Exosome Isolation Kit for Cell Culture Supernatant,Serum,Plasma,Latex,Urine,Saliva

Instructions
Code CSB-EI0102
Size 2T/10T
Image
  • CD9 (CSB-MA004969A0m)
    1. Exosomes extracted from plasma
    2. Exosomes extracted from serum
    3. Exosomes extracted from urine
    4. Exosomes extracted from Hela cells
    5. Exosomes extracted from latex
    6. Exosomes extracted from saliva

  • ENO1 (CSB-MA007670A0m)
    1. Exosomes extracted from plasma
    2. Exosomes extracted from serum
    3. Exosomes extracted from urine
    4. Exosomes extracted from Hela cells
    5. Exosomes extracted from latex
    6. Exosomes extracted from saliva

  • 1. Exosomes extracted from Hela cells
    2. Exosomes extracted from serum
    3. Exosomes extracted from plasma
    4. Exosomes extracted from latex
    5. Exosomes extracted from saliva
    6. Exosomes extracted from urine

  • Left: ENO1 (CSB-MA007670A0m)
    Right: GAPDH (CSB-MA000071M0m)
    1. Exosomes extracted from HEPG2 cells
    2. Exosomes extracted from PC-3 cells
    3. Exosomes extracted from Hela cells
    4. Exosomes extracted from U87 cells
    5. Hela cell Lysate

  • Left: ENO1 (CSB-MA007670A0m)
    Right: GAPDH (CSB-MA000071M0m)
    1. Exosomes extracted from MG63 cells
    2. Exosomes extracted from Ntera-2 cells
    3. MG63 cell Lysate

  • Left: ENO1 (CSB-MA007670A0m)
    Right: GAPDH (CSB-MA000071M0m)
    1. Exosomes extracted from Raji cells
    2. Exosomes extracted from U251 cells
    3. Raji cell Lysate

  • The exosomes extracted are complete in shape, spherical or dish-shaped, with uniform particles.

  • There are up to 3 x 1011 particles of purified exosomes per millilitre.

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Product Details

Sample Types Cell Culture Supernatant, Serum, Plasma, Latex, Urine, Saliva
Storage Store at 2-10°C
Shelf Life One year from the date of receipt
Description

Exosomes are a type of extracellular vesicles secreted by cells that contain a variety of RNA, proteins, and phospholipids with a diameter of about 30-150 nm. Exosomes play an important role in the transfer of material and information between cells.

This exosome isolation kit provides a simple and reliable method to extract intact exosomes from the cell culture supernatant, serum, plasma, latex, urine, saliva. Comparing with other well-known brand kits, cell supernatant exosome isolation kit provided by CUSABIO has its own features. It is suitable for the isolation of exosomes from the supernatant of a variety of cells. And the exosomes isolated by this kit are comparable to ultracentrifugation with High Purity and High Yield. Moreover, this kit is simple to use and no equipment requirement.

Exosomal vesicles isolated by this product are suitable for various downstream applications, such as electron microscopy analysis, NTA analysis, NanoFCM analysis, Western Blot, fluorescence quantitative (qPCR), and high-throughput sequencing, etc.

Product Review

Average Rating:
5.0 - 3 reviews

Application: TEM/NTA

Sample type: early morning urine samples, midstream specimen of urine, 15ml

Comments: The isolated intact exosomes had typical tea tray-like membrane structure under TEM, the average diameter of particles was 69 nm, the concentration was over 10^10 particles/mL. The result is quite good.

By Anonymous

Application: WB/TEM

Sample type: Cell culture supernatant, 10ml

Comments: The protein band detected by WB was specific, the particles were intact under TEM, the typical tea tray-like structure was observed. The results are out of expectation!

By Anonymous

Application: WB/TEM

Sample type: Serum, 1ml

Comments: The bands of interest were specific, I will give a like.

By Anonymous

FAQs

  • How to preserve the purified exosome vesicles?

    Store at 4℃ for short term storage or -80℃ for long term storage. It is recommended to store it after aliquoting to avoid repeated freezing and thawing.

  • How to obtain exosome-free serum culture cells?

    a. Ultra-centrifuge the cell culture serum for 10 hours to remove exosomes from the serum (aseptic operation).

    b. Choose serum-free media for cell culture.

    c. Choose exosome-free serum for cell culture.

  • How to identify the exosomes extracted from the cells?

    WB validation of specific markers, transmission electron microscope (TEM), particle size (NanoFCM, NTA), etc.

  • What are the advantages of CUSABIO exosome isolation kit over ultracentrifugation

    a. Simple, stable and highly repeatable.

    b. No equipment requirement.

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