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Rabbit Soluble protein-100B,S-100B ELISA Kit

Protocol
Code CSB-E06895Rb
Size 96T,5×96T,10×96T
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Product Details

Alternative Names
S100B ELISA Kit; Protein S100-B ELISA Kit; S-100 protein beta chain ELISA Kit; S-100 protein subunit beta ELISA Kit; S100 calcium-binding protein B ELISA Kit
Abbreviation
S100B
Uniprot No.
Q6YNR6
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
　 Sample Serum(n=4)    
1:1 Average % 88    
Range % 84-92    
1:2 Average % 94    
Range % 90-99    
1:4 Average % 103    
Range % 99-110    
1:8 Average % 93    
Range % 89-98    
Recovery
The recovery of rabbit S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 95 90-100    
EDTA plasma (n=4) 97 92-104    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
2000 2.505 2.652 2.579 2.480    
1000 1.968 1.976 1.972 1.873    
500 1.274 1.204 1.239 1.140    
250 0.713 0.739 0.726 0.627    
125 0.436 0.424 0.430 0.331    
62.5 0.251 0.259 0.255 0.156    
31.25 0.182 0.185 0.184 0.085    
0 0.097 0.101 0.099 　    
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Related Products

S100B Proteins

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Target Background

Function
(From Uniprot)
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
Gene References into Functions
  1. The time course of S100B serum values following spinal cord decompression correlates with outcome; the initial degree of paresis is not a prognostic factor to predict outcome. PMID: 21535942
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
S-100 family
Database Links
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