Code | CSB-MA007670A0m |
Size |
US$350Purchase it in Cusabio online store (only available for customers from the US) |
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Description |
CUSABIO immunized the mouse with the recombinant human ENO1 protein (2-434AA) to get the splenocytes that secret the ENO1 antibody. The ENO1-producing splenocytes were subsequently fused with myeloma cells to form hybridomas, which were inoculated into the abdominal cavity of mice. The mouse ascites was collected and purified to get the monoclonal anti-ENO1 antibody. Following purification through protein G, the purity of the monoclonal ENO1 antibody is more than 95%. It is matched with the mouse IgG1 isotype. And it targets ENO1 in ELISA, WB, IHC, IP, IF, and FC applications and reacts with human, mouse, rabbit, and rat samples. ENO1 mainly functions to catalyze glycolysis in the cytoplasm of prokaryotic and eukaryotic cells. Differential expression of ENO1 has been associated with several pathologies, such as cancer, Alzheimer's disease, and rheumatoid arthritis. |
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Full Product Name | Mouse anti-Homo sapiens (Human) ENO1 Monoclonal antibody | ||||||||||||
Uniprot No. | P06733 | ||||||||||||
Target Names | ENO1 | ||||||||||||
Alternative Names | Alpha-enolase (2-phospho-D-glycerate hydro-lyase) (C-myc promoter-binding protein) (Enolase 1) (MBP-1) (MPB-1) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (Plasminogen-binding protein), ENO1, ENO1L1, MBPB1, MPB1 | ||||||||||||
Raised in | Mouse | ||||||||||||
Species Reactivity | Human, Mouse, Rat, Rabbit | ||||||||||||
Immunogen | Recombinant Human Alpha-enolase protein (2-434AA) | ||||||||||||
Immunogen Species | Homo sapiens (Human) | ||||||||||||
Conjugate | Non-conjugated | ||||||||||||
Clonality | Monoclonal | ||||||||||||
Isotype | IgG1 | ||||||||||||
Clone No. | 8H9G12 | ||||||||||||
Purification Method | >95%, Protein G purified | ||||||||||||
Concentration | It differs from different batches. Please contact us to confirm it. | ||||||||||||
Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
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Form | Liquid | ||||||||||||
Tested Applications | ELISA, WB, IHC, IF, FC, IP | ||||||||||||
Recommended Dilution |
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Protocols | ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol Immunofluorescence (IF) Protocol Flow Cytometry (FC) Protocol Immunoprecipitation (IP) Protocol |
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Troubleshooting and FAQs | Antibody FAQs | ||||||||||||
Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||||||||||
Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
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Function |
Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.; MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
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Gene References into Functions |
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Subcellular Location | Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.; [Isoform MBP-1]: Nucleus. |
Protein Families | Enolase family |
Tissue Specificity | The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. |
Database Links |
HGNC: 3350 OMIM: 172430 KEGG: hsa:2023 STRING: 9606.ENSP00000234590 UniGene: Hs.517145 |