Name: Recombinant Human Claudin-18.2(CLDN18.2)-VLPs (Active)
Brand: CUSABIO
SKU: CSB-MP005498HU(A5)

Product Details

Description	This recombinant human claudin-18.2 (CLDN 18.2) is prepared in mammalian cells. Its target gene encodes the full human claudin-18.2 (CLDN 18.2), containing a 10xHis-tag at the C-terminus. The CLDN 18.2 protein has been validated to be active through a functional ELISA. Immobilized human CLDN 18.2 protein can bind to the recombinant anti-CLDN 18.2 monoclonal antibody. And the EC₅₀ is 5.225-9.256 ng/ml. In the WB assay, this CLDN 18.2 protein was detected by the mouse anti-6xHis monoclonal antibody. Three bands appeared on the gel electrophoresis, respectively corresponding to monomer, homodimer, homotrimer of the CLDN 18.2. CLDN 18.2, the isoform of CLDN 18, is a tight junction protein exclusively expressed in differentiated epithelial cells of gastric mucosa under normal physiological conditions. Studies have demonstrated that CLDN 18.2 was highly expressed in primary gastric malignancies and post-metastatic types of cancer, including carcinomas of the pancreas, esophagus, ovaries, and lungs. Therefore, CLDN 18.2 has become an ideal target for researchers to develop immunotherapies for solid tumors such as gastric cancer and pancreatic cancer.
Endotoxin	Less than 1.0 EU/ug as determined by LAL method.
Activity	Measured by its binding ability in a functional ELISA. Immobilized human CLDN18.2 (CSB-MP005498HU(A5)) at 5 μg/ml can bind anti-CLDN18.2 recombinant Monoclonal Antibody (CSB-RA005498A1HU), the the EC₅₀ is 5.225-9.256 ng/ml.
Target Names	CLDN18.2
Uniprot No.	P56856
Alternative Names	CLDN18; UNQ778/PRO1572; Claudin-18
Molecular Characterization
Species	Homo sapiens (Human)
Source	Mammalian cell
Expression Region	1-261aa
Target Protein Sequence	MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKIYDGGARTEDEVQSYPSKHDYV
Mol. Weight	29.1 kDa
Protein Length	Full Length of Isoform A2
Tag Info	C-terminal 10xHis-tagged (This tag can be tested only under denaturing conditions)
Form	Liquid Note: We will default ship it in lyophilized form with normal bule ice packs. However, if you request to ship in liquid form, it needs to be shipped with dry ice, please communicate with us in advance and extra fees for dry ice and dry ice box will be charged.
Buffer	PBS, pH 7.4, 6% trehalose
Troubleshooting and FAQs	Protein FAQs
Storage Condition	Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time	Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. The immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Datasheet & COA	Please contact us to get it.

