EIF3G Antibody

Datasheet

Citations (1)

Code	CSB-PA03099A0Rb
Size	US$166
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Image	IHC image of CSB-PA03099A0Rb diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA03099A0Rb diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA03099A0Rb at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
Citations
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EIF3G Polyclonal antibody

Uniprot No.

O75821

Target Names

EIF3G

Alternative Names

44kDa antibody; Anti EIF3S4 antibody; eIF 3 delta antibody; eIF 3 RNA binding subunit antibody; eIF-3 RNA-binding subunit antibody; eIF-3-delta antibody; eIF3 delta antibody; eIF3 p42 antibody; eIF3 p44 antibody; eIF3g antibody; EIF3G_HUMAN antibody; EIF3S4 antibody; EIF3S4 antibody antibody; EIF3S4; formerly antibody; Eukaryotic translation initiation factor 3 antibody; Eukaryotic translation initiation factor 3 RNA binding subunit antibody; Eukaryotic translation initiation factor 3 RNA-binding subunit antibody; Eukaryotic translation initiation factor 3 subunit 4 antibody; Eukaryotic translation initiation factor 3 subunit 4 delta 44 kD antibody; Eukaryotic translation initiation factor 3 subunit G antibody; Eukaryotic translation initiation factor 3 subunit p42 antibody; Eukaryotic translation initiation factor 3; subunit 4; formerly antibody; Subunit 4 (delta 44kD) antibody; Subunit 4 delta antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Eukaryotic translation initiation factor 3 subunit G protein (2-320AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The EIF3G Antibody (Product code: CSB-PA03099A0Rb) is Non-conjugated. For EIF3G Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA03099B0Rb	EIF3G Antibody, HRP conjugated	ELISA
FITC	CSB-PA03099C0Rb	EIF3G Antibody, FITC conjugated
Biotin	CSB-PA03099D0Rb	EIF3G Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection Garcia-Moreno M, et al,Molecular Cell,2019

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Target Background

Function

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit can bind 18S rRNA.; (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2.

Gene References into Functions

The disease-associated allele increases EIF3G mRNA expression. EIF3G is located in the narcolepsy risk locus and EIF3G expression correlates with PPAN and P2RY11 expression. PMID: 25669430
Important roles for eIF3g in the translation initiation machinery and in DNA degradation during apoptosis. PMID: 24080033
down-regulation of eIF3g inhibits the efficiency of nonsense-mediated mRNA decay, which is tightly coupled to CT but not to ET PMID: 22493286
Furthermore, we found that overexpression of DISC1 in SH-SY5Y cells induces the assembly of eIF3- and TIA-1-positive stress granules (SGs), discrete cytoplasmic granules formed in response to environmental stresses. PMID: 16243297
AIF overexpression specifically resulted in the activation of caspase-7, thereby amplifying the inhibition of protein PMID: 17094969

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Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Colocalizes with AIFM1 in the nucleus and perinuclear region.

Protein Families

EIF-3 subunit G family

Database Links

HGNC: 3274

OMIM: 603913

KEGG: hsa:8666

STRING: 9606.ENSP00000253108

UniGene: Hs.529059

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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