AARS Antibody

Code CSB-PA001023LA01HU
Size US$299
Image
  • IHC image of CSB-PA001023LA01HU diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA001023LA01HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA001023LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) AARS Polyclonal antibody
Uniprot No. P49588
Target Names AARS
Alternative Names AARS antibody; AI316495 antibody; Alanine tRNA ligase 1, cytoplasmic antibody; Alanine tRNA ligase antibody; Alanine tRNA ligase cytoplasmic antibody; Alanine--tRNA ligase antibody; Alanyl tRNA synthetase antibody; Alanyl tRNA synthetase cytoplasmic antibody; Alanyl-tRNA synthetase antibody; AlaRS antibody; C76919 antibody; CMT2N antibody; cytoplasmic antibody; EC 6.1.1.7 antibody; MGC37368 antibody; Renal carcinoma antigen NY REN 42 antibody; Renal carcinoma antigen NY-REN-42 antibody; SYAC_HUMAN antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Alanine--tRNA ligase, cytoplasmic protein (164-321AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:20-1:200
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction
Gene References into Functions
  1. A large sequence divergence of the C-terminal domain (C-Ala) reshaped C-Ala in a way that changed the global architecture of alanyl-tRNA synthetase (AlaRS). This reshaping removed the role of C-Ala in prokaryotes for docking tRNA and instead repurposed it to form a dimer interface presenting a DNA-binding groove. PMID: 27911835
  2. Number of missense mutations in AARS expand the clinical spectrum and provide pheno-genotypic correlations in AARS-related neuropathies. PMID: 26032230
  3. A novel mutation in alanyl-tRNA synthetase causes a mild myeloneuropathy, a novel phenotype for patients with mutations in one of the tRNA synthetase genes. PMID: 25904691
  4. Loss-of-function alanyl-tRNA synthetase mutations cause an autosomal-recessive early-onset epileptic encephalopathy with persistent myelination defect. PMID: 25817015
  5. the pathological consequences of diminished tRNA synthetase editing activity, and thus translational infidelity, are dependent on the cell type and the extent of editing disruption PMID: 25422440
  6. in a family with distal hereditary motor neuropathy (dHMN), all 4 affected family members had a heterozygous missense mutation c.2677G>A (p.D893N) of (AARS), not found in the 4 unaffected members and control subjects; conclude AARS mutation caused dHMN in a Chinese family; AARS mutations result in not only a CMT phenotype but also a dHMN phenotype PMID: 22573628
  7. Methylation-mediated deamination of a CpG dinucleotide gives rise to the recurrent p.Arg329His alanyl-tRNA synthetase mutation in patients with Charcot-Marie-Tooth disease type 2N (CMT2N). PMID: 22009580
  8. We show here that mutations in AARS2 cause perinatal or infantile cardiomyopathy with near-total combined mitochondrial respiratory chain deficiency in the heart. PMID: 21549344
  9. cytoplasmic Alanyl-tRNA synthetase may have a role in dominant axonal Charcot-Marie-Tooth disease, as shown by its mutation in a major determinant for binding and aminoacylation PMID: 20045102

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Involvement in disease Charcot-Marie-Tooth disease 2N (CMT2N); Epileptic encephalopathy, early infantile, 29 (EIEE29)
Subcellular Location Cytoplasm
Protein Families Class-II aminoacyl-tRNA synthetase family
Database Links

HGNC: 20

OMIM: 601065

KEGG: hsa:16

STRING: 9606.ENSP00000261772

UniGene: Hs.315137

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