AKTIP Antibody

Datasheet

Code	CSB-PA862043LA01HU
Size	US$166
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Image	IHC image of CSB-PA862043LA01HU diluted at 1:700 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of SH-SY5Y cells with CSB-PA862043LA01HU at 1:140, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) AKTIP Polyclonal antibody

Uniprot No.

Q9H8T0

Target Names

AKTIP

Alternative Names

AKT interacting protein antibody; AKT-interacting protein antibody; AKTIP antibody; AKTIP_HUMAN antibody; FT 1 antibody; FT1 antibody; Fused toes homolog antibody; Fused toes protein homolog antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human AKT-interacting protein (1-292AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The AKTIP Antibody (Product code: CSB-PA862043LA01HU) is Non-conjugated. For AKTIP Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA862043LB01HU	AKTIP Antibody, HRP conjugated	ELISA
FITC	CSB-PA862043LC01HU	AKTIP Antibody, FITC conjugated
Biotin	CSB-PA862043LD01HU	AKTIP Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). Regulates apoptosis by enhancing phosphorylation and activation of AKT1. Increases release of TNFSF6 via the AKT1/GSK3B/NFATC1 signaling cascade. FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell.

Gene References into Functions

Results show that AKTIP co-purifies with A- and B-type lamins and partially co-localizes with lamins in interphase nuclei. In addition, AKTIP depletion lowers lamin A expression and induces senescence hallmarks including telomere homeostasis in human primary fibroblasts. PMID: 27512140
results suggest that AKT1, FTO, and AKTIP polymorphisms were not associated with obesity/overweight in Brazilians children. PMID: 27342216
FTS is involved in EGFR-mediated repair of DNA damage induced by cisplatin in ME180 cells PMID: 27535835
AKTIP interacts with telomeric DNA and is required for telomere maintenance. PMID: 26110528
High fused toes homolog expression is associated with radioresistance in cervical cancer. PMID: 25151576
FTS silencing reduced EMT and cell migration by EGF treatment. These results demonstrate a novel function for FTS in EGF-mediated EMT process. PMID: 24971934
*AKTIP single nucleotide polymorphisms are not associated with late-onset depression patients compared to healthy elderly controls. PMID: 24022875
Targeted inhibition of FTS led to the shutdown of key elemental characteristics of cervical cancer and could lead to an effective therapeutic strategy. PMID: 21424602
These data unraveled the involvement of new oncoprotein FTS in cervical cancer which plays a central role in carcinogenesis. PMID: 20945372
Demographic and clinical characteristics and AKT1 single markers and haplotypes, but not AKTIP polymorphisms or interactions between AKT1 and AKTIP, are associated with increased risk for suicidal behavior in bipolar patients. PMID: 20132317
Ft1 protein interacts directly with PKB, enhancing the phosphorylation of both of its regulatory sites by promoting its interaction with the upstream kinase PDK1 PMID: 14749367
These data suggest that the FTS/Hook/FHIP complex functions to promote vesicle trafficking and/or fusion via the homotypic vacuolar protein sorting complex. PMID: 18799622

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Subcellular Location

Cytoplasm. Cell membrane; Peripheral membrane protein.

Protein Families

Ubiquitin-conjugating enzyme family, FTS subfamily

Database Links

HGNC: 16710

OMIM: 608483

KEGG: hsa:64400

STRING: 9606.ENSP00000378152

UniGene: Hs.380897

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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