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| Code | CSB-RA005498A1HU |
| Size | US$210 |
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| Application | Recommended Dilution |
|---|---|
| FC | 1:50-1:200 |
The creation of the Claudin-18.2 recombinant monoclonal antibody involves a meticulous process to ensure its high quality and specificity. Initially, B cells are isolated from the spleen of an immunized animal, with the recombinant human Claudin-18.2 protein serving as the immunogen. The RNA extracted from the B cells is converted into cDNA through reverse transcription. The Claudin-18.2 antibody genes are then amplified using specific primers targeting the antibody constant regions and inserted into an expression vector. This vector is subsequently transfected into host cells, facilitating the production of the Claudin-18.2 recombinant monoclonal antibody. After a period of cell culture, the antibody is collected from the cell culture supernatant and purified using affinity chromatography, yielding a highly purified form suitable for diverse applications. Stringent characterization assays, including ELISA and FC analysis, are performed to validate the antibody's specificity and functionality in detecting human Claudin-18.2 protein. The meticulous production process guarantees the development of a reliable and effective Claudin-18.2 recombinant monoclonal antibody, which plays a vital role in various Claudin-18.2-related research.
Applications : FC
Sample type: cell
Review: Normal assay process. The prodcuts performes well in the whole assay.
By Anonymous
Applications : Immunohistochemistry (IHC), Flow Cytometry (FC), ELISA
Sample type: Human gastric mucosa cells
Sample dilution: 1:100
Review: I used antibodies to CSB-RA005498A1HU, ELISA and WB for human cells, and the band of the band was as expected. The EC50 by ELISA was 2.623-14.06 ng / mL. Good antibody specificity and high sensitivity.
By Mr.peng
