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ENO1 Monoclonal Antibody

Datasheet
Code CSB-MA007670A0m
Size US$350Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: K562 whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain tissue, Mouse Brain tissue, Rabbit Skeletal Muscle tissue, Rat Kidney tissue, Rabbit Kidney tissue
    All lanes ENO1 antibody at 1:10000
    Secondary
    Goat polyclonal to mouse IgG at 1/10000 dilution
    Predicted band size: 47 KDa
    Observed band size: 47 KDa
    Exposure time: 1min
  • Western Blot
    Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate
    All lanes ENO1 antibody at 1:10000
    Secondary
    Goat polyclonal to mouse IgG at 1/10000 dilution
    Predicted band size: 47 KDa
    Observed band size: 47 KDa
    Exposure time: 10s 
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg
    All lanes:  ENO1 antibody at 1:5000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 47 kDa
    Observed band size: 47 KDa
    Exposure time: 10s
  • Western Blot
    Positive WB detected in: MCF-7 whole cell lysate
    All lanes: ENO1 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
    Secondary
    Goat polyclonal to Mouse IgG at 1/10000 dilution
    Predicted band size: 47 kDa
    Observed band size: 47 KDa
    Exposure time: 10s
  • IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human liver cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human liver cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human colon cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human colon cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human pancreas tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human pancreas tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of MCF-7 cells with CSB-MA007670A0m at 1:130, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
  • Immunofluorescence staining of Hela cells with CSB-MA007670A0m at 1:130, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
  • Immunofluorescence staining of HepG2 cells with CSB-MA007670A0m at 1:130, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
  • Overlay histogram showing Hela cells stained with CSB-MA007670A0m (red line) at 1:260. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
  • Overlay histogram showing MCF-7 cells stained with CSB-MA007670A0m (red line) at 1:260. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
  • Immunoprecipitating ENO1 in HepG2 whole cell lysate
    Lane 1: Mouse control IgG (1µg) instead of CSB-MA007670A0m in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-MA007670A1m (2µl) + HepG2 whole cell lysate (500µg)
    Lane 3: HepG2 whole cell lysate (10µg)
  • 1. Exosomes extracted from plasma
    2. Exosomes extracted from serum
    3. Exosomes extracted from urine
    4. Exosomes extracted from Hela cells
    5. Exosomes extracted from latex
    6. Exosomes extracted from saliva
  • 1.Exosomes extracted from MG63 cells
    2. Exosomes extracted from Ntera-2 cells
    3. MG63 cell Lysate
  • 1.Exosomes extracted from HEPG2 cells
    2. Exosomes extracted from PC-3 cells
    3. Exosomes extracted from Hela cells
    4. Exosomes extracted from U87 cells
    5. Hela cell Lysate
  • 1. Exosomes extracted from Raji cells
    2. Exosomes extracted from U251 cells
    3. Raji cell Lysate
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Product Details

Description

CUSABIO immunized the mouse with the recombinant human ENO1 protein (2-434AA) to get the splenocytes that secret the ENO1 antibody. The ENO1-producing splenocytes were subsequently fused with myeloma cells to form hybridomas, which were inoculated into the abdominal cavity of mice. The mouse ascites was collected and purified to get the monoclonal anti-ENO1 antibody. Following purification through protein G, the purity of the monoclonal ENO1 antibody is more than 95%. It is matched with the mouse IgG1 isotype. And it targets ENO1 in ELISA, WB, IHC, IP, IF, and FC applications and reacts with human, mouse, rabbit, and rat samples.

