Human Beta-Endorphin,β-EP ELISA Kit

Code CSB-E06821h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Abbreviation β-EP
Species Human
Sample Types serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Availability 3-5 working days
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 80-92  
1:2 Average % 99  
Range % 91-110  
1:4 Average % 97  
Range % 92-105  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of human β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.537 2.568 2.553 2.443  
2000 2.010 1.999 2.005 1.895  
1000 1.600 1.532 1.566 1.456  
500 1.032 1.122 1.077 0.967  
250 0.567 0.600 0.584 0.474  
125 0.391 0.410 0.401 0.291  
62.5 0.242 0.254 0.248 0.138  
0 0.109 0.111 0.110    
References
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