DNPEP Antibody

Datasheet

Code	CSB-PA007087LA01HU
Size	US$166
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Image	IHC image of CSB-PA007087LA01HU diluted at 1:600 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of MCF-7 cells with CSB-PA007087LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DNPEP Polyclonal antibody

Uniprot No.

Q9ULA0

Target Names

DNPEP

Alternative Names

Aspartyl aminopeptidase antibody; ASPEP antibody; DAP antibody; DNPEP antibody; DNPEP_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Aspartyl aminopeptidase protein (279-386AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DNPEP Antibody (Product code: CSB-PA007087LA01HU) is Non-conjugated. For DNPEP Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA007087LB01HU	DNPEP Antibody, HRP conjugated	ELISA
FITC	CSB-PA007087LC01HU	DNPEP Antibody, FITC conjugated
Biotin	CSB-PA007087LD01HU	DNPEP Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Aminopeptidase with specificity towards an acidic amino acid at the N-terminus. Likely to play an important role in intracellular protein and peptide metabolism.

Gene References into Functions

aspartyl aminopeptidase was upregulated in colorectal cancer tissues, and greater activity correlated significantly with the absence of lymph node metastases and with better overall survival PMID: 23948443
The crystal structure of human DNPEP complexed with zinc and a substrate analogue aspartate-beta-hydroxamate reveals a dodecameric machinery built by domain-swapped dimers. PMID: 22720794
Identification of histidine residues important in the catalysis and structure of aspartyl aminopeptidase PMID: 12413488

Subcellular Location

Cytoplasm.

Protein Families

Peptidase M18 family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 2981

OMIM: 611367

KEGG: hsa:23549

STRING: 9606.ENSP00000273075

UniGene: Hs.258551

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Service

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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