Sheep Luteinizing Hormone(LH)ELISA Kit

Instructions
Code CSB-E14019Sh
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Luteinizing Hormone(LH)
Alternative Names LHBLutropin subunit beta ELISA Kit; Lutropin beta chain ELISA Kit; Interstitial cell-stimulating hormone ELISA Kit; Luteinizing hormone subunit beta ELISA Kit; LH-B ELISA Kit; LSH-B ELISA Kit; LSH-beta) [Cleaved into: LH beta-1; LH beta-2; LH beta-3] ELISA Kit
Abbreviation LH
Uniprot No. P01231
Species Ovis aries (Sheep)
Sample Types serum, plasma, tissue homogenates
Detection Range 2 mIU/mL-75 mIU/mL
Sensitivity 0.5 mIU/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision

Intra-assay Precision (Precision within an assay): CV%<15%

 

Three samples of known concentration were tested twenty times on one plate to assess.

 

Inter-assay Precision (Precision between assays): CV%<15%

 

Three samples of known concentration were tested in twenty assays to assess.

 

 

 

Linearity

 To assess the linearity of the assay, samples were spiked with high concentrations of Sheep Luteinizing Hormone(LH) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Typical Data

 These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The LH alpha 3/LH beta 3 heterodimer was shown to have potent renotropic and weak gonadotropic activity.
Gene References into Functions
  1. Data indicate that the neuropeptide RFamide-related peptide 3 (RFRP-3) has no effect onluteinizing hormone secretion. PMID: 26862995
  2. This study indicates that endogenous opiates play a crucial role in the mechanism inhibiting GnRH/LH axis activity in lactating sheep and that the ligands for mu opioid receptor may have the highest inhibitory effect. PMID: 23953813
  3. Data suggest dietary components (phytoestrogens here) down-regulate basal secretion/total production of follicle stimulating hormone (CGA+FSHB) yet up-regulate gonadotropin-releasing hormone agonist-induced LH (CGA+LHB) secretion in gonadotrophs. PMID: 24050980
  4. These results indicate that the reproductive system can respond acutely to infusion of metabolic fuels such as propionate, although a sustained recovery of pulsatile LH secretion requires more than an increase in this single metabolic substrate. PMID: 21668532
  5. In ewes, infusion with genistein decreased the level of LHbeta in the adenohypophysis. PMID: 15531133

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Subcellular Location Secreted
Protein Families Glycoprotein hormones subunit beta family
Database Links

KEGG: oas:443395

UniGene: Oar.442

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