ATP6V1C1 Antibody

Datasheet

Code	CSB-PA002399LA01HU
Size	US$166
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Image	IHC image of CSB-PA002399LA01HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA002399LA01HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of MCF-7 cells with CSB-PA002399LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ATP6V1C1 Polyclonal antibody

Uniprot No.

P21283

Target Names

ATP6V1C1

Alternative Names

ATP6C antibody; ATP6D antibody; ATP6V1C1 antibody; ATPase H+ transporting lysosomal (vacuolar proton pump) 42kD antibody; ATPase H+ transporting lysosomal 42kD V1 subunit C isoform 1 antibody; ATPase H+ transporting lysosomal 42kDa V1 subunit C isoform 1 antibody; ATPase H+ transporting lysosomal 42kDa V1 subunit C1 antibody; ATPase H+ transporting lysosomal V1 subunit C1 antibody; FLJ20057 antibody; H(+) transporting two sector ATPase subunit C antibody; H+ ATPase C subunit antibody; H+ transporting ATPase chain C vacuolar antibody; Subunit C of vacuolar proton ATPase V1 domain antibody; V ATPase C subunit antibody; V ATPase subunit C 1 antibody; V-ATPase subunit C 1 antibody; V-type proton ATPase subunit C 1 antibody; Vacuolar ATP synthase subunit C antibody; Vacuolar proton pump 42 kD subunit antibody; Vacuolar proton pump C subunit antibody; Vacuolar proton pump subunit C 1 antibody; Vacuolar protonATPase subunit C VI domain antibody; VATC antibody; VATC1_HUMAN antibody; VATPase C subunit antibody; VATPase subunit C 1 antibody; VMA5 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human V-type proton ATPase subunit C 1 protein (129-382AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ATP6V1C1 Antibody (Product code: CSB-PA002399LA01HU) is Non-conjugated. For ATP6V1C1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA002399LB01HU	ATP6V1C1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA002399LC01HU	ATP6V1C1 Antibody, FITC conjugated
Biotin	CSB-PA002399LD01HU	ATP6V1C1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit C is necessary for the assembly of the catalytic sector of the enzyme and is likely to have a specific function in its catalytic activity. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Gene References into Functions

knockdown of C1 reduces breast cancer growth, metastasis, and osteolytic lesion formation. PMID: 28504970
We assessed ATPaseC1 expression in a sample of oral squamous cell carcinoma using tissue microarrays to analyze the relation between ATPaseC1 expression and clinical, histopathological and prognostic parameters. PMID: 25901422
results of our study suggest that high expression of Atp6v1c1 affects the actin structure of cancer cells such that it facilitates breast cancer metastasis PMID: 24454753
Immunohistochemical localization of C1 subunit of V-ATPase (ATPase C1) in oral squamous cell cancer and normal oral mucosa. PMID: 21526910
proximal promoter region contains cis-acting elements required for expression in cancer cells PMID: 12890556

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Protein Families

V-ATPase C subunit family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 856

OMIM: 603097

KEGG: hsa:528

STRING: 9606.ENSP00000379203

UniGene: Hs.86905

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

mTOR signaling pathway Oxidative Phosphorylation Synaptic vesicle cycle

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