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| Code | CSB-MA004969A0m |
| Size | US$350 |
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| Description |
This CD9 monoclonal antibody was a laboratory-produced antibody designed to recognize and bind to CD9. Here was the production process. Injected a Recombinant Human CD9 antigen protein into a mouse so that the mouse’s immune response was induced and the B lymphocytes was obtained; Fused the mouse myeloma cells and B lymphocytes to produce hybridoma cells; Selected and cultured the hybridoma cells and finally use them for the production of the CD9 monoclonal antibody, which has been validated in ELISA, WB, IHC. CD9 protein is a transmembrane glycoprotein that can regulate cell proliferation, movement and differentiation. The protein is transmembrane four times and has a molecular weight of 24KD-27KD. It consists of 227 amino acid residues. Most of them are distributed in the cell membrane, and a few are distributed in the cell plate. Pseudopodia, filopodia and cell vesicles. The protein structure is a single polypeptide chain structure, containing four highly hydrophobic transmembrane domains. The N-terminal and C-terminal are shorter amino acid sequences located in the cytoplasm; the transmembrane domains are separated by hydrophilic groups and are located in the cell The outer hydrophilic group forms two ring structures of varying sizes. The two transmembrane domains and partial amino acid sequences of the ring structure are highly conserved. Some polar amino acids in the transmembrane structure are located in the central part of the ion channel. Participating in the formation of ion channels may be related to intercellular signal transduction. The large loop structure outside the cell contains conservative CCG sequences and cysteine residue sequences, which may be related to the evolution of organisms. Small loops outside the cell The structure contains glycosylation sites. CD9 can be modified by N-glycosylation after transcription. The macrocyclic structure contains monoclonal antibody binding sites. These binding sites are closely related to the function of CD9. |
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| Full Product Name | Mouse anti-Homo sapiens (Human) CD9 Monoclonal antibody | ||||||
| Uniprot No. | P21926 | ||||||
| Target Names | CD9 | ||||||
| Alternative Names | CD9; MIC3; TSPAN29; GIG2; CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD antigen CD9 | ||||||
| Raised in | Mouse | ||||||
| Species Reactivity | Human | ||||||
| Immunogen | Recombinant Human CD9 antigen protein (112-195AA) | ||||||
| Immunogen Species | Homo sapiens (Human) | ||||||
| Conjugate | Non-conjugated | ||||||
| Clonality | Monoclonal | ||||||
| Isotype | IgG2a | ||||||
| Clone No. | 5A6D8 | ||||||
| Purification Method | >95%, Protein G purified | ||||||
| Concentration | It differs from different batches. Please contact us to confirm it. | ||||||
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
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| Form | Liquid | ||||||
| Tested Applications | ELISA, WB, IHC | ||||||
| Recommended Dilution |
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| Protocols | ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol |
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| Troubleshooting and FAQs | Antibody FAQs | ||||||
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||||
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
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| Function |
Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion. In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration. In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption. Acts as a receptor for PSG17. Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis.
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| Gene References into Functions |
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| Subcellular Location | Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein. Secreted, extracellular exosome. |
| Protein Families | Tetraspanin (TM4SF) family |
| Tissue Specificity | Detected in platelets (at protein level). Expressed by a variety of hematopoietic and epithelial cells. |
| Database Links |
HGNC: 1709 OMIM: 143030 KEGG: hsa:928 STRING: 9606.ENSP00000009180 UniGene: Hs.114286 |
