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ENO1 Monoclonal Antibody

Datasheet

Code	CSB-MA007670A0m
Size	US$350
Image	Western Blot Positive WB detected in: K562 whole cell lysate, NIH/3T3 whole cell lysate, Rat Brain tissue, Mouse Brain tissue, Rabbit Skeletal Muscle tissue, Rat Kidney tissue, Rabbit Kidney tissue All lanes ENO1 antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 1/10000 dilution Predicted band size: 47 KDa Observed band size: 47 KDa Exposure time: 1min Western Blot Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate All lanes ENO1 antibody at 1:10000 Secondary Goat polyclonal to mouse IgG at 1/10000 dilution Predicted band size: 47 KDa Observed band size: 47 KDa Exposure time: 10s Western Blot Positive WB detected in: HepG2 whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg All lanes: ENO1 antibody at 1:5000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 47 kDa Observed band size: 47 KDa Exposure time: 10s Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: ENO1 antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 47 kDa Observed band size: 47 KDa Exposure time: 10s IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human liver cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human liver cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human colon cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human colon cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human pancreas tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-MA007670A0m diluted at 1:500 and staining in paraffin-embedded human pancreas tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of MCF-7 cells with CSB-MA007670A0m at 1:130, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Immunofluorescence staining of Hela cells with CSB-MA007670A0m at 1:130, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). Overlay histogram showing Hela cells stained with CSB-MA007670A0m (red line) at 1:260. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. Overlay histogram showing MCF-7 cells stained with CSB-MA007670A0m (red line) at 1:260. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed. Immunoprecipitating ENO1 in HepG2 whole cell lysate Lane 1: Mouse control IgG (1µg) instead of CSB-MA007670A0m in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000) Lane 2: CSB-MA007670A1m (2µl) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (10µg)
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Product Details
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Target Data

Product Details

Full Product Name

Mouse anti-Human ENO1 Monoclonal antibody

Uniprot No.

P06733

Target Names

ENO1

Alternative Names

Alpha-enolase (2-phospho-D-glycerate hydro-lyase) (C-myc promoter-binding protein) (Enolase 1) (MBP-1) (MPB-1) (Non-neural enolase) ( NNE) (Phosphopyruvate hydratase) (Plasminogen-binding protein), ENO1, ENO1L1, MBPB1, MPB1

Raised in

Mouse

Species Reactivity

Human, Mouse, Rat, Rabbit

Immunogen

Recombinant Human Alpha-enolase protein (2-434AA)

Immunogen Species

Human

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

IgG1

Clone No.

8H9G12

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:5000-1:320000
IHC	1:200-1:500
IF	1:50-1:200
FC	1:100-1:300
IP	2μl-4μl

