FREM2 Antibody

Datasheet

Code	CSB-PA722558LA01HU
Size	US$166
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Image	IHC image of CSB-PA722558LA01HU diluted at 1:400 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of MCF-7 cells with CSB-PA722558LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FREM2 Polyclonal antibody

Uniprot No.

Q5SZK8

Target Names

FREM2

Alternative Names

DKFZp781I048 antibody; ECM3 homolog antibody; FRAS1-related extracellular matrix protein 2 antibody; FREM 2 antibody; Frem2 antibody; FREM2_HUMAN antibody; KIAA1074 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human FRAS1-related extracellular matrix protein 2 protein (1986-2127AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The FREM2 Antibody (Product code: CSB-PA722558LA01HU) is Non-conjugated. For FREM2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA722558LB01HU	FREM2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA722558LC01HU	FREM2 Antibody, FITC conjugated
Biotin	CSB-PA722558LD01HU	FREM2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Extracellular matrix protein required for maintenance of the integrity of the skin epithelium and for maintenance of renal epithelia. Required for epidermal adhesion. Involved in the development of eyelids and the anterior segment of the eyeballs.

Gene References into Functions

FREM2 is thus proposed as a novel GB biomarker and a putative biomarker of glioblastoma stem cells. Both FREM2 and SPRY1 are expressed on the surface of the GB cells, while SPRY1 alone was found overexpressed in the cytosol of non-malignant astrocytes. PMID: 29734672
In 15 of 590 families, we identified recessive mutations in the genes FRAS1, FREM2, GRIP1, FREM1, ITGA8, and GREM1, all of which function in the interaction of the ureteric bud and the metanephric mesenchyme. PMID: 24700879
Amplification of the FREM2 gene is associated with mesenchymal differentiation in gliosarcoma. PMID: 22538188
Heterozygous missense mutations in FREM2 cause non-syndromic congenital abnormalities of the kidney and urinary tract in humans. PMID: 21900877
report on 2 fetuses affected by Fraser syndrome (FS); a homozygous IVS14 + 1G -- A mutation in FREM2 was indentified; present report provides additional evidence that FS may be caused by complete or near-complete lack or loss of function of FREM2 protein PMID: 18203166
1 new mutation in FREM2 was identified in families with Fraser syndrome. PMID: 18671281

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Involvement in disease

Fraser syndrome 2 (FRASRS2)

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

FRAS1 family

Database Links

HGNC: 25396

OMIM: 608945

KEGG: hsa:341640

STRING: 9606.ENSP00000280481

UniGene: Hs.253994

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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