BUB1 Antibody

Code CSB-PA050227
Size US$167
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Product Details

Uniprot No. O43683
Target Names BUB1
Alternative Names Bub1 antibody; BUB1 budding uninhibited by benzimidazoles 1 homolog antibody; BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody; BUB1 mitotic checkpoint serine/threonine kinase antibody; BUB1; S. cerevisiae; homolog of antibody; BUB1_HUMAN antibody; BUB1A antibody; BUB1L antibody; Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody; Budding uninhibited by benzimidazoles 1 homolog antibody; Budding uninhibited by benzimidazoles 1; S. cerevisiae; homolog of antibody; hBUB1 antibody; Homolog of mitotic checkpoint gene BUB1 antibody; Mitotic checkpoint gene BUB1 antibody; Mitotic checkpoint serine/threonine protein kinase BUB1 antibody; Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody; Mitotic spindle checkpoint kinase antibody; Putative serine/threonine protein kinase antibody
Raised in Rabbit
Species Reactivity Human,Mouse
Immunogen Synthesized peptide derived from the Internal region of Human Bub1.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications IHC, ELISA
Recommended Dilution
Application Recommended Dilution
IHC 1:100-1:300
ELISA 1:10000
Protocols Immunohistochemistry (IHC) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Serine/threonine-protein kinase that performs 2 crucial functions during mitosis
Gene References into Functions
  1. Conserved domain 1 (CD1) in human Bub1 binds directly to Mad1 and a phosphorylation site exists in CD1 that stimulates Mad1 binding and SAC signalling. PMID: 28604727
  2. Finally, the authors show that the kinetochore localization of BUB1 and BUBR1 decreases with the age of the oocyte donors. This could contribute to oocyte aneuploidy. PMID: 28287092
  3. Small sequence differences in Bub1 and BubR1 direct Bub3 to different phosphorylated targets in the spindle assembly checkpoint signaling cascade. PMID: 28943088
  4. MiR-490-5p could regulate TGFbeta/Smad signaling pathways by inhibiting BUB1. PMID: 28810242
  5. Therefore, Mps1 promotes checkpoint activation through sequentially phosphorylating Knl1, Bub1, and Mad1. This sequential multi-target phosphorylation cascade makes the checkpoint highly responsive to Mps1 and to kinetochore-microtubule attachment. PMID: 28072388
  6. Chromosomal rearrangement resulting in BUB1 haploinsufficiency identified in a patient with polycystic liver disease. BUB1 may imply germline causes of genetic instability in PLD. PMID: 27552964
  7. Results report that BubR1 and RepoMan bind directly to PP2A-B56 using an LSPIxE short linear motif (SLiM), where phosphorylation of the Ser residue enhances binding. RepoMan and BubR1 bind B56 using both hydrophobic and electrostatic interactions. PMID: 27998540
  8. Inhibition of Bub1 kinase impaired chromosome arm resolution but exerted only minor effects on mitotic progression or spindle assembly checkpoint function. PMID: 26885717
  9. Study shows that BUB1beta and PDZ binding kinase are up-regulated in breast cancer tumors and their expression is associated with improved overall survival (OS) in basal-like tumors but worse OS in luminal and untreated patients. PMID: 26897635
  10. Bub1 may be associated with cancer stem cell potential. PMID: 26522589
  11. Both Bub1 and BubR1 bind stably to Bub3 that is required for kinetochore localization of the proteins PMID: 26260062
  12. Bub1-Plk1-mediated phosphorylation of Cdc20 constitutes an anaphase-promoting complex or cyclosome-inhibitory mechanism that is parallel, but not redundant, to mitotic checkpoint complex formation. PMID: 26912231
  13. Data show that mitotic checkpoint kinase Bub1 is an active kinase regulated by intra-molecular phosphorylation at the P+1 loop. PMID: 26658523
  14. human BUB1 does not associate stably with the MAD2 activator MAD1 (also known as MAD1L1) and, although required for accelerating the loading of MAD1 onto kinetochores, BUB1 is dispensable for the maintenance of steady-state levels of MAD1 there PMID: 26148513
  15. MAD2L1 and BUB1 may play important roles in breast cancer progression, and measuring the expression of these genes may assist the prediction of breast cancer prognosis. PMID: 26287798
  16. Data provide novel insight into the regulation and kinetochore residency of Bub1 and indicate that its localization is dynamic and tightly controlled through feedback autophosphorylation. PMID: 26399325
  17. Bub1 coordinates checkpoint signalling by distinct domains for RZZ and BubR1 recruitment and localizes antagonistic checkpoint activities. PMID: 26031201
