Recombinant Bacillus subtilis Uncharacterized protein YuaB (yuaB)

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Code CSB-CF300296BRJ
MSDS
Size $878
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized yuaB at 5 μg/ml can bind human HP_0175 with a linear range of 31.25-600.00 ng/ml.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
yuaB
Uniprot No.
Research Area
Others
Alternative Names
bslA; yuaB; BSU31080; Biofilm-surface layer protein A; ORF-1
Species
Bacillus subtilis (strain 168)
Source
in vitro E.coli expression system
Expression Region
1-181aa
Target Protein Sequence
MKRKLLSSLAISALSLGLLVSAPTASFAAESTSTKAHTESTMRTQSTASLFATITGASKTEWSFSDIELTYRPNTLLSLGVMEFTLPSGFTANTKDTLNGNALRTTQILNNGKTVRVPLALDLLGAGEFKLKLNNKTLPAAGTYTFRAENKSLSIGNKFYAEASIDVAKRSTPPTQPCGCN
Mol. Weight
35.3kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Involved in biofilm formation. Self-polymerizes and forms a layer on the surface of biofilms that confers hydrophobicity to the biofilm. The layer is stable and capable of resistance to high mechanical force compression. Required for complex colony architecture. May function synergistically with exopolysaccharides and TasA amyloid fibers to facilitate the assembly of the biofilm matrix.
Gene References into Functions
  1. Taken together, these findings identify YuaB as a protein that plays a novel role during biofilm formation. PMID: 21742882
Subcellular Location
Secreted. Secreted, cell wall.
Database Links
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