ABCC5 Antibody

Datasheet
Code CSB-PA001064LA01HU
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Size US$299
Uniprot No. O15440
Image
  • IHC image of CSB-PA001064LA01HU diluted at 1:400 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA001064LA01HU diluted at 1:400 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of PC-3 cells with CSB-PA001064LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunogen Recombinant Human Multidrug resistance-associated protein 5 protein (455-566AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, IF; Recommended dilution: IHC:1:200-1:500, IF:1:50-1:200
Relevance Acts as a multispecific organic anion pump which can transport nucleotide analogs.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Multidrug resistance-associated protein 5 (ATP-binding cassette sub-family C member 5) (Multi-specific organic anion transporter C) (MOAT-C) (SMRP) (pABC11), ABCC5, MRP5
Immunogen Species Human
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Signal Transduction
Target Names ABCC5
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Acts as a multispecific organic anion pump which can transport nucleotide analogs.
Subcellular Location Membrane, Multi-pass membrane protein
Protein Families ABC transporter superfamily, ABCC family, Conjugate transporter (TC 3.A.1.208) subfamily
Tissue Specificity All isoforms are equally expressed in retina.
Database Links

HGNC: 56

OMIM: 605251

KEGG: hsa:10057

STRING: 9606.ENSP00000333926

UniGene: Hs.368563

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