DACT1 Antibody

Datasheet

Code	CSB-PA873691LA01HU
Size	US$166
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Image	IHC image of CSB-PA873691LA01HU diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA873691LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DACT1 Polyclonal antibody

Uniprot No.

Q9NYF0

Target Names

DACT1

Alternative Names

Dact antibody; dact1 antibody; DACT1_HUMAN antibody; Dapper antagonist of beta catenin homolog 1 antibody; Dapper antagonist of catenin 1 antibody; Dapper antibody; Dapper homolog 1 antibody; DAPPER1 antibody; Dpr antibody; Dpr1 antibody; Frd antibody; Frodo antibody; hDPR1 antibody; Hepatocellular carcinoma novel gene 3 protein antibody; Heptacellular carcinoma novel gene 3 antibody; HNG3 antibody; MTNG3 antibody; Thyex3 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Dapper homolog 1 protein (410-525AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DACT1 Antibody (Product code: CSB-PA873691LA01HU) is Non-conjugated. For DACT1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA873691LB01HU	DACT1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA873691LC01HU	DACT1 Antibody, FITC conjugated
Biotin	CSB-PA873691LD01HU	DACT1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Involved in regulation of intracellular signaling pathways during development. Specifically thought to play a role in canonical and/or non-canonical Wnt signaling pathways through interaction with DSH (Dishevelled) family proteins. The activation/inhibition of Wnt signaling may depend on the phosphorylation status. Proposed to regulate the degradation of CTNNB1/beta-catenin, thereby modulating the transcriptional activation of target genes of the Wnt signaling pathway. Its function in stabilizing CTNNB1 may involve inhibition of GSK3B activity. Promotes the membrane localization of CTNNB1. The cytoplasmic form can induce DVL2 degradation via a lysosome-dependent mechanism; the function is inhibited by PKA-induced binding to 14-3-3 proteins, such as YWHAB. Seems to be involved in morphogenesis at the primitive streak by regulating VANGL2 and DVL2; the function seems to be independent of canonical Wnt signaling and rather involves the non-canonical Wnt/planar cell polarity (PCP) pathway. The nuclear form may prevent the formation of LEF1:CTNNB1 complex and recruit HDAC1 to LEF1 at target gene promoters to repress transcription thus antagonizing Wnt signaling. May be involved in positive regulation of fat cell differentiation. During neuronal differentiation may be involved in excitatory synapse organization, and dendrite formation and establishment of spines.

Gene References into Functions

DACT1alpha plays a pivotal role as a potential tumor suppressor in migration and invasion of gastric cancer. DACT1alpha methylation may serve as a biomarker for the prognosis of gastric cancer. PMID: 27833078
Findings suggest that the DACT1 c.1256G>A nonsense variant is causative of a specific genetic syndrome with features overlapping Townes-Brocks syndrome. PMID: 28054444
Dapper1 attenuates hepatic gluconeogenesis and lipogenesis in Ttype 2 diabetes. PMID: 28237722
An inhibitory role for DACT1 in leukemogenesis. PMID: 29037126
Dact1 is up-regulated by TGF-beta1, inducing apoptosis in mesangial cells. PMID: 27714812
The simultaneous methylation of DACT1 and DACT2 may play important roles in progression of ESCC and may serve as prognostic methylation biomarkers for ESCC patients. PMID: 28077137
Dact1 has a critical role in the ability support keratinocyte proliferation, by attenuating Wnt/beta catenin signaling. PMID: 26099026
our results suggested that DACT1 was upregulated during human placenta development. PMID: 25424899
There was no statistical difference between groups concerning DACT1 and DACT2 either in promoter hypermethylation or transcript levels. Age was associated with DACT2 promoter hypermethylation, especially over 56 years old. PMID: 25524937
Dpr1 promotes the ubiquitination of Dvl2 by pVHL and mediates the protein aggregate-elicited autophagy initiation PMID: 25825496
Overexpression of Dapper-1 allows the translocation of MIZ-1 from the nucleus to the cytoplasm. PMID: 25558878
Dpr1 directly interacts with Beclin1 and Atg14L and enhances the Beclin1-Vps34 interaction and Vps34 activity. PMID: 24980960
Data indicate that Sestd1 cooperates with Dact1 in Vangl2 regulation and in the planar cell polarity (PCP) pathway during mammalian embryonic development. PMID: 23696638
five missense heterozygote mutations of the DACT1 gene are specifically identified in 167 stillborn or miscarried Han Chinese fetuses with neural tube defects. PMID: 22610794
These findings provided insight into the role of DACT1 as a novel functional tumor suppressor in gastric cancer through inhibiting NF-kappaB signaling pathway. PMID: 23073659
DACT1 stabilizes beta-catenin via DACT1-induced effects on GSK-3beta and directly interacts with beta-catenin proteins. PMID: 22470507
As(2)O(3) induces demethylation of hdpr1 gene from abnormal hypermethylation status and activates its reexpression, thus suppressing the proliferation of Jurkat cells. PMID: 21176356
Cytoplasmic HDPR1 protein expression was associated with tumor malignant progression via beta-catenin accumulation. PMID: 21525190
14-3-3beta interacts with human Dapper1, attenuating the ability of hDpr1 to promote Dishevelled (Dvl) degradation, thus enhancing Wnt signaling PMID: 21262972
Knockdown of HDPR1 gene enhanced the invasive ability of lung cancer cells, which was dependent on p120ctn and independent of beta-catenin PMID: 20232357
Downregulation of HDPR1 is common in hepatic cellular carcinomas, frequently involves hypermethylation of the promoter region. PMID: 15580286
DACT1 antagonizes Wnt signaling by promoting DVL2 degradation. PMID: 16446366
Dact1 regulates adipogenesis through coordinated effects on gene expression that selectively alter intracellular and paracrine/autocrine components of the Wnt/beta-catenin signaling pathway. PMID: 19073771

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Involvement in disease

Neural tube defects (NTD); Townes-Brocks syndrome 2 (TBS2)

Subcellular Location

Cytoplasm. Nucleus. Cell junction, synapse. Note=Shuttles between the nucleus and the cytoplasm. Seems to be nuclear in the absence of Wnt signaling and to translocate to the cytoplasm in its presence.

Protein Families

Dapper family

Database Links

HGNC: 17748

OMIM: 182940

KEGG: hsa:51339

STRING: 9606.ENSP00000337439

UniGene: Hs.48950

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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