Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its ability to hydrolyze p-nitrophenylacetate. The specific activity is >40000 pmol/min/µg.

Target Names

CES2

Uniprot No.

O00748

Alternative Names

Cocaine esterase; EC:3.1.1.84; Carboxylesterase 2 (CE-2; hCE-2) (EC:3.1.1.11); Methylumbelliferyl-acetate deacetylase 2 (EC:3.1.1.56); CES2;ICE

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

27-559aa

Target Protein Sequence

QDSASPIRTTHTGQVLGSLVHVKGANAGVQTFLGIPFAKPPLGPLRFAPPEPPESWSGVRDGTTHPAMCLQDLTAVESEFLSQFNMTFPSDSMSEDCLYLSIYTPAHSHEGSNLPVMVWIHGGALVFGMASLYDGSMLAALENVVVVIIQYRLGVLGFFSTGDKHATGNWGYLDQVAALRWVQQNIAHFGGNPDRVTIFGESAGGTSVSSLVVSPISQGLFHGAIMESGVALLPGLIASSADVISTVVANLSACDQVDSEALVGCLRGKSKEEILAINKPFKMIPGVVDGVFLPRHPQELLASADFQPVPSIVGVNNNEFGWLIPKVMRIYDTQKEMDREASQAALQKMLTLLMLPPTFGDLLREEYIGDNGDPQTLQAQFQEMMADSMFVIPALQVAHFQCSRAPVYFYEFQHQPSWLKNIRPPHMKADHGDELPFVFRSFFGGNYIKFTEEEEQLSRKMMKYWANFARNGNPNGEGLPHWPLFDQEEQYLQLNLQPAVGRALKAHRLQFWKKALPQKIQELEEPEERHTEL

Mol. Weight

60.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Carboxylesterase 2 hydrolyzes a broad spectrum of ester-containing xenobiotics and endogenous lipids, making it a critical target for understanding drug metabolism, prodrug activation, and detoxification pathways in human tissues. This full-length mature protein (aa 27–559) demonstrates robust catalytic function with a specific activity exceeding 40,000 pmol/min/µg against p-nitrophenylacetate, providing a quantitative benchmark that supports kinetic parameter analysis including Km, Vmax, and kcat/Km determination. The C-terminal His tag preserves native enzyme architecture and active site accessibility, enabling inhibitor screening with accurate IC50 measurements and substrate specificity profiling across diverse ester substrates. Purity greater than 95% by SDS-PAGE and endotoxin levels below 1.0 EU/µg satisfy the stringent criteria expected for enzyme kinetics studies, drug candidate evaluation, and use as a positive control in high-throughput enzyme-linked assays.

Target Background

Function

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine. Hydrolyzes aspirin, substrates with large alcohol group and small acyl group and endogenous lipids such as triacylglycerol. Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins.

