APX1 Antibody

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Datasheet

Code	CSB-PA13479A0Rb
Size	US$166
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Image	Western Blot Positive WB detected in: Arabidopsis thaliana (40μg, 20μg, 10μg) All lanes: APX1 antibody at 4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 28 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana APX1 Polyclonal antibody

Uniprot No.

Q05431

Target Names

APX1

Alternative Names

APX1 antibody; At1g07890 antibody; F24B9.2L-ascorbate peroxidase 1 antibody; cytosolic antibody; AP antibody; AtAPx01 antibody; EC 1.11.1.11 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana L-ascorbate peroxidase 1, cytosolic protein (3-247AA)

Immunogen Species

Arabidopsis thaliana

Conjugate

Non-conjugated

The APX1 Antibody (Product code: CSB-PA13479A0Rb) is Non-conjugated. For APX1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA13479B0Rb	APX1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA13479C0Rb	APX1 Antibody, FITC conjugated
Biotin	CSB-PA13479D0Rb	APX1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Plays a key role in hydrogen peroxide removal. Constitutes a central component of the reactive oxygen gene network.

Gene References into Functions

Loss-of-function of APX1 results in tolerance to Se stress. PMID: 27149098
our results suggest that APX1 gene knockout results in enhanced Pb tolerance mainly through activating the expression of the ATP-bind cassette (ABC)-type transporters and at least partially through GSH -dependent PC synthesis pathway by coordinated control of gene expression. PMID: 28167043
S-nitrosylation of APX1 at cysteine (Cys)-32 enhances its enzymatic activity of scavenging hydrogen peroxide, leading to the increased resistance to oxidative stress. PMID: 25667317
Our study points to a key role for the cytosol in protecting the chloroplast during light stress and provides evidence for cross-compartment protection of thylakoid and stromal/mitochondrial APXs by cytosolic APX1. PMID: 15608336
Lack of a cytosolic hydrogen peroxide removal enzyme triggers a signal which results in stunted growth and enhanced sensitivity to oxidative stress. PMID: 17556505
Data suggest that ascorbate peroxidase is particularly important for photoprotection during the early greening process in Arabidopsis. PMID: 18318659
Ascorbate peroxidase activity was higher in ascorbate-deficient mutants. PMID: 18849295
cytosolic APX1 plays a key role in the acclimation of plants to a combination of drought and heat stress PMID: 18852264

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Subcellular Location

Cytoplasm.

Protein Families

Peroxidase family, Ascorbate peroxidase subfamily

Tissue Specificity

Predominantly expressed in flowers.

Database Links

KEGG: ath:AT1G07890

STRING: 3702.AT1G07890.1

UniGene: At.47584

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Service

Phage Display Service

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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