ENO1 mainly functions to catalyze glycolysis in the cytoplasm of prokaryotic and eukaryotic cells. Differential expression of ENO1 has been associated with several pathologies, such as cancer, Alzheimer's disease, and rheumatoid arthritis.
Full Product Name Mouse anti-Homo sapiens (Human) ENO1 Monoclonal antibody
Uniprot No. P06733
Target Names ENO1
Alternative Names Alpha-enolase (2-phospho-D-glycerate hydro-lyase) (C-myc promoter-binding protein) (Enolase 1) (MBP-1) (MPB-1) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (Plasminogen-binding protein), ENO1, ENO1L1, MBPB1, MPB1
Raised in Mouse
Species Reactivity Human, Mouse, Rat, Rabbit
Immunogen Recombinant Human Alpha-enolase protein (2-434AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG1
Clone No. 8H9G12
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF, FC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:5000-1:320000
IHC 1:200-1:500
IF 1:50-1:200
FC 1:100-1:300
IP 2μl-4μl
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
(From Uniprot)
Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.; MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Gene References into Functions
  1. Our data provide strong evidence that alpha-enolase short hairpin RNA interference vector can effectively suppress the proliferation and increase chemosensitivity of MKN45 cells, which may provide a novel gene therapy for gastric cancer. PMID: 29986635
  2. Elevated ENO1 could promote the proliferation of retinoblastoma cells. PMID: 29913409
  3. Together these findings suggest a possible mechanism of host invasion by HIV-1 through the CAWLEAQ motif of Nef-mediated regulation of ENO1 and identify a potential therapeutic target for HIV-1 entry at mucosal barriers. PMID: 29404888
  4. We propose that ENO1 is a useful indicator of parakeratosis and might have a potential role in cellular TJ barrier function in the epidermis PMID: 29430682
  5. The authors found that ENO1 expression was upregulated in the hepatitis B virus-infected liver tissues and cells. Silencing of ENO1 resulted in a significant reduction in hepatitis B virus replication, and this siRNA-mediated reaction also caused the upregulation of expression of type I interferon and downstream interferon-stimulated genes. PMID: 29080231
  6. serum anti-alpha-enolase antibody levels positively correlated with serum whole IgG and 24-hour urine protein and negatively correlated with serum D-dimer level in patients with systemic lupus erythematosus PMID: 28728510
  7. ENO1 is a crucial factor promoting neoplastic transformation exclusively in terminal respiratory unit (TRU) subtype lung adenocarcinomas (ADCs). PMID: 29090499
  8. Protein arginine methyltransferase 5 (PRMT5) was identified to be responsible for Eno-1 methylation. Overexpression of PRMT5 and caveolin-1 enhanced levels of membrane-bound extracellular Eno-1 and, conversely, pharmacological inhibition of PRMT5 attenuated Eno-1 cell-surface localization. PMID: 29501774
  9. Our results suggested that alpha-enolase level was significantly elevated in pancreatic cancer tissues, which was closely associated with pancreatic cancer progression PMID: 28824297
  10. ENO1 is a novel biomarker to predict drug resistance and overall prognosis in gastric cancer. PMID: 28548950
  11. ENO1 can elicit humoral immune response in NSCLC and its autoantibody has association with the tumorigenesis of NSCLC. Furthermore, these intriguing results suggest the possibility of autoantibody against ENO1 serving as a potential diagnostic biomarker in NSCLC and have implications for defining novel histological determinants of NSCLC. PMID: 28456790
  12. The study provides solid evidence that there is the interaction between GRN A and ENO1 and the interaction is responsible for the effects of GRN A on glucose uptake as well as cancer cell migration and invasion. PMID: 28415822
  13. Our findings showed that both S100beta and NSE levels similarly increased during CPB and immediately after CPB during sevoflurane and propofol based anesthesia. PMID: 26856295
  14. ENO1 promotes in pancreatic ductal adenocarcinoma (PDA) cells cell survival, migration, and metastasis through cooperation with integrins and uPAR PMID: 28086938
  15. ENO1 is a potent promoter of colorectal cancer genesis and metastasis at least in part though regulating AMPK/mTOR pathway. PMID: 27996156
  16. comparing the T cell receptor repertoire of ENO1-specific peripheral and infiltrating tumor T cells from the same patient suggests that ENO1-specific T cells, despite having a different functional profile, can recirculate from the tumor to the periphery. PMID: 27210467
  17. ENO1 is able to activate in vitro the CD14-dependent TLR4 pathway on monocytes involving a dual mechanism firstly pro-inflammatory and secondly anti-inflammatory. PMID: 27025255
  18. High ENO1 expression is associated with cancer. PMID: 26734996
  19. An immune-modulatory role has been proposed for surface alpha-enolase and our findings of decreased expression suggest that deficits in surface alpha-enolase merit investigation in the context of immune dysfunction during ageing and vascular disease PMID: 26432922
  20. Systems biology approach reveals possible evolutionarily conserved moonlighting functions for enolase PMID: 25978602
  21. ENO1 was demonstrated to be up-regulated by HIF-1alpha in retinal pigment epithelial cells in response to hypoxia, without influencing VEGF secretion. PMID: 26882120
