Human Beta-Endorphin,β-EP ELISA Kit

Human Beta-Endorphin,β-EP ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Human Beta-Endorphin,β-EP ELISA Kit

Name: Human Beta-Endorphin,β-EP ELISA Kit
Brand: CUSABIO
SKU: CSB-E06821h

Citations (8) Protocol MSDS

Code	CSB-E06821h
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details
Citations (8)
Related Products
Customer Reviews and Q&A

Target Name

Beta-Endorphin,β-EP

Abbreviation

β-EP

Species

Homo sapiens (Human)

Sample Types

serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates

Detection Range

62.5 pg/mL-4000 pg/mL

Sensitivity

15.6 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	99
	Range %	91-110
1:4	Average %	97
	Range %	92-105
1:8	Average %	93
	Range %	86-98

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.537	2.568	2.553	2.443
2000	2.010	1.999	2.005	1.895
1000	1.600	1.532	1.566	1.456
500	1.032	1.122	1.077	0.967
250	0.567	0.600	0.584	0.474
125	0.391	0.410	0.401	0.291
62.5	0.242	0.254	0.248	0.138
0	0.109	0.111	0.110

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Human β-EP ELISA Kit was designed for the quantitative measurement of Human β-EP protein in serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

Measuring of serum taurine and beta endorphin in women as a pre-early marker for diagnosis of cancer breast SA Dardier,/,2023
Effects of circuit-type resistance training on industrial opiate induced neurotransmitter disruption and performance changes of previously addicted men H Arazi,Biomedical Human Kinetics,2020
In major depression, increased kappa and mu opioid receptor levels are associated with immune activation Al-Hakeim H K, et al,Acta Neuropsychiatrica,2019
In Major Depression, Increased Serum Dynorphin and Kappa Opioid Receptor Levels are Positively Associated with Mu Opioid Receptor Levels and Immune Activation and Are Attenuated by Nicotine Dependence Hussein Al-Hakeim, et al,Preprints,2019
Development of a Novel Neuro-Immune and Opioid-Associated Fingerprint with a Cross-Validated Ability to Identify and Authenticate Unknown Patients with Major Depression: Far Beyond Differentiation, Discrimination and Classification Hussein Al-Hakeim, et al,Preprints,2019
IL-10 is associated with increased mu-opioid receptor levels in major depressive disorder Al-Fadhel SZ, et al,European Psychiatry,2019
Sweating away depression? The impact of intensive exercise on depression. Balchin R.et al,J Affect Disord.,2016
Hypomagnetic field alters circadian rhythm and increases algesia in adult male mice MO Wei-Chuan. et al,Progress in Biochemistry and Biophysics,2015

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■ Q&A

I would like to ask about the Human Beta-Endorphin ELISA Kit (CSB-E06821h). In the specification and the manual, I can't understand the difference between the “sensitivity 15.6 pg/mL” and the “detection range of the assay is from 62.5 pg/mL to 4,000 pg/mL”. Is 15.6 pg/mL the minimum detectable level in this ELISA kit? If so, why was the minimum detectable level (15.6 pg/mL) lower than the lowest level of the range (62.5 pg/mL)?

The sensitivity of 15.6pg/ml is the minimum detectable level of this kit, which is obtained by the O.D value of 20 replicates of the zero standard plus their three standard deviations. It is undetectable below this parameter (15.6pg/ml). The measured values of samples in the range of 15.6-62.5pg/ml have large variability. 62.5pg/ml represents the concentration point of the standard S1, and 62.5-4000pg/mL is the detection range of the kit.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.