Recombinant Macaca fascicularis Inositol 1,4,5-trisphosphate receptor-interacting protein-like 1 (ITPRIPL1), partial (Active)

ITPRIPL1 interacts with inositol 1,4,5-trisphosphate receptors and participates in calcium signaling pathways, making it a valuable tool for dissecting intracellular calcium dynamics and receptor-mediated signaling events. This partial construct spanning residues 25–103 demonstrates robust antibody-binding activity in functional ELISA, achieving an EC50 of 3.759–4.428 ng/mL when immobilized at 2 μg/mL and tested against an anti-ITPRIPL1 recombinant antibody, confirming proper folding and epitope accessibility. Mammalian cell expression preserves native post-translational modifications critical for authentic protein-protein interactions, supporting its use in binding assays, antibody validation studies, and as a positive control in immunoassays targeting ITPRIPL1. The protein exceeds 95% purity by SDS-PAGE and contains less than 1.0 EU/μg endotoxin, meeting the quality thresholds commonly required for cell-based functional assays where low endotoxin levels are essential to avoid confounding inflammatory responses.