BRF1 Antibody

1. In an analysis of families with a history of colorectal cancer, we associated germline mutations in BRF1 with predisposition to colorectal cancer. Seven of the identified variants (1 detected in 2 families) affected BRF1 mRNA splicing, protein stability, or expression and/or function. PMID: 28912018
2. Site-directed mutagenesis combined with kinase assays and specific phosphosite immunodetection identified Ser-54 (S54) and Ser-334 (S334) as PKA target amino acids in vitro and in vivo. Phosphomimetic mutation of the C-terminal S334 markedly increased TIS11b half-life and, unexpectedly, enhanced TIS11b activity on mRNA decay. PMID: 27708140
3. Mutations in BRF1 cause severe short stature, remarkably delayed bone age, dysmorphic features, cerebellar hypoplasia and cognitive dysfunction inherited in an autosomal recessive pattern. PMID: 27748960
4. Brf1 expression is increased in human HCC cases, which is correlated with shorter survival times. PMID: 26701855
5. BRF1 mutations that reduce protein activity cause neurodevelopmental anomalies, suggesting that BRF1-mediated Pol III transcription is required for normal cerebellar and cognitive development. PMID: 25561519
6. hnRNP F is a co-factor in a subset of tristetraprolin/BRF1/BRF2-mediated mRNA decay. PMID: 24978456
7. these observations are in favor of a cell- and context-dependent regulation of Tis11b by hypoxia, which then contributes to modulation of angiogenesis. PMID: 21832157
8. Alcohol induces RNA polymerase III-dependent transcription through c-Jun by co-regulating TATA-binding protein (TBP) and Brf1 expression. PMID: 21106530
9. results identify a human Pol III isoform and isoform-specific functions in the regulation of cell growth and transformation PMID: 20154270
10. CK2 forms a stable complex with TFIIIB and activates RNA polymerase III transcription in human cells. PMID: 11997511
11. human small nuclear RNA gene-specific transcription factor IIIB complex de novo on and off promoter PMID: 12016223
12. BRF1 accelerated mRNA decay and antagonized the stabilizing effect of PI3-kinase, while mutation of the zinc fingers abolished both function and ARE-binding activity. This approach identifies BRF1 as an essential regulator of ARE-dependent mRNA decay. PMID: 12198173
13. Data report that protein kinase B (PKB/Akt) stabilizes ARE transcripts by phosphorylating butyrate response factor (BRF1) at serine 92. PMID: 15538381
14. These results suggest a direct role of an RNA polymerase III transcription factor in the targeting process. PMID: 16982688
15. depletion of endogenous TTP and BRF-1 proteins, or overexpression of dominant-negative mutant TTP proteins, impairs the localization of reporter AU-rich element mRNAs PMID: 17369404
16. Maf1 occupancy of Pol III genes is inversely correlated with that of the initiation factor TFIIIB (subunit Brf1) and Pol III. PMID: 17499043
17. the hypo-phosphorylated Rb appeared to be largely sequestered into a complex with Brf1, which resulted in the blockage of Rb function to repress E2F1 transactivation PMID: 17877750
18. Brf1 gene was identified in the genome-wide loss-of-function genetic screen as putative tumor suppressor located at 14q32.33. PMID: 17968325
19. MK2-mediated inhibition of BRF1 requires phosphorylation at S54, S92, and S203. PMID: 18326031
20. deregulation of Brf1 and Brf2 expression could be a key mechanism responsible for the observed deregulation of RNA pol III transcription in cancer cells PMID: 18700021

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HGNC: 11551

OMIM: 604902

KEGG: hsa:2972

STRING: 9606.ENSP00000448323

UniGene: Hs.424484