APOOL Antibody

Code CSB-PA001949LA01HU
Size US$166
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  • Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA001949LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human colon tissue using CSB-PA001949LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA001949LA01HU at dilution of 1:100

  • Immunofluorescence staining of U251 cells with CSB-PA001949LA01HU at 1:155,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) APOOL Polyclonal antibody
Uniprot No.
Target Names
APOOL
Alternative Names
AAIR8193 antibody; Apolipoprotein O-like antibody; APOOL antibody; APOOL_HUMAN antibody; CXorf33 antibody; FAM121A antibody; Family with sequence similarity 121A antibody; MGC129748 antibody; Protein FAM121A antibody; UNQ8193 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human MICOS complex subunit MIC27 protein (163-268AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The APOOL Antibody (Product code: CSB-PA001949LA01HU) is Non-conjugated. For APOOL Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA001949LB01HU APOOL Antibody, HRP conjugated ELISA
FITC CSB-PA001949LC01HU APOOL Antibody, FITC conjugated
Biotin CSB-PA001949LD01HU APOOL Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Specifically binds to cardiolipin (in vitro) but not to the precursor lipid phosphatidylglycerol. Plays a crucial role in crista junction formation and mitochondrial function,.
Gene References into Functions
  1. APOOL is a cardiolipin-binding component of the Mitofilin/MINOS protein complex. PMID: 23704930
Subcellular Location
Mitochondrion inner membrane; Multi-pass membrane protein. Mitochondrion.
Protein Families
Apolipoprotein O/MICOS complex subunit Mic27 family
Database Links

HGNC: 24009

OMIM: 300955

KEGG: hsa:139322

STRING: 9606.ENSP00000362268

UniGene: Hs.512181

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