Recombinant Mouse Fibroblast growth factor 1(Fgf1) (Active)

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Code CSB-AP004151MO
Size US$1307Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 95% as determined by SDS-PAGE.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity The ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of Heparin is less than 1.6 ng/mL.
Target Names Fgf1
Uniprot No. P61148
Research Area Signal Transduction
Alternative Names Fgf1; Fgf-1; Fgfa; Fibroblast growth factor 1; FGF-1; Acidic fibroblast growth factor; aFGF; Heparin-binding growth factor 1; HBGF-1
Species Mus musculus (Mouse)
Source E.coli
Expression Region 16-155aa
Complete Sequence FNLPLGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESAGEVYIKGTETGQYLAMDTEGLLYGSQTPNEECLFLERLEENHYNTYTSKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD
Mol. Weight 15.7 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 500 mM NaCl, pH 6.6
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 5-10 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV
Gene References into Functions
  1. The present results provided evidence that aFGF accelerates the progression of atherosclerosis and suggested that aFGF may be a potential therapeutic target for the prevention of atherosclerosis development. PMID: 29845277
  2. TUG1 knockdown ameliorates atherosclerosis by modulating FGF1 via miR-133a in ApoE knockout mice. PMID: 29268138
  3. Fibroblast growth factor (FGF1 and FGF2), but not vascular endothelial growth factor (VEGF) rescued Psen1-/- cells from serum starvation induced apoptosis.In the absence of serum, FGF2 immunoreactivity was distributed diffusely in cytoplasmic and nuclear vesicles of wt and Psen1-/- cells, as levels of FGF2 in nuclear and cytosolic fractions were not significantly different. PMID: 27443835
  4. Suboptimal FGFR activation by a weak FGF1-FGFR dimer is sufficient to evoke a metabolic response, whereas full FGFR activation by stable and sustained dimerization is required to elicit a mitogenic response. PMID: 28813681
  5. FGF1 protects neuroblastoma cells from p53-dependent apoptosis through an intracrine pathway regulated by FGF1 phosphorylation. PMID: 29048426
  6. the HDAC3-N-CoR corepressor complex leaves the Fgf1b promoter and a complex involving the translocated CRTC1, phosphorylated CREB, and histone acetyltransferase CBP induces transient transcription. PMID: 28076781
  7. lncRNA-Map2k4 is the target gene of miR-199a, and its down-regulation promotes miR-199a expression in neurons. miR-199a targeted regulation of FGF1 expression in neurons. PMID: 28655615
  8. clearly demonstrate the different specificity of FGF12-FGFR1c2 and FGF22-FGFR1c2 for well defined HS structures and suggest that it is now possible to chemoenzymatically synthesize precise HS polysaccharides that can selectively mediate growth factor signaling PMID: 28031461
  9. The study supports a pro-adipogenic role for betaKlotho in skeletal muscle fibro/adipogenesis and calls for further research on involvement of the FGF-FGFR-betaKlotho axis in the fibro/adipogenic infiltration associated with functional deterioration of skeletal muscle in aging and muscular dystrophy. PMID: 26881702
  10. fibroblast growth factor 1 (FGF1) to be synergistically induced by heat shock and wounding. PMID: 27638903
  11. Data suggest that Fgf1-mediated signaling represents an important signaling cascade related to adipogenesis and visceral adiposity; expression of Fgf1 (fibroblast growth factor 1) and Fgfr1 (fibroblast growth factor receptor 1) is up-regulated in adipose tissue of obese mice (both obese mice due to high-fat diet and obese mice due to genetic deletion of leptin). PMID: 26847131
  12. These results suggest that FGFs promote hair growth by inducing the anagen phase in resting hair follicles and might be a potential hair growth-promoting agent. PMID: 25685806
  13. Study presents a transcript profiling of remyelinated multiple sclerosis lesions and identified FGF1 as a promoter of remyelination PMID: 25589163
  14. Nucleolin-FGF1 interaction is critical for the intranuclear phosphorylation of FGF1 by PKCdelta and thereby the regulation of nuclear export of FGF1. PMID: 24595027
  15. parenteral delivery of a single dose of recombinant FGF1 (rFGF1) results in potent, insulin-dependent lowering of glucose levels in diabetic mice that is dose-dependent but does not lead to hypoglycaemia PMID: 25043058
  16. Although FGF1 transgenic mice had a normal phenotype with unperturbed kidney structure, they showed a severely inhibited kidney repair after unilateral ischemia/reperfusion. This was manifested by a strong decrease of postischemic kidney size and weight. PMID: 22606265
  17. RA via RALDH2 has separable functions in the developing spinal cord to (i) maintain high levels of FGF and Notch signaling and (ii) drive stem cell differentiation, thus restricting both the numbers and the pluripotent character of neural stem cells PMID: 22396766
  18. discovery of a phenotype for the FGF1 knockout mouse establishes the PPARgamma-FGF1 axis as critical for maintaining metabolic homeostasis and insulin sensitization PMID: 22522926
