DCLRE1B Antibody

Datasheet
Code CSB-PA880993LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q9H816
Image
  • Immunofluorescence staining of MCF-7 cells with CSB-PA880993LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human 5' exonuclease Apollo protein (345-493AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IF; Recommended dilution: IF:1:50-1:200
Relevance 5'-3' exonuclease that plays a central role in telomere maintenance and protection during S-phase. Participates in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair, thereby ensuring that telomeres do not fuse. Plays a key role in telomeric loop (T loop) formation by being recruited by TERF2 at the leading end telomeres and by processing leading-end telomeres immediately after their replication via its exonuclease activity: generates 3' single-stranded overhang at the leading end telomeres avoiding blunt leading-end telomeres that are vulnerable to end-joining reactions and expose the telomere end in a manner that activates the DNA repair pathways. Together with TERF2, required to protect telomeres from replicative damage during replication by controlling the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Also involved in response to DNA damage: plays a role in response to DNA interstrand cross-links (ICLs) by facilitating double-strand break formation. In case of spindle stress, involved in prophase checkpoint.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias 5' exonuclease Apollo (EC 3.1.-.-) (DNA cross-link repair 1B protein) (SNM1 homolog B) (SNMIB) (hSNM1B), DCLRE1B, SNM1B
Immunogen Species Human
Research Area Others
Target Names DCLRE1B
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function 5'-3' exonuclease that plays a central role in telomere maintenance and protection during S-phase. Participates in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair, thereby ensuring that telomeres do not fuse. Plays a key role in telomeric loop (T loop) formation by being recruited by TERF2 at the leading end telomeres and by processing leading-end telomeres immediately after their replication via its exonuclease activity
Involvement in disease Hoyeraal-Hreidarsson syndrome (HHS)
Subcellular Location Chromosome, telomere, Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Protein Families DNA repair metallo-beta-lactamase (DRMBL) family
Database Links

HGNC: 17641

OMIM: 305000

KEGG: hsa:64858

STRING: 9606.ENSP00000358576

UniGene: Hs.591412

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