| Description |
This Rabbit Col Ⅰ ELISA Kit was designed for the quantitative measurement of Rabbit Col Ⅰ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL. |
| Target Name |
Collagen Type Ⅰ,Col Ⅰ |
| Alternative Names |
Collagen Type 1,Col 1,Collagen Type I,Col I |
| Abbreviation |
Col Ⅰ |
| Species |
Oryctolagus cuniculus (Rabbit) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.156 ng/mL-10 ng/mL |
| Sensitivity |
0.039 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction
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| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
81-89 |
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| 1:2 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:4 |
Average % |
88 |
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| Range % |
84-92 |
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| 1:8 |
Average % |
104 |
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| Range % |
100-109 |
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| Recovery |
| The recovery of rabbit Col Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
90-96 |
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| EDTA plasma (n=4) |
99 |
94-103 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.013 |
2.136 |
2.075 |
1.935 |
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| 5 |
1.749 |
1.705 |
1.727 |
1.587 |
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| 2.5 |
1.388 |
1.498 |
1.443 |
1.303 |
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| 1.25 |
1.065 |
1.154 |
1.110 |
0.970 |
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| 0.625 |
0.754 |
0.788 |
0.771 |
0.631 |
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| 0.312 |
0.513 |
0.532 |
0.523 |
0.383 |
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| 0.156 |
0.312 |
0.331 |
0.322 |
0.182 |
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| 0 |
0.139 |
0.141 |
0.140 |
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| ELISA Data Analysis |
Watch ELISA data processing video & download Curve Expert if needed |
Troubleshooting
and FAQs |
ELISA kit FAQs |
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