  19. The results demonstrate that the FGF1/FGFR1 complex constitutes a signalling module that independently of the receptor tyrosine kinase can convey a signal that initiates a strictly timed and periodic release of endocytosed FGF1 into the cytosol/nucleus. PMID: 21223966
  20. RFX1 may negatively regulate the self-renewal of GBM-SCs through modulating FGF-1B and FGF1 expression levels by binding the 18-bp cis-elements of the F1B promoter. PMID: 20189986
  21. the neuroprotective effects of FGF1 involve inactivation of GSK3beta PMID: 12095987
  22. FGF-1, FGF-2 and FGF receptor-1 levels in the cochlear nucleus following acoustic overstimulation PMID: 12121735
  23. the depletion of FGF1 may be a key regulatory component in initial phase of branching morphogenesis of the lung bud epithelium in vitro PMID: 12128203
  24. Agonist role for FGF1 and FGF2 in specifically insult-induced liver matrix deposition and hepatic fibrogenesis and a potential target for the prevention of hepatic fibrosis. PMID: 14507672
  25. non-classical release is mediated by synaptotagmin 1 PMID: 14559220
  26. High steady-state levels of ONOO(-) may induce cysteine oxidation, tyrosine nitration, and non-nreversible inactivation of FGF-1, a inhibitory feedback mechanism restoring cellular homeostatis during resolution of inflammation and repair. PMID: 14592461
  27. Gab1 is essential for FGF1 stimulation of both PI 3-kinase and the antiapoptotic protein kinase Akt, while FGF1-induced MAPK stimulation is not affected by Gab1 deficiency. PMID: 15199124
  28. Data show that mouse and human fibroblast growth factor 1 (FGF-1) internal ribosome entry sites show similar activity profiles, and have a conserved structural domain at both the nucleotide sequence and RNA structure levels. PMID: 15314170
  29. Phosphorylation of Fgf1 occurs in the nucleus by pkcdelta. It is then exported to the cytosol. PMID: 15574884
  30. Hsp90 is required for translocation of FGF-1 and FGF-2 across the endosomal membrane PMID: 16495214
  31. FGF7- and FGF1-induced mitogenesis and downstream signaling is controlled by distinct heparin octasaccharide motifs PMID: 16728399
  32. FGF1 and p40 synaptotagmin 1 release correlates with membrane destabilizing ability PMID: 16930531
  33. These results identify a novel aspect of the crosstalk between FGF and thrombin signaling pathways which both play important roles in tissue repair and angiogenesis. PMID: 17027650
  34. Shisa is antagonistic to Wnt and Fgf signalings PMID: 17481602
  35. In apoE-deficient mouse astrocytes, FGF-1 stimulated cholesterol biosynthesis without enhancing its release, indicating a signaling pathway independent of apoE biosynthesis upregulation. PMID: 17548887
  36. Formation of the FGF-1-exportin-1-Ran-GTP complex in vitro as well as nuclear export of FGF-1 in vivo was dependent on phosphorylation of FGF-1, and it was abolished by leptomycin B. PMID: 17616529
  37. sphingosine kinase 1 is a component of the copper-dependent FGF1 release pathway. PMID: 17643421
  38. A conserved, specific and stage-dependent requirement for Erk1/2 signalling downstream of FGF-induced neural specification in higher vertebrates. PMID: 17660197
  39. FGF and PDGF have roles in cell proliferation and migration in osteoblastic cells PMID: 17852407
  40. analysis of expression of bicistronic vector driven by the FGF-1 IRES in mouse muscle PMID: 17963525
  41. 6-O-sulfate in HS may regulate the signalings of some of HB-GFs, including FGF-1, FGF-2 and FGF-4, by inducing different interactions between ligands and their receptors PMID: 18281280
  42. and fibroblast growth factor signaling interact to control the proximal-distal pattern of forming airways in the mammalian lung PMID: 18694942
  43. These data demonstrate the existence of novel cross-talk between thrombin, FGF, and Notch signaling pathways, which play important roles in vascular formation and remodeling. PMID: 18784255
  44. An interplay between retinoic acid, Fgf and Shh signalling is likely to be an important mechanism underpinning the tight regulation of caudal embryonic development. PMID: 19168680
  45. A detailed double-label immunohistochemical investigation of the localization patterns of FGF1 and its receptors FGFR1 and FGFR2 in adult and early postnatal mouse retinas, is reported. PMID: 19408015
  46. Results revealed a mechanism of molecular coupling of mRNA transcription and translation, involving a unique process of IRES activation by a FGF1 promoter element. PMID: 19561198
  47. that the main role of heparin in FGF-induced signaling is to protect this naturally unstable protein against heat and/or proteolytic degradation and heparin is not essential for a direct FGF1-FGFR interaction and receptor activation PMID: 19574212

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Subcellular Location Secreted, Cytoplasm, Cytoplasm, cell cortex, Cytoplasm, cytosol, Nucleus
Protein Families Heparin-binding growth factors family
Database Links

KEGG: mmu:14164

STRING: 10090.ENSMUSP00000045710

UniGene: Mm.241282

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