Code | CSB-RA281837A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
CUSABIO produces the BUB1 recombinant monoclonal antibody following the four key steps: sequencing the BUB1 monoclonal antibody gene, cloning the gene into a plasmid vector, introducing the recombinant vector into a host cell line, and purifying the BUB1 recombinant monoclonal antibody from the cell culture supernatant using affinity chromatography. The BUB1 monoclonal antibody is created from the BUB1 antibody-producing hybridomas, and during its production, a synthesized peptide derived from human BUB1 is used as the immunogen. The resulting BUB1 recombinant monoclonal antibody is recommended for use in ELISA and WB applications to detect human BUB1 protein.
The BUB1 protein plays a crucial role in the spindle checkpoint pathway during cell division. It is a protein kinase that helps ensure the accurate distribution of chromosomes between daughter cells during mitosis. BUB1 acts as a sensor of improper chromosome alignment at the spindle equator and generates a signal that delays the onset of anaphase until all chromosomes are correctly attached to the mitotic spindle. BUB1 also plays a role in DNA damage response and repair, as well as in maintaining genomic stability. Mutations in the BUB1 gene have been associated with various forms of cancer and chromosomal instability disorders.
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