actually mentioned in the “other supplies required” section, but it is not remind in the “assay procedure” section, “assay procedure summary” section or in the “calculation of result” section (so during the assay there is a risk to forget the 2nd read).

By Anonymous

Target Data

Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
Gene References into Functions
  1. The aim of the study was to assess the prognostic significance of Tumor Necrosis Factor-alpha, Interleukin-12(p40), Interleukin-6 and Interleukin 10 levels in cerebrospinal fluid (CSF) in sheep with encephalitic listeriosis. PMID: 25817420
  2. Significant differences in mitogen-specific lymphocyte proliferation in vitro and in expression of TGF-beta1 and IL-10 genes in hepatic lymph nodes were observed at acute and chronic phases of infection, respectively. PMID: 19751473
Subcellular Location Secreted
Protein Families IL-10 family
Database Links

KEGG: oas:443342

UniGene: Oar.528

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