Recombinant Bacillus subtilis Uncharacterized protein YuaB(yuaB) (Active)

In Stock
Code CSB-CF300296BRJ
Size US$3197
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized yuaB at 5 μg/ml can bind human HP_0175 with a linear range of 31.25-600.00 ng/ml.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Activity Measured by its binding ability in a functional ELISA. Immobilized yuaB at 5 μg/ml can bind human HP_0175 with a linear range of 31.25-600.00 ng/ml.
Target Names yuaB
Uniprot No. P71014
Research Area Others
Alternative Names bslA; yuaB; BSU31080; Biofilm-surface layer protein A; ORF-1
Species Bacillus subtilis (strain 168)
Source in vitro E.coli expression system
Expression Region 1-181aa
Target Protein Sequence MKRKLLSSLAISALSLGLLVSAPTASFAAESTSTKAHTESTMRTQSTASLFATITGASKTEWSFSDIELTYRPNTLLSLGVMEFTLPSGFTANTKDTLNGNALRTTQILNNGKTVRVPLALDLLGAGEFKLKLNNKTLPAAGTYTFRAENKSLSIGNKFYAEASIDVAKRSTPPTQPCGCN
Mol. Weight 35.3kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-SUMO-tagged
Form Lyophilized powder
Buffer Lyophilized from 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Involved in biofilm formation. Self-polymerizes and forms a layer on the surface of biofilms that confers hydrophobicity to the biofilm. The layer is stable and capable of resistance to high mechanical force compression. Required for complex colony architecture. May function synergistically with exopolysaccharides and TasA amyloid fibers to facilitate the assembly of the biofilm matrix.
Gene References into Functions
  1. Taken together, these findings identify YuaB as a protein that plays a novel role during biofilm formation. PMID: 21742882
Subcellular Location Secreted. Secreted, cell wall.
Database Links

KEGG: bsu:BSU31080

STRING: 224308.Bsubs1_010100016901

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