BAZ2B Antibody

Datasheet
Code CSB-PA883427LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q9UIF8
Image
  • IHC image of CSB-PA883427LA01HU diluted at 1:800 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA883427LA01HU diluted at 1:800 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of Hela cells with CSB-PA883427LA01HU at 1:266, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Bromodomain adjacent to zinc finger domain protein 2B protein (139-240AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, IF; Recommended dilution: IHC:1:500-1:1000, IF:1:200-1:500
Relevance May play a role in transcriptional regulation interacting with ISWI.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Bromodomain adjacent to zinc finger domain protein 2B (hWALp4), BAZ2B, KIAA1476
Immunogen Species Human
Research Area Epigenetics and Nuclear Signaling
Target Names BAZ2B
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function May play a role in transcriptional regulation interacting with ISWI.
Subcellular Location Nucleus
Protein Families WAL family
Tissue Specificity Expressed at varying levels in several tissues, whereas a smaller transcript was expressed specifically in testis.
Database Links

HGNC: 963

OMIM: 605683

KEGG: hsa:29994

STRING: 9606.ENSP00000376534

UniGene: Hs.470369

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