HSD3B7 Antibody

Datasheet

Code	CSB-PA861999LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse liver tissue, Mouse kidney tissue All lanes: HSD3B7 antibody at 3.5μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 22 kDa Observed band size: 42 kDa Immunofluorescent analysis of HepG2 cells using CSB-PA861999LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) HSD3B7 Polyclonal antibody

Uniprot No.

Q9H2F3

Target Names

HSD3B7

Alternative Names

HSD3B7; 3 beta-hydroxysteroid dehydrogenase type 7; 3 beta-hydroxysteroid dehydrogenase type VII; 3-beta-HSD VII; 3-beta-hydroxy-Delta(5-C27 steroid oxidoreductase; C(27 3-beta-HSD; Cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human 3 beta-hydroxysteroid dehydrogenase type 7 protein (131-197AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The HSD3B7 Antibody (Product code: CSB-PA861999LA01HU) is Non-conjugated. For HSD3B7 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA861999LB01HU	HSD3B7 Antibody, HRP conjugated	ELISA
FITC	CSB-PA861999LC01HU	HSD3B7 Antibody, FITC conjugated
Biotin	CSB-PA861999LD01HU	HSD3B7 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids. HSD VII is active against four 7-alpha-hydroxylated sterols. Does not metabolize several different C(19/21) steroids as substrates. Involved in bile acid synthesis. Plays a key role in cell positioning and movement in lymphoid tissues by mediating degradation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC): 7-alpha,25-OHC acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells.

Gene References into Functions

Novel Mutations in the 3beta-hydroxy-5-C27-steroid Dehydrogenase Gene (HSD3B7) in a Patient with Neonatal Cholestasis. PMID: 26712441
Expression of steroid sulfated transporters and 3beta-HSD activity in endometrium of polycystic ovary syndrome PMID: 26450365
Homozygosity mapping identifies a bile acid biosynthetic defect (3beta-HSD deficiency due to a frameshift mutation in HSD3B7) in an adult with cirrhosis of unknown etiology. PMID: 22095780
Mutations in the HSD3B7 gene account for autosomal recessive neonatal cholestasis caused by 3[beta]-hydroxy-[DELTA]5-C27-steroid dehydrogenase/isomerase deficiency. PMID: 20531254

Involvement in disease

Congenital bile acid synthesis defect 1 (CBAS1)

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

3-beta-HSD family

Database Links

HGNC: 18324

OMIM: 607764

KEGG: hsa:80270

STRING: 9606.ENSP00000297679

UniGene: Hs.460618

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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