APX1 Antibody

Code CSB-PA13479A0Rb
Size US$166
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  • Western Blot
    Positive WB detected in: Arabidopsis thaliana (40μg, 20μg, 10μg)
    All lanes: APX1 antibody at 4µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 28 kDa
    Observed band size: 28 kDa
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana APX1 Polyclonal antibody
Uniprot No.
Target Names
APX1
Alternative Names
APX1 antibody; At1g07890 antibody; F24B9.2L-ascorbate peroxidase 1 antibody; cytosolic antibody; AP antibody; AtAPx01 antibody; EC 1.11.1.11 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana L-ascorbate peroxidase 1, cytosolic protein (3-247AA)
Immunogen Species
Arabidopsis thaliana
Conjugate
Non-conjugated

The APX1 Antibody (Product code: CSB-PA13479A0Rb) is Non-conjugated. For APX1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA13479B0Rb APX1 Antibody, HRP conjugated ELISA
FITC CSB-PA13479C0Rb APX1 Antibody, FITC conjugated
Biotin CSB-PA13479D0Rb APX1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Plays a key role in hydrogen peroxide removal. Constitutes a central component of the reactive oxygen gene network.
Gene References into Functions
  1. Loss-of-function of APX1 results in tolerance to Se stress. PMID: 27149098
  2. our results suggest that APX1 gene knockout results in enhanced Pb tolerance mainly through activating the expression of the ATP-bind cassette (ABC)-type transporters and at least partially through GSH -dependent PC synthesis pathway by coordinated control of gene expression. PMID: 28167043
  3. S-nitrosylation of APX1 at cysteine (Cys)-32 enhances its enzymatic activity of scavenging hydrogen peroxide, leading to the increased resistance to oxidative stress. PMID: 25667317
  4. Our study points to a key role for the cytosol in protecting the chloroplast during light stress and provides evidence for cross-compartment protection of thylakoid and stromal/mitochondrial APXs by cytosolic APX1. PMID: 15608336
  5. Lack of a cytosolic hydrogen peroxide removal enzyme triggers a signal which results in stunted growth and enhanced sensitivity to oxidative stress. PMID: 17556505
  6. Data suggest that ascorbate peroxidase is particularly important for photoprotection during the early greening process in Arabidopsis. PMID: 18318659
  7. Ascorbate peroxidase activity was higher in ascorbate-deficient mutants. PMID: 18849295
  8. cytosolic APX1 plays a key role in the acclimation of plants to a combination of drought and heat stress PMID: 18852264

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Subcellular Location
Cytoplasm.
Protein Families
Peroxidase family, Ascorbate peroxidase subfamily
Tissue Specificity
Predominantly expressed in flowers.
Database Links

KEGG: ath:AT1G07890

STRING: 3702.AT1G07890.1

UniGene: At.47584

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