Claudin-18.2 Recombinant Monoclonal Antibody

Code CSB-RA005498A1HU
Size US$210
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  • Untransfected KATO3 cells surface (green line), transfected Human CLDN18.2 KATO3 stable cells surface (red line) and transfected Human CLDN18.1 NIH-3T3 stable cells surface (blue line) were stained with anti-CLDN18.2 antibody (2µg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
  • The Binding Activity of Human CLDN18.2 with Anti-CLDN18.2 recombinant antibody.
    Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 (CSB-MP005498HU(A5)) at 5 μg/mL can bind Anti-CLDN18.2 recombinant antibody, the EC50 is 6.554-27.87 ng/mL.
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Product Details

Uniprot No.
Target Names
Alternative Names
Claudin-18, CLDN18
Species Reactivity
Recombinant Human Claudin-18.2 protein
Immunogen Species
Homo sapiens (Human)
Clone No.
Purification Method
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Tested Applications
Recommended Dilution
Application Recommended Dilution
FC 1:50-1:200
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

The creation of the Claudin-18.2 recombinant monoclonal antibody involves a meticulous process to ensure its high quality and specificity. Initially, B cells are isolated from the spleen of an immunized animal, with the recombinant human Claudin-18.2 protein serving as the immunogen. The RNA extracted from the B cells is converted into cDNA through reverse transcription. The Claudin-18.2 antibody genes are then amplified using specific primers targeting the antibody constant regions and inserted into an expression vector. This vector is subsequently transfected into host cells, facilitating the production of the Claudin-18.2 recombinant monoclonal antibody. After a period of cell culture, the antibody is collected from the cell culture supernatant and purified using affinity chromatography, yielding a highly purified form suitable for diverse applications. Stringent characterization assays, including ELISA and FC analysis, are performed to validate the antibody's specificity and functionality in detecting human Claudin-18.2 protein. The meticulous production process guarantees the development of a reliable and effective Claudin-18.2 recombinant monoclonal antibody, which plays a vital role in various Claudin-18.2-related research.

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 2 reviews

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Applications : FC

Sample type: cell

Review: Normal assay process. The prodcuts performes well in the whole assay.

By Anonymous

Applications : Immunohistochemistry (IHC), Flow Cytometry (FC), ELISA

Sample type: Human gastric mucosa cells

Sample dilution: 1:100

Review: I used antibodies to CSB-RA005498A1HU, ELISA and WB for human cells, and the band of the band was as expected. The EC50 by ELISA was 2.623-14.06 ng / mL. Good antibody specificity and high sensitivity.

By Mr.peng

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