Home
Active Protein
Enzyme
Recombinant Human Persephin protein (PSPN), partial (Active)

Recombinant Human Persephin protein (PSPN), partial (Active)

^{In Stock}

Code	CSB-AP002951HU
Abbreviation	Recombinant Human PSPN protein, partial (Active)
MSDS	MSDS Datasheet
Size	$142
	Order now
Image
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Purity

>97% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TT medullary thyroid cancer cells is less than 10 ng/ml, corresponding to a specific activity of >1.0x10⁵ IU/mg.

Target Names

PSPN

Uniprot No.

O60542

Research Area

Neuroscience?

Alternative Names

PSPN; Persephin; PSP

Species

Homo sapiens (Human)

Source

E.Coli

Expression Region

61-156aa

Complete Sequence

ALSGPCQLWS LTLSVAELGL GYASEEKVIF RYCAGSCPRG ARTQHGLALA RLQGQGRAHG GPCCRPTRYT DVAFLDDRHR WQRLPQLSAA ACGCGG

Mol. Weight

10.3 kDa

Protein Length

Partial

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered PBS, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Persephin signals through the GFRα4/RET receptor complex to promote neuronal survival, yet its recombinant production demands careful attention to the disulfide-rich cystine knot fold. This tag-free human PSPN fragment (aa 61–156) encompasses the mature, receptor-binding domain and demonstrates an ED50 below 10 ng/mL in TT medullary thyroid cancer cell proliferation assays, corresponding to a specific activity exceeding 1.0 × 10⁵ IU/mg. Such potency supports use in neuronal survival and proliferation assays, RET-dependent signaling studies, and receptor-ligand binding experiments via SPR or ELISA. Produced in *E. coli*—appropriate for this non-glycosylated growth factor—the protein meets purity greater than 97% by SDS-PAGE with endotoxin levels below 1.0 EU/μg, satisfying the quality criteria typical for cell-based bioassays and in vivo regenerative neuroscience models.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons.

Gene References into Functions

Results suggested that PSPN is a possible key regulator of oral squamous cell carcinoma (OSCC) progression via PSPN-RET-mitogen-activated protein kinase activation and that PSPN overexpression may have diagnostic potential for OSCC. PMID: 24375483
Results identify persephin, a GDNF family member, as a novel ligand for GFRalpha1/RET receptor complex. PMID: 20350599
Persephin/GFRalpha4 is unable to recruit RET protein into lipid rafts. PMID: 15225646
The results obtained suggest the involvement of NTN, PSP, and ART in processes subserving both the organization of this cortical region during development and the functional activity and maintenance of the mature human hippocampal neurons. PMID: 15829225
No differences were found in the allelic frequencies of the variants or in the haplotype distribution between Hirschsprung's disease patients & controls, nor to any demographic/clinical parameters within the group of patients. PMID: 18970938

Hide All

Subcellular Location

Secreted.

Protein Families

TGF-beta family, GDNF subfamily

Database Links

HGNC: 9579

OMIM: 602921

KEGG: hsa:5623

STRING: 9606.ENSP00000245810

UniGene: Hs.248159

Promotions