CCT7 Antibody

Datasheet

Code	CSB-PA859112LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: K562 whole cell lysate, 293 whole cell lysate All lanes: CCT7 antibody at 3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 60, 38, 55, 51 kDa Observed band size: 60 kDa IHC image of CSB-PA859112LA01HU diluted at 1:100 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of MCF-7 cells with CSB-PA859112LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CCT7 Polyclonal antibody

Uniprot No.

Q99832

Target Names

CCT7

Alternative Names

CCT ETA antibody; CCT-eta antibody; CCT7 antibody; CCTETA antibody; Ccth antibody; Chaperonin containing t complex polypeptide 1 eta subunit antibody; Chaperonin containing TCP1 subunit 7 (eta) antibody; Chaperonin containing TCP1 subunit 7 antibody; HIV 1 Nef interacting protein antibody; HIV-1 Nef-interacting protein antibody; Nip7 1 antibody; T complex protein 1 eta subunit antibody; T-complex protein 1 subunit eta, N-terminally processed antibody; TCP-1-eta antibody; TCP1eta antibody; TCPH_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human T-complex protein 1 subunit eta protein (240-543AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CCT7 Antibody (Product code: CSB-PA859112LA01HU) is Non-conjugated. For CCT7 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA859112LB01HU	CCT7 Antibody, HRP conjugated	ELISA
FITC	CSB-PA859112LC01HU	CCT7 Antibody, FITC conjugated
Biotin	CSB-PA859112LD01HU	CCT7 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. The TRiC complex plays a role in the folding of actin and tubulin.

Gene References into Functions

the increased expression of CCT-eta appears to be a marker for latent and active Dupuytren's contracture and to be essential for the increased contractility exhibited by these fibroblasts PMID: 23292503
Functional characterization of the CCT complex and its subunits in mouse. PMID: 11532003
A protein in mouse, that is highly similar to the one described in this record, was found to interact with a protein involved in the Nitric oxide (NO) signal transduction pathway, soluble guanylyl cyclase (sGC). PMID: 15347653

Subcellular Location

Cytoplasm.

Protein Families

TCP-1 chaperonin family

Database Links

HGNC: 1622

OMIM: 605140

KEGG: hsa:10574

STRING: 9606.ENSP00000258091

UniGene: Hs.368149

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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