Target Background

Function	Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Gene References into Functions	In this study, we found downregulation of miR-767-3p and upregulation of CLDN18 in lung adenocarcinoma tissue and cell lines. PMID: 29169410 CDH17 and CLDN18 are useful target molecules. Their coupling can aid in the comprehensive detection and localization of gastric cancer metastases in vivo to overcome challenges associated with intratumoral heterogeneity. PMID: 27580354 Bile duct adenocarcinoma cells overexpress claudin-18 via the EGFR/RAS/ERK pathway, contributing to cell proliferation and invasion. PMID: 28624624 The presented data substantiate the hypothesis that claudin-18 is a central barrier-forming component of tight junctions and show that IL-13 downregulates claudin-18. These data also suggest that the loss of claudin-18 is associated with increased sensitization to aeroantigens and airway responsiveness PMID: 27215490 suggest that the reduction of CLDN5, 7, and 18 expression loses the suppressive ability of interaction between PDK1 and Akt and causes sustained phosphorylation of Akt, resulting in the disordered proliferation in lung squamous carcinoma cells PMID: 27884700 Data suggest that claudin-18 suppresses the abnormal proliferation and motility of lung epithelial cells mediated by inhibition of phosphorylation of pyruvate dehydrogenase kinase isoform 1 (PDK1) and proto-oncogene protein c-akt (Akt). PMID: 26919807 Human fetal lungs at 23-24 weeks gestational age, the highest-risk period for developing bronchopulmonary dysplasia, a disease of impaired alveolarization, had significantly lower CLDN18 expression relative to postnatal lungs. PMID: 24787463 Evaluated expression of claudins in gastric cancer and determined their significance for patient outcome. Claudin-3 and claudin-7 were expressed in 25.4% and 29.9% of gastric cancer tissues; 51.5% of gastric cancer tissues had reduced claudin-18. PMID: 24333468 High levels of CLDN18 are associated with non-small-cell lung cancer. PMID: 24710653 Downregulation of miR-1303 can inhibit proliferation, migration and invasion of gastric cancer cells by targeting CLDN18. PMID: 24647998 Claudin-18 positivity is a specific phenotype that is characteristic of intestinal-type Mucinous borderline tumours of the ovary PMID: 23905715 Down-regulation of claudin-18 is associated with the proliferative and invasive potential of gastric cancer. PMID: 24073219 rate of CLDN18.2 positivity is high in pancreatic neoplasms whereby the expression is not limited to the primaries but is also maintained upon metastasis PMID: 23900716 Claudin 10/18 are most commonly expressed in lung adenocarcinomas. Female patients and non-smokers express these claudins more commonly suggesting that they may play a part in the carcinogenesis of tobacco unrelated carcinoma. PMID: 22076167 Claudin 18 (a marker for early carcinogenesis) is commonly expressed in precursor lesions of pancreatic ductal adenocarcinomas. Activation of the protein kinase C pathway might be involved in claudin 18 expression associated with carcinogenesis. PMID: 21832145 Cldn18 is primarily regulated at the transcriptional level via specific protein kinase C signaling pathways and modified by DNA methylation PMID: 21381080 These results suggest that CLDN18 may play an important role in biliary carcinogenesis. PMID: 21607649 High claudin 18 is associated with intraductal papillary mucinous neoplasms of the pancreas. PMID: 21206985 We revealed that claudin-18 expression correlates with poor survival in patients with colorectal cancer and is associated with the gastric phenotype. PMID: 20846265 Loss of claudin expression may enhance the grade of malignancy of gastric cancer in vivo. PMID: 17459057 We conclude that Cldn-18 is the dominant claudin in the TJ of SCE and propose that the change from a Cldn-18-deficient TJ in SqE to a Cldn-18-rich TJ in SCE contributes to the greater acid resistance of BE. PMID: 17932229 Indicate that the PKC/MAPK/AP-1 dependent pathway regulates claudin-18a2 expression in gastric cells. PMID: 18032479 Claudin 18 and annexin A8 are frequently highly overexpressed in infiltrating ductal adenocarcinomas. PMID: 18223320 Claudin 18 staining can aid in diagnosis of gastrointestinal signet ring cell carcinoma. PMID: 18580680 Increased expression of claudin-18 is associated with colitis. PMID: 18831034 CLDN18.2 as a novel, highly attractive pan-cancer target for the antibody therapy of epithelial tumors. PMID: 19047087 Show More Hide All
Subcellular Location	Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
Protein Families	Claudin family
Tissue Specificity	Isoform A1: Expression is restricted to the lung. Isoform A2: Expression is restricted to the stomach mucosa where it is predominantly observed in the epithelial cells of the pit region and the base of the gastric glands including exocrine and endocrine c
Database Links	HGNC: 2039 OMIM: 609210 KEGG: hsa:51208 STRING: 9606.ENSP00000183605 UniGene: Hs.655324

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Code	CSB-MP005498HU(A5)
Size	$4940
Image	Western Blot CSB-MP005498HU(A5) is detected by Mouse anti-6*His monoclonal antibody.The three bands respectively correspond to monomer, Homodimer, Homotrimer Activity Measured by its binding ability in a functional ELISA. Immobilized human CLDN18.2 (CSB-MP005498HU(A5)) at 5 μg/ml can bind anti-CLDN18.2 recombinant Monoclonal Antibody (CSB-RA005498A1HU), the the EC₅₀ is 5.225-9.256 ng/ml. Biological Activity Assay The presence of VLP-like structures was confirmed by TEM Western Blot CSB-MP005498HU(A5) is detected by Anti-Claudin 18 Antibody.The two bands respectively correspond to monomer, Homodimer
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Recombinant Human Claudin-18.2(CLDN18.2)-VLPs (Active)

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