Rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit

Rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit

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Rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit

Name: Rabbit Collagen Type Ⅰ,Col Ⅰ ELISA Kit
Brand: CUSABIO
SKU: CSB-E06858Rb

^Instructions

Code	CSB-E06858Rb
Size	96T,5×96T,10×96T
Trial Size
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Target Name

Collagen Type Ⅰ,Col Ⅰ

Alternative Names

Collagen Type 1,Col 1,Collagen Type I,Col I

Abbreviation

Col Ⅰ

Species

Oryctolagus cuniculus (Rabbit)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	90
	Range %	85-96
1:4	Average %	88
	Range %	84-92
1:8	Average %	104
	Range %	100-109

Recovery

The recovery of rabbit Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	99	94-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.013	2.136	2.075	1.935
5	1.749	1.705	1.727	1.587
2.5	1.388	1.498	1.443	1.303
1.25	1.065	1.154	1.110	0.970
0.625	0.754	0.788	0.771	0.631
0.312	0.513	0.532	0.523	0.383
0.156	0.312	0.331	0.322	0.182
0	0.139	0.141	0.140

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.