Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of Sheep Luteinizing Hormone(LH) in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The LH alpha 3/LH beta 3 heterodimer was shown to have potent renotropic and weak gonadotropic activity.
Gene References into Functions
Data indicate that the neuropeptide RFamide-related peptide 3 (RFRP-3) has no effect onluteinizing hormone secretion. PMID: 26862995
This study indicates that endogenous opiates play a crucial role in the mechanism inhibiting GnRH/LH axis activity in lactating sheep and that the ligands for mu opioid receptor may have the highest inhibitory effect. PMID: 23953813
Data suggest dietary components (phytoestrogens here) down-regulate basal secretion/total production of follicle stimulating hormone (CGA+FSHB) yet up-regulate gonadotropin-releasing hormone agonist-induced LH (CGA+LHB) secretion in gonadotrophs. PMID: 24050980
These results indicate that the reproductive system can respond acutely to infusion of metabolic fuels such as propionate, although a sustained recovery of pulsatile LH secretion requires more than an increase in this single metabolic substrate. PMID: 21668532
In ewes, infusion with genistein decreased the level of LHbeta in the adenohypophysis. PMID: 15531133