Rabbit basic fibroblast growth factor,bFGF ELISA Kit

Code CSB-E06938Rb
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
fibroblast growth factor 2 (basic)
Alternative Names
FGF2 ELISA Kit; Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit; Fragment ELISA Kit
Abbreviation
FGF2
Uniprot No.
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %87
Range %84-90
1:2Average %104
Range %100-108
1:4Average %85
Range %81-89
1:8Average %99
Range %95-102
Recovery
The recovery of rabbit bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8380-86
EDTA plasma (n=4)9794-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5001.863 1.812 1.838 1.679
2501.699 1.598 1.649 1.490
1251.428 1.436 1.432 1.273
62.51.115 1.168 1.142 0.983
31.20.804 0.831 0.818 0.659
15.60.563 0.574 0.569 0.410
7.80.362 0.372 0.367 0.208
00.159 0.158 0.159
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rabbit FGF2 ELISA Kit was designed for the quantitative measurement of Rabbit FGF2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4. Also acts as an integrin ligand which is required for FGF2 signaling. Binds to integrin ITGAV:ITGB3. Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration. Functions as a potent mitogen in vitro. Can induce angiogenesis. Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation.
Subcellular Location
Secreted. Nucleus.
Protein Families
Heparin-binding growth factors family
Database Links
CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1