CSTF2 Antibody

Code CSB-PA001816
Size US$167
Image
  • Western Blot analysis of HepG2 cells using CstF-64 Polyclonal Antibody
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Product Details

Uniprot No. P33240
Target Names CSTF2
Alternative Names betaCstF 64 variant 2 antibody; CF-1 64 kDa subunit antibody; CF1 64 kDa subunit antibody; Cleavage stimulation factor 2 antibody; Cleavage stimulation factor 64 kDa subunit antibody; Cleavage stimulation factor subunit 2 antibody; Cleavage stimulation factor; 3' pre RNA; subunit 2; 64kDa antibody; CSTF 2 antibody; CstF 64 antibody; CSTF 64 kDa subunit antibody; CstF-64 antibody; CSTF2 antibody; CSTF2_HUMAN antibody; MGC188397 antibody
Raised in Rabbit
Species Reactivity Human,Mouse
Immunogen Synthesized peptide derived from the N-terminal region of Human CstF-64.
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Isotype IgG
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB, IHC, IF, ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:5000
Protocols Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity).
Gene References into Functions
  1. hnRNP H binds to two specific G-runs in exon 5a of ACHE and activates the distal alternative 3 splice site (ss) between exons 5a and 5b. Furthermore, hnRNP H competes for binding of CstF64 to the overlapping binding sites in exon 5a, and suppresses the selection of a cryptic polyadenylation site, which additionally ensures transcription of the distal 3 ss required for the generation of AChET isoform. PMID: 28180311
  2. CstF-64 is dispensable for the expression/3'-end processing of Star-PAP target mRNAs.CstF-64 and 3'-UTR cis-element determine Star-PAP specificity for target mRNA selection by excluding poly A polymerase. PMID: 26496945
  3. CstF64, an essential polyadenylation factor, is a master regulator of 3'-UTR shortening across multiple tumour types. PMID: 25409906
  4. CstF64 is central to the function of a heat-labile factor, composed of cleavage/polyadenylation specificity factor, symplekin, and cleavage stimulation factor 64 and appears to be at least partly responsible for its cell cycle regulation. PMID: 25266659
  5. CstF64 and CstF64tau modulate one another's expression and play overlapping as well as distinct roles in regulating global alternative polyadenylation profiles. PMID: 24149845
  6. CstF64 binds to thousands of dormant intronic PASs that are suppressed, at least in part, by U1 small nuclear ribonucleoproteins PMID: 23112178
  7. CSTF2 is likely to play an important role in lung carcinogenesis and be a prognostic biomarker in the clinic. PMID: 21813631
  8. nuclear accumulation of CstF-64 depends on binding to CstF-77 not symplekin; interaction between CstF-64/CstF-64Tau and CstF-77 are important for maintenance of nuclear levels of CstF complex components and intracellular localization, stability, function PMID: 21119002
  9. The Hinge domain is necessary for CstF-64 interaction with CstF-77 and consequent nuclear localization. PMID: 19887456
  10. dynamics of the CstF-64 RNA-binding domain, both free and bound to two GU-rich RNA sequences that represent polyadenylation regulatory elements, by NMR Spectroscopy PMID: 15769465
  11. The inactivity of the RSV poly(A) site was at least in part due to poor CstF binding since tethering CstF to the RSV substrate activated polyadenylation. PMID: 18272196
  12. CstF64 (cstf2) and not other CstF subunits induced by lipopolysaccharide (LPS) in murine macrophages and changes polyA site use PMID: 16207706
  13. Describes the induction of CSTF2 in the growth phase of cells. PMID: 9736695

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Subcellular Location Nucleus
Database Links

HGNC: 2484

OMIM: 300907

KEGG: hsa:1478

STRING: 9606.ENSP00000362063

UniGene: Hs.132370

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