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Exosomes are a type of extracellular vesicles secreted by cells that contain a variety of RNA, proteins, and phospholipids with a diameter of about 30-150 nm. Exosomes play an important role in the transfer of material and information between cells.
This exosome isolation kit provides a simple and reliable method to extract intact exosomes from the cell culture supernatant, serum, plasma, Breast Milk, urine, saliva, Yeast, Plant. Comparing with other well-known brand kits, cell supernatant exosome isolation kit provided by CUSABIO has its own features. It is suitable for the isolation of exosomes from the supernatant of a variety of cells. And the exosomes isolated by this kit are comparable to ultracentrifugation with High Purity and High Yield. Moreover, this kit is simple to use and no equipment requirement.
Exosomal vesicles isolated by this product are suitable for various downstream applications, such as electron microscopy analysis, NTA analysis, NanoFCM analysis, Western Blot, fluorescence quantitative (qPCR), and high-throughput sequencing, etc.
Sample type: early morning urine samples, midstream specimen of urine, 15ml
Comments: The isolated intact exosomes had typical tea tray-like membrane structure under TEM, the average diameter of particles was 69 nm, the concentration was over 10^10 particles/mL. The result is quite good.
Sample type: Cell culture supernatant, 10ml
Comments: The protein band detected by WB was specific, the particles were intact under TEM, the typical tea tray-like structure was observed. The results are out of expectation!
Sample type: Serum, 1ml
Comments: The bands of interest were specific, I will give a like.
Store at 4℃ for short term storage or -80℃ for long term storage. It is recommended to store it after aliquoting to avoid repeated freezing and thawing.
WB validation of specific markers, transmission electron microscope (TEM), particle size (NanoFCM, NTA), etc.