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Sample type: early morning urine samples, midstream specimen of urine, 15ml
Comments: The isolated intact exosomes had typical tea tray-like membrane structure under TEM, the average diameter of particles was 69 nm, the concentration was over 10^10 particles/mL. The result is quite good.
Sample type: Cell culture supernatant, 10ml
Comments: The protein band detected by WB was specific, the particles were intact under TEM, the typical tea tray-like structure was observed. The results are out of expectation!
Sample type: Serum, 1ml
Comments: The bands of interest were specific, I will give a like.
Store at 4℃ for short term storage or -80℃ for long term storage. It is recommended to store it after aliquoting to avoid repeated freezing and thawing.
WB validation of specific markers, transmission electron microscope (TEM), particle size (NanoFCM, NTA), etc.