CPNE7 Antibody

Datasheet

Code	CSB-PA866203LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: U87 whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Mouse brain tissue, Rat heart tissue All lanes: CPNE7 antibody at 3.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 71, 62 kDa Observed band size: 62 kDa IHC image of CSB-PA866203LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA866203LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CPNE7 Polyclonal antibody

Uniprot No.

Q9UBL6

Target Names

CPNE7

Alternative Names

CPNE7Copine-7 antibody; Copine VII antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Copine-7 protein (254-393AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CPNE7 Antibody (Product code: CSB-PA866203LA01HU) is Non-conjugated. For CPNE7 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA866203LB01HU	CPNE7 Antibody, HRP conjugated	ELISA
FITC	CSB-PA866203LC01HU	CPNE7 Antibody, FITC conjugated
Biotin	CSB-PA866203LD01HU	CPNE7 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Calcium-dependent phospholipid-binding protein that may play a role in calcium-mediated intracellular processes.

Gene References into Functions

These results suggest Cpne7 is a diffusible signaling molecule that is secreted by preameloblasts, and regulates the differentiation of mesenchymal cells of dental or non-dental origin into odontoblasts. PMID: 25453951

Subcellular Location

Cytoplasm. Nucleus. Cell membrane.

Protein Families

Copine family

Tissue Specificity

Expressed in the brain, testis, thymus and small intestine.

Database Links

HGNC: 2320

OMIM: 605689

KEGG: hsa:27132

STRING: 9606.ENSP00000268720

UniGene: Hs.461775

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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