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function	Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.; FUNCTION
Gene References into Functions	Our data provide strong evidence that alpha-enolase short hairpin RNA interference vector can effectively suppress the proliferation and increase chemosensitivity of MKN45 cells, which may provide a novel gene therapy for gastric cancer. PMID: 29986635 Elevated ENO1 could promote the proliferation of retinoblastoma cells. PMID: 29913409 Together these findings suggest a possible mechanism of host invasion by HIV-1 through the CAWLEAQ motif of Nef-mediated regulation of ENO1 and identify a potential therapeutic target for HIV-1 entry at mucosal barriers. PMID: 29404888 We propose that ENO1 is a useful indicator of parakeratosis and might have a potential role in cellular TJ barrier function in the epidermis PMID: 29430682 The authors found that ENO1 expression was upregulated in the hepatitis B virus-infected liver tissues and cells. Silencing of ENO1 resulted in a significant reduction in hepatitis B virus replication, and this siRNA-mediated reaction also caused the upregulation of expression of type I interferon and downstream interferon-stimulated genes. PMID: 29080231 serum anti-alpha-enolase antibody levels positively correlated with serum whole IgG and 24-hour urine protein and negatively correlated with serum D-dimer level in patients with systemic lupus erythematosus PMID: 28728510 ENO1 is a crucial factor promoting neoplastic transformation exclusively in terminal respiratory unit (TRU) subtype lung adenocarcinomas (ADCs). PMID: 29090499 Protein arginine methyltransferase 5 (PRMT5) was identified to be responsible for Eno-1 methylation. Overexpression of PRMT5 and caveolin-1 enhanced levels of membrane-bound extracellular Eno-1 and, conversely, pharmacological inhibition of PRMT5 attenuated Eno-1 cell-surface localization. PMID: 29501774 Our results suggested that alpha-enolase level was significantly elevated in pancreatic cancer tissues, which was closely associated with pancreatic cancer progression PMID: 28824297 ENO1 is a novel biomarker to predict drug resistance and overall prognosis in gastric cancer. PMID: 28548950 ENO1 can elicit humoral immune response in NSCLC and its autoantibody has association with the tumorigenesis of NSCLC. Furthermore, these intriguing results suggest the possibility of autoantibody against ENO1 serving as a potential diagnostic biomarker in NSCLC and have implications for defining novel histological determinants of NSCLC. PMID: 28456790 The study provides solid evidence that there is the interaction between GRN A and ENO1 and the interaction is responsible for the effects of GRN A on glucose uptake as well as cancer cell migration and invasion. PMID: 28415822 Our findings showed that both S100beta and NSE levels similarly increased during CPB and immediately after CPB during sevoflurane and propofol based anesthesia. PMID: 26856295 ENO1 promotes in pancreatic ductal adenocarcinoma (PDA) cells cell survival, migration, and metastasis through cooperation with integrins and uPAR PMID: 28086938 ENO1 is a potent promoter of colorectal cancer genesis and metastasis at least in part though regulating AMPK/mTOR pathway. PMID: 27996156 comparing the T cell receptor repertoire of ENO1-specific peripheral and infiltrating tumor T cells from the same patient suggests that ENO1-specific T cells, despite having a different functional profile, can recirculate from the tumor to the periphery. PMID: 27210467 ENO1 is able to activate in vitro the CD14-dependent TLR4 pathway on monocytes involving a dual mechanism firstly pro-inflammatory and secondly anti-inflammatory. PMID: 27025255 High ENO1 expression is associated with cancer. PMID: 26734996 An immune-modulatory role has been proposed for surface alpha-enolase and our findings of decreased expression suggest that deficits in surface alpha-enolase merit investigation in the context of immune dysfunction during ageing and vascular disease PMID: 26432922 Systems biology approach reveals possible evolutionarily conserved moonlighting functions for enolase PMID: 25978602 ENO1 was demonstrated to be up-regulated by HIF-1alpha in retinal pigment epithelial cells in response to hypoxia, without influencing VEGF secretion. PMID: 26882120 In this study, ENO1 silencing significantly reduced cell glycolysis, proliferation, migration, and invasion in vitro, as well as tumorigenesis and metastasis in vivo by modulating p85 suppression. PMID: 25951350 alpha-Enolase increases after injury and may activate pulmonary endothelial cells and prime PMNs through plasmin activity and PAR-2 activation PMID: 25944790 High ENO1 expression is associated with invasiveness of pancreatic cancer. PMID: 25860938 tRK1 forms a complex with human enolases and interacts with tRK1 and human pre-lysyl-tRNA synthetase (preKARS2) PMID: 25918939 The association of ENO1 and GPI with postthaw sperm viability and motility was confirmed using