The fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. These domains shape the paratope — the antigen-binding site — at the amino terminal end of the monomer. The two variable domains bind the epitope on their specific antigens.
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ELISA, WB, SDS-PAGE
PBS, pH 7.4
Full length protein
Rat serum IgG digested with papain
Rat IgG Fab fragment
Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.