  22. In this study, ENO1 silencing significantly reduced cell glycolysis, proliferation, migration, and invasion in vitro, as well as tumorigenesis and metastasis in vivo by modulating p85 suppression. PMID: 25951350
  23. alpha-Enolase increases after injury and may activate pulmonary endothelial cells and prime PMNs through plasmin activity and PAR-2 activation PMID: 25944790
  24. High ENO1 expression is associated with invasiveness of pancreatic cancer. PMID: 25860938
  25. tRK1 forms a complex with human enolases and interacts with tRK1 and human pre-lysyl-tRNA synthetase (preKARS2) PMID: 25918939
  26. The association of ENO1 and GPI with postthaw sperm viability and motility was confirmed using Pearson's linear correlation. ENO1 and GPI can be used as markers of human sperm freezability before starting the cryopreservation procedure. PMID: 25910678
  27. This study showed that ENO1 is responsible for non-small cell lung cancer proliferation and metastasis PMID: 25887760
  28. ENO1 induces gene expression, lactate production, cell proliferation and migration and can be negatively regulated by FBXW7 at the posttranslational level. PMID: 26097998
  29. alpha-enolase binds plasminogen and modulates its activation, it is plausible to speculate the association of the increase in alpha-enolase secretion by infected hepatic cells with the haemostatic dysfunction observed in dengue patients PMID: 25171719
  30. Data suggest that endoplasmic reticulum stress up-regulates ENO1/MBP1 expression through the AKT/PERK/eIF2a signaling axis in both breast cancer cells and non-tumor cells. PMID: 26144282
  31. Low ENO1 expression is associated with clear cell renal cell carcinoma. PMID: 26037892
  32. ENO1 is an independent prognostic factor, significantly correlated with overall survival of Non-Hodgkin's Lymphoma patients. PMID: 26024773
  33. STIM1/ORAI1-mediated Ca2+ influx regulates enolase-1 exteriorization in cancer cells. PMID: 25805497
  34. the results indicated that the combination of ENO1 and CYPA can serve as a potential molecular marker for the early detection of NPC. PMID: 24998431
  35. Using shotgun proteomics together with western blotting and ELISA, we identify sputum ENO1 as a biomarker candidate for lung cancer PMID: 24984566
  36. Our data suggest that ENO1 was upregulated by CagA protein thereby providing a novel mechanism underlying H. pylori-mediated gastric diseases. PMID: 24841372
  37. These data show a multiantibody composition in lupus nephritis, where IgG2 autoantibodies against alpha-enolase and annexin AI predominate in the glomerulus and can be detected in serum. PMID: 24790181
  38. Isotypes of anti-citrullinated fibrinogen and alpha-enolase can be found in the serum of children with juvenile idiopathic arthritis of all onset types. PMID: 25068682
  39. Overexpression of ENO1 is associated with glioma progression. PMID: 24650096
  40. Citrullination of ENO1 is associated with neoplasms. PMID: 24099319
  41. the differential association of ENO-1 with caveolar proteins regulates ENO-1 subcellular localization and, consequently, ENO-1-dependent cell migration and invasion. PMID: 24628430
  42. ENO1 overexpression can inhibit EMT in vitro by suppressing ERK1/2 phosphorylation PMID: 23676977
  43. surface alpha-enolase promotes extracellular matrix degradation and invasion of cancer cells and that targeting surface alpha-enolase is a promising approach to suppress tumor metastasis. PMID: 23894455
  44. plasma levels are significantly higher in renal cell carcinoma patients than in controls PMID: 23113677
  45. results indicate that Streptococcus sanguinis infection and the sera of Behcet's disease patients with active disease are inflammatory stimuli that can induce membranous alpha-enolase expression in endothelial cells PMID: 23131860
  46. The reduction in enolase-1 expression significantly decreases the response to hypoxia and enhances the sensitivity of the cells to radiation therapy. PMID: 23381546
  47. ENO1 is a passenger locus homozygously deleted as part of the 1p36 locus in glioblastoma and inhibition of ENO2 is selectively lethal to ENO1 deleted tumor cells, but not normal non-cancerous cells. PMID: 22895339
  48. These results extend, confirm, and generalize the evidence supporting the specificity of the anti-CEP-1 antibody-positive subgroup of patients with rheumatoid arthritis among anti-CCP antibody-positive patients with RA. PMID: 21360494
  49. Data show six differentially expressed proteins were identified as HSP70, PPIA and alpha-Enolase (up-regulated) S100-A9, PIMT and beta-5 tubulin (down-regulated), most of which had been shown to play a potential role in the pathogenesis of atherosclerosis. PMID: 21839816
  50. results indicate that C-peptide has the capacity to activate alpha-enolase through a specific interaction between E27 of the peptide and K434 of the enzyme PMID: 22577163

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Subcellular Location Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.; [Isoform MBP-1]: Nucleus.
Protein Families Enolase family
Tissue Specificity The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Database Links

HGNC: 3350

OMIM: 172430

KEGG: hsa:2023

STRING: 9606.ENSP00000234590

UniGene: Hs.517145
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