Cell Supernatant Exosome Isolation Kit

Instructions
Code CSB-EI0102
Size 2T/10T
Image
  • Left: ENO1 (CSB-MA007670A0m)
    Right: GAPDH (CSB-MA000071M0m)
    1. Exosomes extracted from A375 cells
    2. A375 cell Lysate

  • Left: ENO1 (CSB-MA007670A0m)
    Right: GAPDH (CSB-MA000071M0m)
    1. Exosomes extracted from HEPG2 cells
    2. Exosomes extracted from PC-3 cells
    3. Exosomes extracted from Hela cells
    4. Exosomes extracted from U87 cells
    5. Hela cell Lysate

  • Left: ENO1 (CSB-MA007670A0m)
    Right: GAPDH (CSB-MA000071M0m)
    1. Exosomes extracted from MG63 cells
    2. Exosomes extracted from Ntera-2 cells
    3. MG63 cell Lysate

  • Left: ENO1 (CSB-MA007670A0m)
    Right: GAPDH (CSB-MA000071M0m)
    1. Exosomes extracted from Raji cells
    2. Exosomes extracted from U251 cells
    3. Raji cell Lysate

  • The exosomes extracted are complete in shape, spherical or dish-shaped, with uniform particles.

  • There are up to 3 x 1011 particles of purified exosomes per millilitre.

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Product Details

Sample Types Cell supernatant
Storage Store at 2-10°C
Shelf Life One year from the date of receipt
Description

Exosomes are a type of extracellular vesicles secreted by cells that contain a variety of RNA, proteins, and phospholipids with a diameter of about 30-150 nm. Exosomes play an important role in the transfer of material and information between cells.

This exosome isolation kit provides a simple and reliable method to extract intact exosomes from the cell supernatant. Comparing with other well-known brand kits, cell supernatant exosome isolation kit provided by CUSABIO has its own features. It is suitable for the isolation of exosomes from the supernatant of a variety of cells. And the exosomes isolated by this kit are comparable to ultracentrifugation with High Purity and High Yield. Moreover, this kit is simple to use and no equipment requirement.

Exosomal vesicles isolated by this product are suitable for various downstream applications, such as electron microscopy analysis, NTA analysis, NanoFCM analysis, Western Blot, fluorescence quantitative (qPCR), and high-throughput sequencing, etc.

FAQs

  • How to preserve the purified exosome vesicles?

    Store at 4℃ for short term storage or -80℃ for long term storage. It is recommended to store it after aliquoting to avoid repeated freezing and thawing.

  • How to obtain exosome-free serum culture cells?

    a. Ultra-centrifuge the cell culture serum for 10 hours to remove exosomes from the serum (aseptic operation).

    b. Choose serum-free media for cell culture.

    c. Choose exosome-free serum for cell culture.

  • How to identify the exosomes extracted from the cells?

    WB validation of specific markers, transmission electron microscope (TEM), particle size (NanoFCM, NTA), etc.

  • What are the advantages of CUSABIO exosome isolation kit over ultracentrifugation

    a. Simple, stable and highly repeatable.

    b. No equipment requirement.

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