  18. BUB1B expression was highly correlated to CDC20 and CCNB1 expression in multiple myeloma cells, leading to increased cell proliferation.Bub1 expression promotes cell proliferation and leads to poor survival in multiple myeloma patients PMID: 25698537
  19. BubR1 is a component of the Mitotic checkpoint complex and was reported to be overexpressed in Breast cancer. BubR1 overexpression was correlated with poor survival in early stage Breast cancer patients. PMID: 25731686
  20. Bub1 in complex with LANA recruits PCNA to regulate Kaposi's sarcoma-associated herpesvirus latent replication and DNA translesion synthesis. PMID: 26223641
  21. Study provides structural and functional insights into the regulation of the key mitotic kinase Bub1 and highlights a mode of substrate-specific kinase activation through intramolecular autophosphorylation of the substrate binding loop. PMID: 25308863
  22. The kinase activity of the Ser/Thr kinase BUB1 promotes TGF-beta signaling. PMID: 25564677
  23. Data show sequential multisite regulation of the microtubule-associated protein KNL1-kinase-adaptor complex BUB1/BUB3 interaction. PMID: 25661489
  24. Bub1, but not BubR1, enhances binding of Bub3 to phosphorylated kinetochores. PMID: 25611342
  25. ABBA motifs in BUBR1 and BUB1 are necessary for the spindle assembly checkpoint to work at full strength and to recruit CDC20 to kinetochore PMID: 25669885
  26. The bioinformatics analysis suggested that this variant may regulate the transcriptional ability of BUB1 PMID: 24711138
  27. BUB1 is co-expressed with AURKA and AURKB suggesting biological relationship between these spindle cell components in primary and metastatic advanced-stage ovarian serous carcinoma PMID: 24756216
  28. BUB1 and BUBR1 inhibition decreases proliferation and shows radiosensitizing effects on pediatric glioblastoma cells PMID: 23728478
  29. Bub1-mediated H2A phosphorylation penetrates kinetochores and that this histone mark contributes to a tension-sensitive Sgo1-based molecular switch for chromosome segregation. PMID: 24055156
  30. Studied the significance of budding uninhibited by benzimidazoles-1 (Bub1) and mitotic arrest deficient-2 (Mad2) expression in endometrial carcinoma. PMID: 24654460
  31. Mutations in BUB1 and BUB3 cause mosaic variegated aneuploidy and increase the risk of colorectal cancer at a young age. PMID: 23747338
  32. These results all indicated that BUB1 played important roles in the proliferation and/or progression of the breast carcinoma, and nuclear BUB1 immunohistochemical status is also considered a potent prognostic factor in human breast cancer patients. PMID: 23288590
  33. IRAK1, CHEK1 and BUB1 may play an important role in ovarian cancer. PMID: 23383610
  34. the molecular mechanism of DNA damage-induced Bub1 activation and highlight a critical role of Bub1 in DNA damage response PMID: 22071147
  35. ATM-mediated Bub1 Ser314 phosphorylation is required for Bub1 activity and is essential for the activation of the spindle checkpoint. PMID: 22099307
  36. Bub1 expression could be regulated by estradiol and paclitaxel in endometrial carcinoma Ishikawa cells. PMID: 21092550
  37. Results establish that Bub1 has oncogenic properties and suggest that Aurora B is a critical target through which overexpressed Bub1 drives aneuploidization and tumorigenesis. PMID: 21646403
  38. Findings strongly suggest that the loss of spindle assembly checkpoint proteins, such as Bub1 and Mad2, may cause spontaneous miscarriages. PMID: 20643875
  39. h-KNL1 functions to connect Bub1 and BubR1 with the hMis12, Ndc80, and Zwint-1 complexes. PMID: 21199919
  40. findings show phosphorylation of histone H3-threonine 3 mediated by Haspin cooperates with Bub1-mediated histone 2A-serine 121 phosphorylation in targeting the chromosomal passenger complex to the inner centromere in fission yeast and human cells PMID: 20929775
  41. the interaction between LANA and the kinetochore proteins CENP-F and Bub1 is important for KSHV genome tethering and its segregation to new daughter cells PMID: 20660191
  42. Sequence alterations of mitotic checkpoint genes, hBUB1 and hBUBR1, were examined, and their gene transcripts were quantified using on-line, real-time quantitative reverse transcription-PCR in surgically resected human colorectal cancers PMID: 11782350
  43. As part of a common complex in checkpoint-activated cells, Bub1 monitors different spindle events compared to BubR1 and integrates different signals into a single signal that is then relayed to the downstream cell cycle machinery. PMID: 11792804
  44. Reduced expression and aberrant transcription of the hBUB1 gene were detected in acute myeloid leukemia specimens. PMID: 11999574
  45. mutations in leukemia and lymphoma cells PMID: 12096343
  46. Results suggest that mutation of the Bub1 gene might not play a role in human stomach and oral carcinogenesis. PMID: 12375025
