Product Name:

human Beta-Endorphin,β-EP ELISA Kit

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Product Type : ELISA Kit
Code : CSB-E06821h
Size : 96T,5×96T,10×96T
Abbreviation : β-EP
Species : Human
Sample Types : serum, urine, cerebrospinal fluid (CSF), saliva, tissue homogenates
Detect Range : 62.5 pg/ml-4000 pg/ml
Sensitivity : 15.6 pg/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Research Area : Cancer
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of human β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 86  
Range % 80-92  
1:2 Average % 99  
Range % 91-110  
1:4 Average % 97  
Range % 92-105  
1:8 Average % 93  
Range % 86-98  
Recovery :
The recovery of human β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-99  
             
             
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.537 2.568 2.553 2.443  
2000 2.010 1.999 2.005 1.895  
1000 1.600 1.532 1.566 1.456  
500 1.032 1.122 1.077 0.967  
250 0.567 0.600 0.584 0.474  
125 0.391 0.410 0.401 0.291  
62.5 0.242 0.254 0.248 0.138  
0 0.109 0.111 0.110    
Reference :

1. Hypomagnetic field alters circadian rhythm and increases algesia in adult male mice,MO Wei-Chuan. et al,Progress in Biochemistry and Biophysics,2015,42(7):639-646

2. Sweating away depression? The impact of intensive exercise on depression.,Balchin R.et al,J Affect Disord.,200: 218-221.

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