ATP6V1A Antibody

Datasheet

Code	CSB-PA002396LA01HU
Size	US$166
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Image	IHC image of CSB-PA002396LA01HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA002396LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ATP6V1A Polyclonal antibody

Uniprot No.

P38606

Target Names

ATP6V1A

Alternative Names

70kDa subunit antibody; ATP6A1 antibody; ATP6V1A antibody; ATP6V1A1 antibody; ATPase H+ transporting lysosomal subunit A1 antibody; ATPase H+ transporting; lysosomal 70kDa V1 subunit A antibody; H(+) transporting two sector ATPase subunit A antibody; H+ transporting ATPase chain A vacuolar (VA68 type) antibody; HO68 antibody; OTTHUMP00000214746 antibody; V ATPase 69 kDa subunit 1 antibody; V ATPase A subunit 1 antibody; V ATPase subunit A 1 antibody; V-ATPase 69 kDa subunit antibody; V-ATPase subunit A antibody; V-type proton ATPase catalytic subunit A antibody; VA68 antibody; Vacuolar ATP synthase catalytic subunit A ubiquitous isoform antibody; Vacuolar ATPase isoform VA68 antibody; Vacuolar proton pump alpha subunit 1 antibody; Vacuolar proton pump subunit alpha antibody; VATA_HUMAN antibody; Vma1 antibody; VPP2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human V-type proton ATPase catalytic subunit A protein (159-405AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ATP6V1A Antibody (Product code: CSB-PA002396LA01HU) is Non-conjugated. For ATP6V1A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA002396LB01HU	ATP6V1A Antibody, HRP conjugated	ELISA
FITC	CSB-PA002396LC01HU	ATP6V1A Antibody, FITC conjugated
Biotin	CSB-PA002396LD01HU	ATP6V1A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Catalytic subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase), a multisubunit enzyme composed of a peripheral complex (V1) that hydrolyzes ATP and a membrane integral complex (V0) that translocates protons. V-ATPase is responsible for acidifying and maintaining the pH of intracellular compartments and in some cell types, is targeted to the plasma membrane, where it is responsible for acidifying the extracellular environment. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.; (Microbial infection) Plays an important role in virion uncoating during Rabies virus replication after membrane fusion. Specifically, participates in the dissociation of incoming viral matrix M proteins uncoating through direct interaction.

Gene References into Functions

We report biallelic mutations in ATP6V1E1 and ATP6V1A, respectively encoding the E1 and A subunits of the V1 domain of V-ATPase, as a cause of distinct metabolic and multisystemic cutis laxa entities. PMID: 28065471
V1A subunit of V-ATPase has a role in the progression and prognosis of gastric cancer. PMID: 25652905
Data show that the cAMP/PKA/CREB signaling pathway initiates acidosis-induced V-ATPase trafficking in salivary ducts via regulation of Rab11b expression. PMID: 22561749
These data introduce Rab11b as a crucial regulator and Rip11 as mediator of acidosis-induced V-ATPase traffic in duct cells of submandibular gland. PMID: 20717956
there is an important role for physical association between aldolase and the A, B and E subunits of V-ATPase in the regulation of the proton pump PMID: 17576770

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Involvement in disease

Cutis laxa, autosomal recessive, 2D (ARCL2D)

Subcellular Location

Cytoplasm. Cytoplasmic vesicle, secretory vesicle.

Protein Families

ATPase alpha/beta chains family

Tissue Specificity

High expression in the skin.

Database Links

HGNC: 851

OMIM: 607027

KEGG: hsa:523

STRING: 9606.ENSP00000273398

UniGene: Hs.477155

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Related pathways

mTOR signaling pathway Oxidative Phosphorylation Synaptic vesicle cycle

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