  47. mitotic checkpoint kinases BubR1 and BuB1, by binding to beta-adaptins, may play novel roles in the regulation of vesicular intracellular traffic PMID: 12419313
  48. The checkpoint pathway can be abrogated by mutations in BUB1 in the pancreatic cancer cell line Hs766T. PMID: 12655561
  49. No apparent selection trend was established with neoplastic progression or aneuploidy for BUB1 in Barrett esophagus. PMID: 15013707
  50. Bub1 protects centromeric cohesion through Shugoshin in mitosis PMID: 15604152
  51. Bub1 defines the persistent cohesion site along the mitotic chromosome by affecting Shugoshin localization. PMID: 15723797
  52. Bub1 is cleaved during apoptosis PMID: 15818396
  53. Data suggest that Bub1 and Aurora B function in parallel pathways that promote formation of stable bipolar kinetochore-microtubule attachments. PMID: 15933723
  54. The two spindle checkpoint arms respond to different spindle cues: whereas the Bub1 arm monitors kinetochore-microtubule attachment, the aurora B arm monitors biorientation. PMID: 16046481
  55. expression of wild-type Bub1 suppressed multinuclei formation induced by HTLV-I Tax protein both in A549 and HeLa cells. PMID: 16449967
  56. Results indicate that increased expression of the human Bub1 gene is closely linked to abnormal cell proliferation in malignant conditions. PMID: 16525682
  57. Phosphorylation of Bub1 at T609 by Cdk1 creates a docking site for the PBD of Plk1 and facilitates the kinetochore recruitment of Plk1. PMID: 16760428
  58. Bub1, an anaphase-promoting complex/ cyclosome (APC/C) inhibitor, is also an APC/C substrate. PMID: 17158872
  59. Bub1 compromise triggers p53-dependent senescence, which limits the production of aneuploid and potentially cancerous cells. PMID: 17488820
  60. Kinetochoe localization of Sgo1 depends on AurB and Bub1. PMID: 17617734
  61. BUB1 mediation of caspase-independent mitotic death determines cell fate. PMID: 17620410
  62. Bub1 is essential for postimplantation embryogenesis and proliferation of primary embryonic fibroblasts. Bub1 inactivation in adult males inhibits proliferation in seminiferous tubules, reducing sperm production and causing infertility. PMID: 17925231
  63. These observations suggest that Bub1 not only regulates the cell cycle, but also may be involved in the cytoskeletal control in interphase cells. PMID: 18036341
  64. hBUB1, hBUBR1 and hMAD2 expressions in anaplastic thyroid cancer were significantly higher than those in diffuse cancer. hBUBR1 and hMAD2 expressions in advanced DTCs were significantly higher than those in non-advanced DTCs. PMID: 18383837
  65. domain amphiphilicity is of higher importance than amino acid sequence specificity in the determination of protein adsorption and interfacial activity PMID: 18547097
  66. BUB1 and BUBR1 overexpression plays a role in cytogenetic and morphologic progression of clear cell renal cell cancer PMID: 18791270
  67. data suggest that large T antigen, via Bub1 binding, breaches genome integrity mechanisms, leading to DNA damage responses, p53 stabilization, and tetraploidy. PMID: 18922873
  68. The KEN boxes of Bub1 are specifically required for the spindle checkpoint in human cells. PMID: 18995837
  69. the spindle checkpoint kinase Bub1 contributes to the maintenance of Sgo1 steady-state protein levels in an APC/C-independent mechanism. PMID: 19015261
  70. structure provides insight into the molecular basis of Blinkin-specific recognition by BUB1 and, on a broader perspective, of the mechanism that mediates kinetochore localization of BUB1 in checkpoint-activated cells PMID: 19141287
  71. Results suggest a novel molecular mechanism leading to aneuploidy involving interference of TAp73alpha with Bub1 and Bub3 resulting in an altered mitotic checkpoint. PMID: 19182530
  72. Depletion of the outer kinetochore protein hBub1 upon activation of SAC primarily triggers early cell death mediated by p53. PMID: 19242126
  73. P53 physically interacts with hBub1 at kinetochores in response to mitotic spindle damage suggesting a direct role for hBub1 in the suppression of p53 mediated cell death. PMID: 19252416
  74. Bub1 can regulate chromosome segregation in a kinetochore-independent manner. PMID: 19487456

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Subcellular Location Nucleus, Chromosome, centromere, kinetochore
Protein Families Protein kinase superfamily, Ser/Thr protein kinase family, BUB1 subfamily
Tissue Specificity High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.
Database Links

HGNC: 1148

OMIM: 602452

KEGG: hsa:699

STRING: 9606.ENSP00000302530

UniGene: Hs.469649

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