Pearson's linear correlation. ENO1 and GPI can be used as markers of human sperm freezability before starting the cryopreservation procedure. PMID: 25910678 This study showed that ENO1 is responsible for non-small cell lung cancer proliferation and metastasis PMID: 25887760 ENO1 induces gene expression, lactate production, cell proliferation and migration and can be negatively regulated by FBXW7 at the posttranslational level. PMID: 26097998 alpha-enolase binds plasminogen and modulates its activation, it is plausible to speculate the association of the increase in alpha-enolase secretion by infected hepatic cells with the haemostatic dysfunction observed in dengue patients PMID: 25171719 Data suggest that endoplasmic reticulum stress up-regulates ENO1/MBP1 expression through the AKT/PERK/eIF2a signaling axis in both breast cancer cells and non-tumor cells. PMID: 26144282 Low ENO1 expression is associated with clear cell renal cell carcinoma. PMID: 26037892 ENO1 is an independent prognostic factor, significantly correlated with overall survival of Non-Hodgkin's Lymphoma patients. PMID: 26024773 STIM1/ORAI1-mediated Ca2+ influx regulates enolase-1 exteriorization in cancer cells. PMID: 25805497 the results indicated that the combination of ENO1 and CYPA can serve as a potential molecular marker for the early detection of NPC. PMID: 24998431 Using shotgun proteomics together with western blotting and ELISA, we identify sputum ENO1 as a biomarker candidate for lung cancer PMID: 24984566 Our data suggest that ENO1 was upregulated by CagA protein thereby providing a novel mechanism underlying H. pylori-mediated gastric diseases. PMID: 24841372 These data show a multiantibody composition in lupus nephritis, where IgG2 autoantibodies against alpha-enolase and annexin AI predominate in the glomerulus and can be detected in serum. PMID: 24790181 Isotypes of anti-citrullinated fibrinogen and alpha-enolase can be found in the serum of children with juvenile idiopathic arthritis of all onset types. PMID: 25068682 Overexpression of ENO1 is associated with glioma progression. PMID: 24650096 Citrullination of ENO1 is associated with neoplasms. PMID: 24099319 the differential association of ENO-1 with caveolar proteins regulates ENO-1 subcellular localization and, consequently, ENO-1-dependent cell migration and invasion. PMID: 24628430 ENO1 overexpression can inhibit EMT in vitro by suppressing ERK1/2 phosphorylation PMID: 23676977 surface alpha-enolase promotes extracellular matrix degradation and invasion of cancer cells and that targeting surface alpha-enolase is a promising approach to suppress tumor metastasis. PMID: 23894455 plasma levels are significantly higher in renal cell carcinoma patients than in controls PMID: 23113677 results indicate that Streptococcus sanguinis infection and the sera of Behcet's disease patients with active disease are inflammatory stimuli that can induce membranous alpha-enolase expression in endothelial cells PMID: 23131860 The reduction in enolase-1 expression significantly decreases the response to hypoxia and enhances the sensitivity of the cells to radiation therapy. PMID: 23381546 ENO1 is a passenger locus homozygously deleted as part of the 1p36 locus in glioblastoma and inhibition of ENO2 is selectively lethal to ENO1 deleted tumor cells, but not normal non-cancerous cells. PMID: 22895339 These results extend, confirm, and generalize the evidence supporting the specificity of the anti-CEP-1 antibody-positive subgroup of patients with rheumatoid arthritis among anti-CCP antibody-positive patients with RA. PMID: 21360494 Data show six differentially expressed proteins were identified as HSP70, PPIA and alpha-Enolase (up-regulated) S100-A9, PIMT and beta-5 tubulin (down-regulated), most of which had been shown to play a potential role in the pathogenesis of atherosclerosis. PMID: 21839816 results indicate that C-peptide has the capacity to activate alpha-enolase through a specific interaction between E27 of the peptide and K434 of the enzyme PMID: 22577163 ENO1-positive monocytes and macrophages are found in the inflamed synovium from rheumatoid arthritis patients. PMID: 22623332 Increased citrullination of Arg9 in ENO1 is associated with Creutzfeldt-Jakob disease and Alzheimer's disease. PMID: 22551201 Data show that ENOA, PARK7 and Beta-actin are proper reference standards in obesity studies based on omental fat. PMID: 22272336 ENO1 over expression may play a important role in cell proliferation and progression of nasopharyngeal cancer. PMID: 21950004 Silencing of ENO1 resulted in growth inhibition and cell cycle arrest of gastric cancer cells. PMID: 21751384 glomerular alpha-enolase is a target antigen of autoimmunity in human MGN. Circulating anti alpha-enolase auto-antibodies can be detected in sera of a significant quota of membranous glomerulonephritis patients. PMID: 21640210 The elevated levels of ENO1 protein in the tumor tissues and the plasma samples from NSCLC patients indicate ENO1 may be a candidate