CNOT3 Antibody

Datasheet

Code	CSB-PA005666LA01HU
Size	US$166
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Image	IHC image of CSB-PA005666LA01HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of Hela cells with CSB-PA005666LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CNOT3 Polyclonal antibody

Uniprot No.

O75175

Target Names

CNOT3

Alternative Names

CCR4 associated factor 3 antibody; CCR4-associated factor 3 antibody; CCR4-NOT transcription complex subunit 3 antibody; CNOT3 antibody; CNOT3_HUMAN antibody; KIAA0691 antibody; LENG2 antibody; Leukocyte receptor cluster member 2 antibody; negative regulator of transcription 3, S. cerevisiae, homolog of antibody; NOT3 (negative regulator of transcription 3, yeast) homolog antibody; NOT3 antibody; NOT3H antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human CCR4-NOT transcription complex subunit 3 protein (257-395AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CNOT3 Antibody (Product code: CSB-PA005666LA01HU) is Non-conjugated. For CNOT3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA005666LB01HU	CNOT3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA005666LC01HU	CNOT3 Antibody, FITC conjugated
Biotin	CSB-PA005666LD01HU	CNOT3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity.

Gene References into Functions

Cnot3 enhances cardiomyocyte proliferation by promoting cell cycle inhibitor mRNA degradation. PMID: 28473716
our findings implicate CNOT3 in the coordination of colonic epithelial cell self-renewal, suggesting this factor as a new biomarker for molecular and prognostic classification of early-stage colorectal cancer PMID: 27899379
A mutation in PRPF31 is hypostatic to a trait acting on CNOT3, with the RP11 phenotype only being observed when there is homozygous (recessive) inheritance of the higher expressivity CNOT3 ("symptomatic" or risk) allele. PMID: 24116917
In asymptomatic carriers CNOT3 is expressed at low levels, allowing higher amounts of wild-type PRPF31 transcripts to be produced and preventing manifestation of retinal degeneration. PMID: 23144630
CNOT3 is a tumor suppressor mutated in 7 of 89 (7.9%) adult T-cell acute lymphoblastic leukemias, and its knockdown causes tumors in a sensitized Drosophila melanogaster model. PMID: 23263491
A high density SNP genotyping approach within the 19q13 chromosome region identifies an association of a CNOT3 polymorphism with ankylosing spondylitis. PMID: 22294640
CNOT3 depletion stabilizes the MAD1 mRNA, and MAD1 knockdown attenuates the CNOT3 depletion-induced increase of the mitotic index. PMID: 22342980
TBP-interacting protein 120B binds to NOT3 PMID: 12207886

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Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, P-body.

Protein Families

CNOT2/3/5 family

Tissue Specificity

Ubiquitous. Highly expressed in brain, heart, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes.

Database Links

HGNC: 7879

OMIM: 604910

KEGG: hsa:4849

STRING: 9606.ENSP00000221232

UniGene: Hs.343571

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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