Rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit

Code CSB-E06858Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Rabbit Col Ⅰ ELISA Kit was designed for the quantitative measurement of Rabbit Col Ⅰ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Name Collagen Type Ⅰ,Col Ⅰ
Alternative Names Collagen Type 1,Col 1,Collagen Type I,Col I
Abbreviation Col Ⅰ
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-89  
1:2 Average % 90  
Range % 85-96  
1:4 Average % 88  
Range % 84-92  
1:8 Average % 104  
Range % 100-109  
The recovery of rabbit Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 90-96  
EDTA plasma (n=4) 99 94-103  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.013 2.136 2.075 1.935  
5 1.749 1.705 1.727 1.587  
2.5 1.388 1.498 1.443 1.303  
1.25 1.065 1.154 1.110 0.970  
0.625 0.754 0.788 0.771 0.631  
0.312 0.513 0.532 0.523 0.383  
0.156 0.312 0.331 0.322 0.182  
0 0.139 0.141 0.140    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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