biomarker of lung cancer. PMID: 21159241 Inhibition of ENO-1 expression significantly augmented 4-OHT cytotoxicity in tamoxifen-resistant breast cancer cells. PMID: 19655245 Loss of nuclear MBP-1 expression is associated with the progression of invasive ductal breast carcinoma. PMID: 20886042 Overexpression of ENO1 is associated with recurrences in chordomas. PMID: 20721957 novel transcript was alternatively transcribed from intron III of the ENO1 gene and was feasible for MBP-1 production PMID: 20849415 a prognostic role of ENO1 overexpression was demonstrated in head and neck cancer and ENO1-mediated promotion of cell transformation and invasion partly via induced CCL20 expression. PMID: 20435467 SEDLIN is present in the nucleus, forms homodimers and that SEDT-associated mutations cause a loss of interaction with the transcription factors MBP1, PITX1 and SF1. PMID: 20498720 Association of upregulated ACLY and ENO1 expression by chi square for all probe sets (reporters) combined and correlation for numbers of probe sets indicating shared upregulation of these genes. PMID: 19795461 These results suggest that MBP-1-suppressed COX-2 expression plays an important role in the inhibition of growth and progression of gastric cancer. PMID: 19846662 Proteomic analysis of human brain identifies alpha-enolase as a novel autoantigen in Hashimoto's encephalopathy. PMID: 12297304 A target of excess protein nitration in Alzheimer disease (AD) brain, providing evidence of the importance of oxidative stress in AD progression PMID: 12787059 The alpha-enolase protein is the target protein of serum anti-endothelial antibody in Behcet's disease patients. PMID: 12847697 antibodies against human alpha-enolase target antigens in retinal ganglion cells and inner nuclear layer cells and induce the apoptotic death of sensitive cells in rat retinal explants PMID: 15324934 Results suggest a novel role of c-myc promoter-binding protein 1 for suppression of prostate cancer cell growth by regulating the MEK5-mediated signaling pathway. PMID: 15805119 ENO1 has tumour suppressor activity and high level of ENO1 expression has growth inhibitory effects PMID: 16359544 Depletion of MBP-1 in prostate cancer cells perturbs cell proliferation by inhibiting cyclin A and cyclin B1 expression. PMID: 16762917 Our results indicate that differential subcellular localization of ENO1 products may be closely related to carcinogenesis of the oral epithelium PMID: 17284257 These findings further support the distinct roles of alpha-enolase and its MBP-1 variant in maintaining cell homeostasis. Moreover, these data suggest a novel function for NS1-BP in the control of cell proliferation. PMID: 17996313 Enolase on the surface of cells may be a receptor for plasminogen. The enolase/plasminogen (plasmin) system is one of the mechanisms facilitating the invasiveness of pathogens and it plays a role in myogenesis and in the development of tumor tissues. PMID: 18033204 TGF-beta1-induced growth arrest was associated with notable downregulation of the myc-binding protein-1 (MBP-1). PMID: 18070418 These results indicate that the activated Notch1 receptor and alpha-enolase or MBP-1 cooperate in controlling c-myc expression through binding the YY1 response element of the c-myc promoter to regulate tumorigenesis. PMID: 18490439 Crystal structure of ENO1 is presented at 2.2 A resolution. Despite its high sequence similarity to other enolases, the ENO1 structure exhibits distinct surface properties, explaining its various activities, including plasmin(ogen) and DNA binding. PMID: 18560153 Alpha-enolase was citrullinated in rheumatoid arthritis synovial fluid. PMID: 18668562 HIF-1alpha-regulated glycolysis module is closely related to the aggressive phenotype of hepatocellular carcinoma, and ENO1, a glycolysis module gene, might have a role in preventing metastasis PMID: 18813785 An important mechanism of inflammatory cell invasion mediated by increased cell-surface expression of ENO-1. PMID: 19182206 Crystals of alpha-Enolase from human liver were obtained by the hanging-drop vapour-diffusion method and diffracted to 2.5 A resolution. PMID: 19255486 fatty acid-binding protein-5, squamous cell carcinoma antigens 2, alpha-enolase, annexin II, apolipoprotein A-I and albumin were detected at a high level in Atopic dermatitis skin lesions, but scarcely in the normal controls PMID: 19339807 Results indicate that alpha-enolase elicits a PDAC-specific, integrated humoral and cellular response (proliferative response by T-cells). PMID: 19425054 alpha-enolase and MBP-1 associate with the kelch protein NS1-BP. c-Myc gene transcriptional repression exerted by MBP-1 is enhanced by NS1-BP. PMID: 17996313 Show More Hide All
Subcellular Location	Cytoplasm, Cell membrane, Cytoplasm, myofibril, sarcomere, M line
Protein Families	Enolase family
Tissue Specificity	The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Database Links	HGNC: 3350 OMIM: 172430 KEGG: hsa:2023 STRING: 9606.ENSP00000234590 UniGene: Hs.517145