FBXO25 Antibody

Code CSB-PA848401LA01HU
Size US$166
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  • Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA848401LA01HU at dilution of 1:100
  • Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA848401LA01HU at dilution of 1:100
  • Immunofluorescent analysis of Hela cells using CSB-PA848401LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
  • Immunofluorescent analysis of HepG2 cells using CSB-PA848401LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) FBXO25 Polyclonal antibody
Uniprot No.
Target Names
FBXO25
Alternative Names
F box only protein 25 antibody; F box protein 25 antibody; F box protein Fbx25 antibody; F-box only protein 25 antibody; FBX25 antibody; FBX25_HUMAN antibody; FBXO25 antibody; MGC20256 antibody; MGC51975 antibody; OTTHUMP00000115399 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human F-box only protein 25 protein (1-291AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The FBXO25 Antibody (Product code: CSB-PA848401LA01HU) is Non-conjugated. For FBXO25 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA848401LB01HU FBXO25 Antibody, HRP conjugated ELISA
FITC CSB-PA848401LC01HU FBXO25 Antibody, FITC conjugated
Biotin CSB-PA848401LD01HU FBXO25 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT).
Gene References into Functions
  1. Taken together we show that FBXO25 functions as a negative regulator of MAPK signaling though the reduction of ERK1/2 activation. PMID: 28389297
  2. High FBXO25 expression is associated with non-small-cell lung cancer. PMID: 27596142
  3. FBXO25 functions as a haploinsufficient tumor suppressor in mantle cell lymphoma. PMID: 25419709
  4. FBXO25 mediates ELK-1 degradation through the ubiquitin proteasome system and thereby plays a role in regulating the activation of ELK-1 pathway in response to mitogens PMID: 23940030
  5. Altogether, Fbxo25 acts as an ubiquitin E3 ligase to target cardiac transcription factors including Nkx2-5, Isl1, and Hand1, indicating that cardiac protein homeostasis through Fbxo25 has a pivotal impact on cardiac development. PMID: 21596019
  6. Beta-actin, physically interacts through its N-terminus with FBXO25 and is enriched in the FBXO25 nuclear compartments. PMID: 20473970
  7. investigated hFBX25's genomic organization and established hFBX25 as an FBP by verifying its interaction with Skp1 and Cul1 PMID: 16278047
  8. The cellular distribution of FBXO25 and its colocalization with some nuclear proteins is investigated by using immunochemical and biochemical approaches. PMID: 18287534

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Involvement in disease
A chromosomal aberration involving FBXO25 is a cause of X-linked mental retardation (XLMR). Translocation t(X;8)(p11.22;p23.3) with SHROOM4.
Subcellular Location
Nucleus. Note=In the nucleus, associates with a subnuclear dot-like structure. Colocalized with SKP1.
Tissue Specificity
Expressed in all brain tissue observed.
Database Links

HGNC: 13596

OMIM: 609098

KEGG: hsa:26260

STRING: 9606.ENSP00000276326

UniGene: Hs.438454

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