CPOX Antibody

Datasheet

Code	CSB-PA005910LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate All lanes: CPOX antibody at 5.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 31 kDa Observed band size: 51 kDa IHC image of CSB-PA005910LA01HU diluted at 1:300 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA005910LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) CPOX Polyclonal antibody

Uniprot No.

P36551

Target Names

CPOX

Alternative Names

Coprogen oxidase antibody; COPROPORPHYRIA antibody; Coproporphyrinogen III oxidase antibody; Coproporphyrinogen Oxidase (CPOX) antibody; Coproporphyrinogen-III oxidase antibody; Coproporphyrinogenase antibody; COX antibody; CPO antibody; Cpox antibody; CPX antibody; HEM 6 antibody; Hem-6 antibody; Hem6 antibody; HEM6_HUMAN antibody; mitochondrial antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial protein (19-149AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The CPOX Antibody (Product code: CSB-PA005910LA01HU) is Non-conjugated. For CPOX Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA005910LB01HU	CPOX Antibody, HRP conjugated	ELISA
FITC	CSB-PA005910LC01HU	CPOX Antibody, FITC conjugated
Biotin	CSB-PA005910LD01HU	CPOX Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.

Gene References into Functions

The monomer form of mutated CPOX did not show any activity and homodimeric enzymes derived from Hereditary coproporphyria (HCP) mutant showed low activity (<20% of the control). PMID: 24078084
Polymorphism of coproporphyrinogen oxidase is associated with genetic susceptibility to the adverse neurobehavioral effects of Hg exposure in children. PMID: 22765978
Deletion of the fifth exon in the CPOX gene is associated with hereditary coproporphyria. PMID: 21231929
CPOX polymorphisms are associated with biological media contamination and apoptosis disorders. PMID: 22288185
competitive action of both uroporphyrinogen decarboxylase and CPO on the same diacetate porphyrinogen substrate provides additional perspectives on the potential existence of abnormal pathways for heme biosynthesis PMID: 21277781
disease-producing mutations in the CPO gene in nine Swedish families with hereditary coproporphyria PMID: 12181641
coproporphyrinogen III oxidase sequence matches many structural features from urate oxidase PMID: 12208494
Modulation of penetrance by the wild-type allele in dominantly inherited erythrohepatic and acute hepatic porphyrias was studied using CPO. PMID: 14669009
All other type of mutations or missense mutations mapped elsewhere throughout the CPO gene, lead to coproporphyrin accumulation and subsequently typical HCP. PMID: 16159891
CPO mutations form the structural basis of hereditary coproporphyria. PMID: 16176984
The Km recognition) and Kcat values for coproporphyrinogen III and IV were determined. PMID: 16258391
His158 of human CPO may have a role in the active site, but none of the conserved histidine residues of human coproporphyrinogen oxidase is essential for catalytic activity. PMID: 17179900
The authors report the association of a novel mutation in the coproporphyrinogen oxidase gene in an Irish pedigree with the devlopment of hereditary coproporphyria PMID: 18557518
Three of the novel missense mutations and one frameshift mutation was detected in coproporphyrinogen III oxidase (CPO) gene in five Italian patients affected by Hereditary Coproporphyria (HCP). PMID: 19267996
biochemical & kinetic properties of CPOX4, the product of a polymorphism of the CPOX gene that modifies effects of mercury on neurobehavioral function; suggests CPOX4 may predispose to impaired heme biosynthesis which is limited further by Hg exposure PMID: 19339664

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Involvement in disease

Hereditary coproporphyria (HCP)

Subcellular Location

Mitochondrion intermembrane space.

Protein Families

Aerobic coproporphyrinogen-III oxidase family

Database Links

HGNC: 2321

OMIM: 121300

KEGG: hsa:1371

STRING: 9606.ENSP00000264193

UniGene: Hs.476982

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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