Gene References into Functions

SNPs in CDA and CES2 were associated with benefit from the addition of capecitabine to chemotherapy in metastatic breast cancer patients. PMID: 28827188
Results revealed that most of the colorectal cancer specimens showed a considerable reduction in CES2 expression compared with adjacent normal tissue independently of p53. PMID: 29651325
Anordrin is predominantly catalyzed by CES1 and CES2 to generate the main active metabolite, anordiol. PMID: 28532270
These data suggest that infants younger than 3 weeks of age would exhibit significantly lower CES1- and CES2-dependent metabolic clearance compared with older individuals. PMID: 26825642
Carboxylesterase 2 possesses triglyceride and diacylglycerol lipase activities and displayed an inverse correlation with HOMA-IR and hepatic diacylglycerol concentrations in humans. PMID: 28099843
has triglyceride hydrolase activity; as a result, gain of hepatic CES2 function increases fatty acid oxidation and inhibits lipogenesis, whereas loss of hepatic CES2 stimulates lipogenesis by inducing endoplasmic reticulum stress PMID: 26806650
Plasma cells are strong producers of CES-2 in intestinal inflammation and cancer and may be involved in metabolic activation of ester-containing prodrugs. PMID: 27149931
Data show that carboxylesterase 2 (CES2A1) is the primary mediator of carboxylesterase (CES) substrates in the enterocytes. PMID: 28285137
Findings suggest that CES2 expression and activity, by mediating the intratumoral activation of irinotecan, is a contributor to FOLFIRINOX sensitivity in pancreatic cancer. PMID: 26025324
In the liver and duodenum, CES2 mRNA expression increased and exhibited a postnatal surge. PMID: 25724353
After oral administration of dabigatran etexilate to humans, DABE is hydrolyzed by intestinal CES2 to the intermediate M2 metabolite followed by hydrolysis of M2 to DAB in the liver by CES1. PMID: 24212379
expression of CES2, UGTA1A1, and GUSB varies in colorectal pathology tissues and that the expression of CES2 is somewhat related to tumor staging. PMID: 24195516
Variations in CES2 in the promoter region, may alter rifampicin metabolism by affecting expression of the gene PMID: 23471941
CES2 RNA expression was observed in neuroblastoma cells, and its expression in neuroblastoma cell lines was positively correlated with sensitivity to CPT-11 and apoptosis after CPT-11 exposure in vitro PMID: 23480903
tested the hypothesis that an individual's CES phenotype can be characterized by reporter substrates/probes that interrogate native CES1 and CES2 activities in liver and immunoblotting methods PMID: 22525521
Report CES2 mediated hydrolysis of heroin, cocaine and CPT-11. PMID: 20649590
A comparison of the substrate specificity of CES1 versus CES2 reveals broad but distinct substrate preferences. PMID: 19508181
The multiple promoters in the CES2 gene were characterized. PMID: 12835618
Single-nucleotide polymorphism in carboxylesterase 2 is associated with reduced mRNA expression in colorectal tumors PMID: 15475243
analysis of single nucleotide polymorphisms and haplotype structure of the human carboxylesterase 2 gene PMID: 15475733
human carboxylesterase 2 gene presents several polymorphisms, none of which seems to be involved in significant variations in protein activity PMID: 15592324
TFF-1 is lost late in the progression from Barrett's Esophagus to adenocarcinoma, while CES-2 is upregulated PMID: 15967118
in diffuse large B-cell lymphoma, molecular alterations in ice, bcl-2, c-myc and p53 are present in hematopoietic cells from bone marrow as well as in primitive hematopoietic progenitors PMID: 16690525
p53 directly regulates CES-2 expression in a colonic cancer cell line. PMID: 16963839
830C>G single nucleotide polymorphism of CES2 is unlikely to have significant functional consequences on CES2 expression, activity or function PMID: 17483951
IL-6 alters the cellular responsiveness to clopidogrel, irinotecan, and oseltamivir by suppressing the expression of CES2. PMID: 17537833
In a Japanese population, CES2 haplotypes and a defective allele were associated with a decrease in enzyme levels or activity. Pharmacokinetics of irinotecan was evaluated in cancer patients. PMID: 17640957
comparative analysis of CES1 and CES2 in liver and small intestine of humans, monkeys, dogs, rabbits and rats PMID: 17764701
Carboxylesterase 2 is downregulated in colorectal cancer following progression of the disease PMID: 18259949
Carboxylesterase 1A2 has been isolated and determined to be identical to carboxylesterase 1A1 except for exon 1 and the 5' regulatory element. PMID: 18305377
an association between a polymorphism in the CES2 gene and the efficacy of capecitabine has been described PMID: 18473752
pharmacogenomic characterization of human carboxylesterase 1A1, 1A2, and 1A3 genes PMID: 18794728
Carboxylesterases play critical roles in drug metabolism and insecticide detoxication; findings show large variability among different age groups or even within the same age group. PMID: 18983829
serum carboxylesterase-2 level might be a potential marker in the diagnosis of the early stage ovarian cancer. PMID: 19856659

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Subcellular Location

Endoplasmic reticulum lumen.

Protein Families

Type-B carboxylesterase/lipase family

Tissue Specificity

Preferentially expressed in intestine with moderate expression in liver. Within the intestine, highest expression is found in small intestine with lower expression in colon and rectum.

Database Links

HGNC: 1864

OMIM: 605278

KEGG: hsa:8824

STRING: 9606.ENSP00000317842

UniGene: Hs.282975

Code	CSB-MP005259HU
Abbreviation	Recombinant Human CES2 protein (Active)
MSDS	MSDS Datasheet
Size	$138
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its ability to hydrolyze p-nitrophenylacetate. The specific activity is >40000 pmol/min/µg. Biological Activity Assay
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Recombinant Human Cocaine esterase (CES2) (Active)

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